CHAC1 Antibody - #DF9353

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产品: CHAC1 抗体
货号: DF9353
描述: Rabbit polyclonal antibody to CHAC1
应用: WB IHC IF/ICC
反应: Human
预测: Pig, Zebrafish, Bovine, Horse, Sheep, Rabbit, Dog, Chicken, Xenopus
分子量: 29kDa; 24kD(Calculated).
蛋白号: Q9BUX1
RRID: AB_2842549

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   规格 价格 库存
 50ul RMB¥ 1250 现货
 100ul RMB¥ 2300 现货
 200ul RMB¥ 3000 现货

货期: 当天发货

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MS Report
HPLC Report
MSDS
说明书
COA
实验方案
WB Handbook
IHC Protocol
IF/ICC Protocol

产品描述

来源:
Rabbit
应用:
IHC 1:50-1:200, WB 1:1000-3000, IF/ICC 1:100-1:500
*The optimal dilutions should be determined by the end user.
*Tips:

WB: 适用于变性蛋白样本的免疫印迹检测. IHC: 适用于组织样本的石蜡(IHC-p)或冰冻(IHC-f)切片样本的免疫组化/荧光检测. IF/ICC: 适用于细胞样本的荧光检测. ELISA(peptide): 适用于抗原肽的ELISA检测.

反应:
Human
预测:
Pig(100%), Zebrafish(80%), Bovine(100%), Horse(100%), Sheep(100%), Rabbit(100%), Dog(100%), Chicken(83%), Xenopus(83%)
克隆:
Polyclonal
特异性:
CHAC1 Antibody detects endogenous levels of total CHAC1.
RRID:
AB_2842549
引用格式: Affinity Biosciences Cat# DF9353, RRID:AB_2842549.
偶联:
Unconjugated.
纯化:
The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).
保存:
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
别名:

展开/折叠

Blocks Notch protein; Botch; Cation transport regulator like protein 1; Cation transport regulator-like protein 1; ChaC; ChaC cation transport regulator homolog 1; ChaC cation transport regulator like 1; chac1; CHAC1_HUMAN; Gamma GCT acting on glutathione homolog 1; Gamma-GCG 1; Glutathione-specific gamma-glutamylcyclotransferase 1;

抗原和靶标

免疫原:
Uniprot:

Q9BUX1(CHAC1_HUMAN) >>Visit HPA database.

基因/基因ID:
CHAC1(79094)
序列:
MKQESAAPNTPPTSQSPTPSAQFPRNDGDPQALWIFGYGSLVWRPDFAYSDSRVGFVRGYSRRFWQGDTFHRGSDKMPGRVVTLLEDHEGCTWGVAYQVQGEQVSKALKYLNVREAVLGGYDTKEVTFYPQDAPDQPLKALAYVATPQNPGYLGPAPEEAIATQILACRGFSGHNLEYLLRLADFMQLCGPQAQDEHLAAIVDAVGTMLPCFCPTEQALALV

种属预测

种属预测:

score>80的预测可信度较高，可尝试用于WB检测。*预测模型主要基于免疫原序列比对，结果仅作参考，不作为质保凭据。

Species
Results
Score
Pig
100
Horse
100
Bovine
100
Sheep
100
Dog
100
Rabbit
100
Xenopus
83
Chicken
83
Zebrafish
80
Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

翻译修饰 - Q9BUX1 作为底物

Site PTM Type Enzyme
S61 Phosphorylation
T92 Phosphorylation
K109 Sumoylation
K109 Ubiquitination
K124 Ubiquitination
S172 Phosphorylation

研究背景

功能:

Catalyzes the cleavage of glutathione into 5-oxo-L-proline and a Cys-Gly dipeptide. Acts specifically on glutathione, but not on other gamma-glutamyl peptides. Glutathione depletion is an important factor for apoptosis initiation and execution. Acts as a pro-apoptotic component of the unfolded protein response pathway by mediating the pro-apoptotic effects of the ATF4-ATF3-DDIT3/CHOP cascade. Negative regulator of Notch signaling pathway involved in embryonic neurogenesis: acts by inhibiting Notch cleavage by furin, maintaining Notch in an immature inactive form, thereby promoting neurogenesis in embryos.

细胞定位:

Cytoplasm>Cytosol. Golgi apparatus>trans-Golgi network.

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionSubcellular location
亚基结构:

Interacts with NOTCH1 (via extracellular region).

蛋白家族:

Belongs to the gamma-glutamylcyclotransferase family. ChaC subfamily.

文献引用

1). Identification of Ferroptotic Genes in Spinal Cord Injury at Different Time Points: Bioinformatics and Experimental Validation. Molecular Neurobiology, 2022 (PubMed: 35796899) [IF=5.1]
2). CPEB1 enhances erastin‐induced ferroptosis in gastric cancer cells by suppressing twist1 expression. IUBMB LIFE, 2021 (PubMed: 34184391) [IF=4.6]

Application: WB    Species: Mice    Sample: GC cells

FIGURE 4 CPEB1 suppressed twist1 expression in GC cells. (a-f) After CPEB1-silenced AGS cells and CPEB1-overexpressed HGC-27 cells were treated with erastin (10 μg/ml) for 24 h, twist1, ATF4, and CHAC1 protein expression levels were measured by western blot. N = 3. Data are shown as means ± SD. **p < 0.01, and ***p < 0.001. ATF4, activating transcription factor 4; CPEB1, cytoplasmic polyadenylation element binding protein 1; CHAC1, ChaC glutathione-specific γ glutamylcyclotransferase 1; GC, gastric cancer

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