产品描述
*The optimal dilutions should be determined by the end user.
*Tips:
WB: 适用于变性蛋白样本的免疫印迹检测. IHC: 适用于组织样本的石蜡(IHC-p)或冰冻(IHC-f)切片样本的免疫组化/荧光检测. IF/ICC: 适用于细胞样本的荧光检测. ELISA(peptide): 适用于抗原肽的ELISA检测.
引用格式: Affinity Biosciences Cat# DF9369, RRID:AB_2842565.
展开/折叠
CERU_HUMAN; Ceruloplasmin; CP 2; CP; CP2; Ferroxidase;
抗原和靶标
- P00450 CERU_HUMAN:
- Protein BLAST With
- NCBI/
- ExPASy/
- Uniprot
MKILILGIFLFLCSTPAWAKEKHYYIGIIETTWDYASDHGEKKLISVDTEHSNIYLQNGPDRIGRLYKKALYLQYTDETFRTTIEKPVWLGFLGPIIKAETGDKVYVHLKNLASRPYTFHSHGITYYKEHEGAIYPDNTTDFQRADDKVYPGEQYTYMLLATEEQSPGEGDGNCVTRIYHSHIDAPKDIASGLIGPLIICKKDSLDKEKEKHIDREFVVMFSVVDENFSWYLEDNIKTYCSEPEKVDKDNEDFQESNRMYSVNGYTFGSLPGLSMCAEDRVKWYLFGMGNEVDVHAAFFHGQALTNKNYRIDTINLFPATLFDAYMVAQNPGEWMLSCQNLNHLKAGLQAFFQVQECNKSSSKDNIRGKHVRHYYIAAEEIIWNYAPSGIDIFTKENLTAPGSDSAVFFEQGTTRIGGSYKKLVYREYTDASFTNRKERGPEEEHLGILGPVIWAEVGDTIRVTFHNKGAYPLSIEPIGVRFNKNNEGTYYSPNYNPQSRSVPPSASHVAPTETFTYEWTVPKEVGPTNADPVCLAKMYYSAVDPTKDIFTGLIGPMKICKKGSLHANGRQKDVDKEFYLFPTVFDENESLLLEDNIRMFTTAPDQVDKEDEDFQESNKMHSMNGFMYGNQPGLTMCKGDSVVWYLFSAGNEADVHGIYFSGNTYLWRGERRDTANLFPQTSLTLHMWPDTEGTFNVECLTTDHYTGGMKQKYTVNQCRRQSEDSTFYLGERTYYIAAVEVEWDYSPQREWEKELHHLQEQNVSNAFLDKGEFYIGSKYKKVVYRQYTDSTFRVPVERKAEEEHLGILGPQLHADVGDKVKIIFKNMATRPYSIHAHGVQTESSTVTPTLPGETLTYVWKIPERSGAGTEDSACIPWAYYSTVDQVKDLYSGLIGPLIVCRRPYLKVFNPRRKLEFALLFLVFDENESWYLDDNIKTYSDHPEKVNKDDEEFIESNKMHAINGRMFGNLQGLTMHVGDEVNWYLMGMGNEIDLHTVHFHGHSFQYKHRGVYSSDVFDIFPGTYQTLEMFPRTPGIWLLHCHVTDHIHAGMETTYTVLQNEDTKSG
种属预测
score>80的预测可信度较高，可尝试用于WB检测。*预测模型主要基于免疫原序列比对，结果仅作参考，不作为质保凭据。
High(score>80) Medium(80>score>50) Low(score<50) No confidence
翻译修饰 - P00450 作为底物
| Site | PTM Type | Enzyme | Source |
|---|---|---|---|
| Y72 | Phosphorylation | Uniprot | |
| N138 | N-Glycosylation | Uniprot | |
| S204 | Phosphorylation | Uniprot | |
| K209 | Ubiquitination | Uniprot | |
| N358 | N-Glycosylation | Uniprot | |
| N397 | N-Glycosylation | Uniprot | |
| Y428 | Phosphorylation | Uniprot | |
| T429 | Phosphorylation | Uniprot | |
| S432 | Phosphorylation | Uniprot | |
| T434 | Phosphorylation | Uniprot | |
| S492 | O-Glycosylation | Uniprot | |
| S492 | Phosphorylation | Uniprot | |
| S499 | Phosphorylation | Uniprot | |
| Y539 | Phosphorylation | Uniprot | |
| N588 | N-Glycosylation | Uniprot | |
| S722 | Phosphorylation | Uniprot | |
| S725 | Phosphorylation | Uniprot | |
| N762 | N-Glycosylation | Uniprot | |
| S764 | Phosphorylation | Uniprot | |
| Y784 | Phosphorylation | Uniprot | |
| Y787 | Phosphorylation | Uniprot | |
| K906 | Ubiquitination | Uniprot | |
| N926 | N-Glycosylation | Uniprot |
研究背景
Ceruloplasmin is a blue, copper-binding (6-7 atoms per molecule) glycoprotein. It has ferroxidase activity oxidizing Fe(2+) to Fe(3+) without releasing radical oxygen species. It is involved in iron transport across the cell membrane. Provides Cu(2+) ions for the ascorbate-mediated deaminase degradation of the heparan sulfate chains of GPC1. May also play a role in fetal lung development or pulmonary antioxidant defense (By similarity).
Secreted.
Note: Colocalizes with GCP1 in secretory intracellular compartments.
Expressed by the liver and secreted in plasma.
Belongs to the multicopper oxidase family.
研究领域
· Cellular Processes > Cell growth and death > Ferroptosis. (View pathway)
· Metabolism > Metabolism of cofactors and vitamins > Porphyrin and chlorophyll metabolism.
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