PDE3B Antibody - #DF9372

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产品: PDE3B 抗体
货号: DF9372
描述: Rabbit polyclonal antibody to PDE3B
应用: WB IHC IF/ICC
反应: Human
预测: Pig, Bovine, Horse, Sheep, Rabbit, Dog, Xenopus
分子量: 124kDa; 124kD(Calculated).
蛋白号: Q13370
RRID: AB_2842568

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   规格 价格 库存
 100ul RMB¥ 2300 现货
 200ul RMB¥ 3000 现货

货期: 当天发货

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MSDS
说明书
COA
实验方案
WB Handbook
IHC Protocol
IF/ICC Protocol

产品描述

来源:
Rabbit
应用:
IF/ICC 1:100-1:500, IHC 1:50-1:200, WB 1:1000-3000
*The optimal dilutions should be determined by the end user.
*Tips:

WB: 适用于变性蛋白样本的免疫印迹检测. IHC: 适用于组织样本的石蜡(IHC-p)或冰冻(IHC-f)切片样本的免疫组化/荧光检测. IF/ICC: 适用于细胞样本的荧光检测. ELISA(peptide): 适用于抗原肽的ELISA检测.

反应:
Human
预测:
Pig(88%), Bovine(%), Horse(%), Sheep(%), Rabbit(%), Dog(%), Xenopus(%)
克隆:
Polyclonal
特异性:
PDE3B Antibody detects endogenous levels of total PDE3B.
RRID:
AB_2842568
引用格式: Affinity Biosciences Cat# DF9372, RRID:AB_2842568.
偶联:
Unconjugated. 130
纯化:
The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).
保存:
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
别名:

展开/折叠

5''-cyclic phosphodiesterase B; Adipocyte cAMP phosphodiesterase cGMP inhibited; AI847709; cAMP Phosphodiesterase, adipocyte, cGMP-inhibited; CGI-PDE B; CGIP1; CGIPDE B; CGIPDE1; cGMP inhibited 3 5 cyclic phosphodiesterase B; CGMP inhibited cAMP phophodiesterase; cGMP-inhibited 3''; Cyclic GMP inhibited phosphodiesterase B; Cyclic GMP-inhibited phosphodiesterase B; Cyclic nucleotide phosphodiesterase; HcGIP1; HcGIP1, rat, homolog of; Pde3b; PDE3B_HUMAN; Phosphodiesterase 3B; Phosphodiesterase 3B cGMP inhibited;

抗原和靶标

免疫原:
Uniprot:

Q13370(PDE3B_HUMAN) >>Visit HPA database.

基因/基因ID:
PDE3B(5140)
表达:
Q13370 PDE3B_HUMAN:

Abundant in adipose tissues.

序列:
MRRDERDAKAMRSLQPPDGAGSPPESLRNGYVKSCVSPLRQDPPRGFFFHLCRFCNVELRPPPASPQQPRRCSPFCRARLSLGALAAFVLALLLGAEPESWAAGAAWLRTLLSVCSHSLSPLFSIACAFFFLTCFLTRTKRGPGPGRSCGSWWLLALPACCYLGDFLVWQWWSWPWGDGDAGSAAPHTPPEAAAGRLLLVLSCVGLLLTLAHPLRLRHCVLVLLLASFVWWVSFTSLGSLPSALRPLLSGLVGGAGCLLALGLDHFFQIREAPLHPRLSSAAEEKVPVIRPRRRSSCVSLGETAASYYGSCKIFRRPSLPCISREQMILWDWDLKQWYKPHYQNSGGGNGVDLSVLNEARNMVSDLLTDPSLPPQVISSLRSISSLMGAFSGSCRPKINPLTPFPGFYPCSEIEDPAEKGDRKLNKGLNRNSLPTPQLRRSSGTSGLLPVEQSSRWDRNNGKRPHQEFGISSQGCYLNGPFNSNLLTIPKQRSSSVSLTHHVGLRRAGVLSSLSPVNSSNHGPVSTGSLTNRSPIEFPDTADFLNKPSVILQRSLGNAPNTPDFYQQLRNSDSNLCNSCGHQMLKYVSTSESDGTDCCSGKSGEEENIFSKESFKLMETQQEEETEKKDSRKLFQEGDKWLTEEAQSEQQTNIEQEVSLDLILVEEYDSLIEKMSNWNFPIFELVEKMGEKSGRILSQVMYTLFQDTGLLEIFKIPTQQFMNYFRALENGYRDIPYHNRIHATDVLHAVWYLTTRPVPGLQQIHNGCGTGNETDSDGRINHGRIAYISSKSCSNPDESYGCLSSNIPALELMALYVAAAMHDYDHPGRTNAFLVATNAPQAVLYNDRSVLENHHAASAWNLYLSRPEYNFLLHLDHVEFKRFRFLVIEAILATDLKKHFDFLAEFNAKANDVNSNGIEWSNENDRLLVCQVCIKLADINGPAKVRDLHLKWTEGIVNEFYEQGDEEANLGLPISPFMDRSSPQLAKLQESFITHIVGPLCNSYDAAGLLPGQWLEAEEDNDTESGDDEDGEELDTEDEEMENNLNPKPPRRKSRRRIFCQLMHHLTENHKIWKEIVEEEEKCKADGNKLQVENSSLPQADEIQVIEEADEEE

种属预测

种属预测:

score>80的预测可信度较高，可尝试用于WB检测。*预测模型主要基于免疫原序列比对，结果仅作参考，不作为质保凭据。

Species
Results
Score
Rabbit
100
Pig
88
Horse
88
Bovine
88
Sheep
88
Dog
88
Xenopus
88
Chicken
63
Zebrafish
0
Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

翻译修饰 - Q13370 作为底物

Site PTM Type Enzyme
S22 Phosphorylation
S26 Phosphorylation
K33 Ubiquitination
S37 Phosphorylation
S65 Phosphorylation
S73 Phosphorylation P17612 (PRKACA)
S279 Phosphorylation
S280 Phosphorylation
K285 Ubiquitination
S295 Phosphorylation P31749 (AKT1)
S296 Phosphorylation P17612 (PRKACA)
S299 Phosphorylation
T303 Phosphorylation
S306 Phosphorylation
S310 Phosphorylation
K312 Ubiquitination
S318 Phosphorylation P17612 (PRKACA)
S323 Phosphorylation
K339 Ubiquitination
S371 Phosphorylation
S378 Phosphorylation
S379 Phosphorylation
S393 Phosphorylation
K419 Ubiquitination
K426 Ubiquitination
S432 Phosphorylation
T435 Phosphorylation
S442 Phosphorylation
T444 Phosphorylation
S445 Phosphorylation
T487 Phosphorylation
S495 Phosphorylation
S514 Phosphorylation
S518 Phosphorylation
K546 Ubiquitination
S554 Phosphorylation
T561 Phosphorylation
Y565 Phosphorylation
S571 Phosphorylation
S573 Phosphorylation
S578 Phosphorylation
K585 Ubiquitination
Y586 Phosphorylation
S588 Phosphorylation
K601 Ubiquitination
S602 Phosphorylation
K611 Ubiquitination
S613 Phosphorylation
K632 Ubiquitination
Y751 Phosphorylation
T769 Phosphorylation
T773 Phosphorylation
K943 Ubiquitination
S981 Phosphorylation
K1073 Ubiquitination
K1081 Ubiquitination

研究背景

功能:

Cyclic nucleotide phosphodiesterase with a dual-specificity for the second messengers cAMP and cGMP, which are key regulators of many important physiological processes. May play a role in fat metabolism. Regulates cAMP binding of RAPGEF3. Through simultaneous binding to RAPGEF3 and PIK3R6 assembles a signaling complex in which the PI3K gamma complex is activated by RAPGEF3 and which is involved in angiogenesis.

细胞定位:

Membrane>Multi-pass membrane protein.

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionSubcellular location
组织特异性:

Abundant in adipose tissues.

亚基结构:

Interacts with PIK3CG (By similarity). Interacts with RAPGEF3 and PIK3R6.

蛋白家族:

Belongs to the cyclic nucleotide phosphodiesterase family. PDE3 subfamily.

研究领域

· Environmental Information Processing > Signal transduction > cGMP-PKG signaling pathway.   (View pathway)

· Environmental Information Processing > Signal transduction > cAMP signaling pathway.   (View pathway)

· Environmental Information Processing > Signal transduction > Apelin signaling pathway.   (View pathway)

· Human Diseases > Substance dependence > Morphine addiction.

· Metabolism > Nucleotide metabolism > Purine metabolism.

· Organismal Systems > Endocrine system > Insulin signaling pathway.   (View pathway)

· Organismal Systems > Endocrine system > Progesterone-mediated oocyte maturation.

· Organismal Systems > Endocrine system > Glucagon signaling pathway.

· Organismal Systems > Endocrine system > Regulation of lipolysis in adipocytes.

· Organismal Systems > Endocrine system > Renin secretion.

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