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| 产品: | PDE5A 抗体 |
| 货号: | DF9373 |
| 描述: | Rabbit polyclonal antibody to PDE5A |
| 应用: | WB IHC |
| 反应: | Human, Mouse, Rat |
| 预测: | Pig, Bovine, Horse, Sheep, Rabbit, Dog, Chicken |
| 分子量: | 100 kDa; 100kD(Calculated). |
| 蛋白号: | O76074 |
| RRID: | AB_2842569 |
产品描述
*The optimal dilutions should be determined by the end user.
*Tips:
WB: 适用于变性蛋白样本的免疫印迹检测. IHC: 适用于组织样本的石蜡(IHC-p)或冰冻(IHC-f)切片样本的免疫组化/荧光检测. IF/ICC: 适用于细胞样本的荧光检测. ELISA(peptide): 适用于抗原肽的ELISA检测.
引用格式: Affinity Biosciences Cat# DF9373, RRID:AB_2842569.
展开/折叠
5''-cyclic phosphodiesterase; CGB PDE; CGB-PDE; CGBPDE; cGMP binding cGMP specific 3' 5' cyclic nucleotide phosphodiesterase; cGMP binding cGMP specific 35 cyclic nucleotide phosphodiesterase; cGMP binding cGMP specific phosphodiesterase; cGMP binding/cGMP specific phosphodiesterase; cGMP specific 3'5' cyclic phosphodiesterase; cGMP specific phosphodiesterase PDE5A2; cGMP specific phosphodiesterase type 5A; cGMP-binding cGMP-specific phosphodiesterase; cGMP-specific 3''; CN5A; CN5N; PDE 5; PDE 5A; PDE5; Pde5a; PDE5A_HUMAN; PDE5A1; Phosphodiesterase 5A; Phosphodiesterase 5A cGMP specific; Phosphodiesterase isozyme 5;
抗原和靶标
Expressed in aortic smooth muscle cells, heart, placenta, skeletal muscle and pancreas and, to a much lesser extent, in brain, liver and lung.
- O76074 PDE5A_HUMAN:
- Protein BLAST With
- NCBI/
- ExPASy/
- Uniprot
MERAGPSFGQQRQQQQPQQQKQQQRDQDSVEAWLDDHWDFTFSYFVRKATREMVNAWFAERVHTIPVCKEGIRGHTESCSCPLQQSPRADNSAPGTPTRKISASEFDRPLRPIVVKDSEGTVSFLSDSEKKEQMPLTPPRFDHDEGDQCSRLLELVKDISSHLDVTALCHKIFLHIHGLISADRYSLFLVCEDSSNDKFLISRLFDVAEGSTLEEVSNNCIRLEWNKGIVGHVAALGEPLNIKDAYEDPRFNAEVDQITGYKTQSILCMPIKNHREEVVGVAQAINKKSGNGGTFTEKDEKDFAAYLAFCGIVLHNAQLYETSLLENKRNQVLLDLASLIFEEQQSLEVILKKIAATIISFMQVQKCTIFIVDEDCSDSFSSVFHMECEELEKSSDTLTREHDANKINYMYAQYVKNTMEPLNIPDVSKDKRFPWTTENTGNVNQQCIRSLLCTPIKNGKKNKVIGVCQLVNKMEENTGKVKPFNRNDEQFLEAFVIFCGLGIQNTQMYEAVERAMAKQMVTLEVLSYHASAAEEETRELQSLAAAVVPSAQTLKITDFSFSDFELSDLETALCTIRMFTDLNLVQNFQMKHEVLCRWILSVKKNYRKNVAYHNWRHAFNTAQCMFAALKAGKIQNKLTDLEILALLIAALSHDLDHRGVNNSYIQRSEHPLAQLYCHSIMEHHHFDQCLMILNSPGNQILSGLSIEEYKTTLKIIKQAILATDLALYIKRRGEFFELIRKNQFNLEDPHQKELFLAMLMTACDLSAITKPWPIQQRIAELVATEFFDQGDRERKELNIEPTDLMNREKKNKIPSMQVGFIDAICLQLYEALTHVSEDCFPLLDGCRKNRQKWQALAEQQEKMLINGESGQAKRN
种属预测
score>80的预测可信度较高，可尝试用于WB检测。*预测模型主要基于免疫原序列比对，结果仅作参考，不作为质保凭据。
High(score>80) Medium(80>score>50) Low(score<50) No confidence
翻译修饰 - O76074 作为底物
| Site | PTM Type | Enzyme | Source |
|---|---|---|---|
| S86 | Phosphorylation | Uniprot | |
| S92 | Phosphorylation | Uniprot | |
| S102 | Phosphorylation | P17612 (PRKACA) , Q13976 (PRKG1) | Uniprot |
| S104 | Phosphorylation | Uniprot | |
| T137 | Phosphorylation | Uniprot | |
| K243 | Ubiquitination | Uniprot | |
| K633 | Ubiquitination | Uniprot | |
| K714 | Ubiquitination | Uniprot | |
| S869 | Phosphorylation | Uniprot |
研究背景
Plays a role in signal transduction by regulating the intracellular concentration of cyclic nucleotides. This phosphodiesterase catalyzes the specific hydrolysis of cGMP to 5'-GMP. Specifically regulates nitric-oxide-generated cGMP.
Phosphorylation is regulated by binding of cGMP to the two allosteric sites (By similarity). Phosphorylation by PRKG1 leads to its activation.
Expressed in aortic smooth muscle cells, heart, placenta, skeletal muscle and pancreas and, to a much lesser extent, in brain, liver and lung.
Composed of a C-terminal catalytic domain containing two putative divalent metal sites and an N-terminal regulatory domain which contains two homologous allosteric cGMP-binding regions, A and B.
Belongs to the cyclic nucleotide phosphodiesterase family.
研究领域
· Environmental Information Processing > Signal transduction > cGMP-PKG signaling pathway. (View pathway)
· Metabolism > Nucleotide metabolism > Purine metabolism.
文献引用
Application: IHC Species: rat Sample: flap
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