产品描述
*The optimal dilutions should be determined by the end user.
*Tips:
WB: 适用于变性蛋白样本的免疫印迹检测. IHC: 适用于组织样本的石蜡(IHC-p)或冰冻(IHC-f)切片样本的免疫组化/荧光检测. IF/ICC: 适用于细胞样本的荧光检测. ELISA(peptide): 适用于抗原肽的ELISA检测.
引用格式: Affinity Biosciences Cat# DF9400, RRID:AB_2842596.
展开/折叠
Coagulation factor XI; Coagulation factor XIa light chain; F11; FA11_HUMAN; FXI; MGC141891; Plasma thromboplastin antecedent; PTA;
抗原和靶标
Isoform 2 is produced by platelets and megakaryocytes but absent from other blood cells.
- P03951 FA11_HUMAN:
- Protein BLAST With
- NCBI/
- ExPASy/
- Uniprot
MIFLYQVVHFILFTSVSGECVTQLLKDTCFEGGDITTVFTPSAKYCQVVCTYHPRCLLFTFTAESPSEDPTRWFTCVLKDSVTETLPRVNRTAAISGYSFKQCSHQISACNKDIYVDLDMKGINYNSSVAKSAQECQERCTDDVHCHFFTYATRQFPSLEHRNICLLKHTQTGTPTRITKLDKVVSGFSLKSCALSNLACIRDIFPNTVFADSNIDSVMAPDAFVCGRICTHHPGCLFFTFFSQEWPKESQRNLCLLKTSESGLPSTRIKKSKALSGFSLQSCRHSIPVFCHSSFYHDTDFLGEELDIVAAKSHEACQKLCTNAVRCQFFTYTPAQASCNEGKGKCYLKLSSNGSPTKILHGRGGISGYTLRLCKMDNECTTKIKPRIVGGTASVRGEWPWQVTLHTTSPTQRHLCGGSIIGNQWILTAAHCFYGVESPKILRVYSGILNQSEIKEDTSFFGVQEIIIHDQYKMAESGYDIALLKLETTVNYTDSQRPICLPSKGDRNVIYTDCWVTGWGYRKLRDKIQNTLQKAKIPLVTNEECQKRYRGHKITHKMICAGYREGGKDACKGDSGGPLSCKHNEVWHLVGITSWGEGCAQRERPGVYTNVVEYVDWILEKTQAV
种属预测
score>80的预测可信度较高，可尝试用于WB检测。*预测模型主要基于免疫原序列比对，结果仅作参考，不作为质保凭据。
High(score>80) Medium(80>score>50) Low(score<50) No confidence
翻译修饰 - P03951 作为底物
| Site | PTM Type | Enzyme | Source |
|---|---|---|---|
| S17 | Phosphorylation | Uniprot | |
| T22 | Phosphorylation | Uniprot | |
| N90 | N-Glycosylation | Uniprot | |
| Y115 | Phosphorylation | Uniprot | |
| Y125 | Phosphorylation | Uniprot | |
| N126 | N-Glycosylation | Uniprot | |
| S128 | Phosphorylation | Uniprot | |
| N163 | N-Glycosylation | Uniprot | |
| K180 | Acetylation | Uniprot | |
| S186 | Phosphorylation | Uniprot | |
| S394 | Phosphorylation | Uniprot | |
| N450 | N-Glycosylation | Uniprot | |
| N491 | N-Glycosylation | Uniprot | |
| T517 | Phosphorylation | Uniprot | |
| Y521 | Phosphorylation | Uniprot | |
| Y608 | Phosphorylation | Uniprot |
研究背景
Factor XI triggers the middle phase of the intrinsic pathway of blood coagulation by activating factor IX.
N-glycosylated on both chains. N-glycosylated sites mainly consist of nonfucosylated sialylated biantennary (in high abundance) and/or triantennary (in low abundance) complex structures. Glycosylation at Asn-163 uses a rare non-canonical Asn-X-Cys glycosite.
Activated by factor XIIa (or XII), which cleaves each polypeptide after Arg-387 into the light chain, which contains the active site, and the heavy chain, which associates with high molecular weight (HMW) kininogen.
Secreted.
Isoform 2 is produced by platelets and megakaryocytes but absent from other blood cells.
Homodimer; disulfide-linked. Forms a heterodimer with SERPINA5. After activation the heavy and light chains are also linked by a disulfide bond.
Belongs to the peptidase S1 family. Plasma kallikrein subfamily.
研究领域
· Organismal Systems > Immune system > Complement and coagulation cascades. (View pathway)
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