产品描述
*The optimal dilutions should be determined by the end user.
*Tips:
WB: 适用于变性蛋白样本的免疫印迹检测. IHC: 适用于组织样本的石蜡(IHC-p)或冰冻(IHC-f)切片样本的免疫组化/荧光检测. IF/ICC: 适用于细胞样本的荧光检测. ELISA(peptide): 适用于抗原肽的ELISA检测.
引用格式: Affinity Biosciences Cat# DF9428, RRID:AB_2842624.
展开/折叠
0610010E05Rik; C76600; Carnosinase , tissue; Carnosinase 2; CN 2; CN2; CNDP 2; CNDP dipeptidase 2 (metallopeptidase M20 family); CNDP dipeptidase 2; CNDP2; CNDP2_HUMAN; CPGL; Cytosolic non specific dipeptidase; Cytosolic non-specific dipeptidase; Cytosolic nonspecific dipeptidase; Dip-2; FLJ10830; FLJ35544; Glutamate carboxypeptidase like protein 1; Glutamate carboxypeptidase-like protein 1; HsT2298; Nonspecific dipeptidase, cutosolic; Pep-1; Pep1; PEPA; Peptidase A;
抗原和靶标
Isoform 1 is ubiquitously expressed with higher levels in kidney and liver (at protein level). Expressed in peripheral blood leukocytes (PubMed:12473676). Isoform 2 is expressed in fetal tissues, it is only expressed in adult liver and placental tissues. Down-regulated in gastric cancer tissues (at protein level) (PubMed:24395568).
- Q96KP4 CNDP2_HUMAN:
- Protein BLAST With
- NCBI/
- ExPASy/
- Uniprot
MAALTTLFKYIDENQDRYIKKLAKWVAIQSVSAWPEKRGEIRRMMEVAAADVKQLGGSVELVDIGKQKLPDGSEIPLPPILLGRLGSDPQKKTVCIYGHLDVQPAALEDGWDSEPFTLVERDGKLYGRGSTDDKGPVAGWINALEAYQKTGQEIPVNVRFCLEGMEESGSEGLDELIFARKDTFFKDVDYVCISDNYWLGKKKPCITYGLRGICYFFIEVECSNKDLHSGVYGGSVHEAMTDLILLMGSLVDKRGNILIPGINEAVAAVTEEEHKLYDDIDFDIEEFAKDVGAQILLHSHKKDILMHRWRYPSLSLHGIEGAFSGSGAKTVIPRKVVGKFSIRLVPNMTPEVVGEQVTSYLTKKFAELRSPNEFKVYMGHGGKPWVSDFSHPHYLAGRRAMKTVFGVEPDLTREGGSIPVTLTFQEATGKNVMLLPVGSADDGAHSQNEKLNRYNYIEGTKMLAAYLYEVSQLKD
种属预测
score>80的预测可信度较高，可尝试用于WB检测。*预测模型主要基于免疫原序列比对，结果仅作参考，不作为质保凭据。
High(score>80) Medium(80>score>50) Low(score<50) No confidence
翻译修饰 - Q96KP4 作为底物
| Site | PTM Type | Enzyme | Source |
|---|---|---|---|
| A2 | Acetylation | Uniprot | |
| T6 | Phosphorylation | Uniprot | |
| K9 | Acetylation | Uniprot | |
| R17 | Methylation | Uniprot | |
| K20 | Ubiquitination | Uniprot | |
| K24 | Ubiquitination | Uniprot | |
| K53 | Ubiquitination | Uniprot | |
| S58 | Phosphorylation | Uniprot | |
| K66 | Ubiquitination | Uniprot | |
| K68 | Ubiquitination | Uniprot | |
| S87 | Phosphorylation | Uniprot | |
| K149 | Ubiquitination | Uniprot | |
| Y190 | Phosphorylation | Uniprot | |
| K202 | Ubiquitination | Uniprot | |
| K203 | Ubiquitination | Uniprot | |
| T207 | Phosphorylation | Uniprot | |
| Y232 | Phosphorylation | Uniprot | |
| Y277 | Phosphorylation | Uniprot | |
| K289 | Ubiquitination | Uniprot | |
| S299 | Phosphorylation | Uniprot | |
| K301 | Ubiquitination | Uniprot | |
| K302 | Ubiquitination | Uniprot | |
| Y311 | Phosphorylation | Uniprot | |
| K339 | Ubiquitination | Uniprot | |
| K383 | Ubiquitination | Uniprot | |
| K402 | Ubiquitination | Uniprot | |
| K430 | Ubiquitination | Uniprot | |
| S439 | Phosphorylation | Uniprot | |
| S446 | Phosphorylation | Uniprot | |
| K450 | Ubiquitination | Uniprot |
研究背景
Hydrolyzes a variety of dipeptides including L-carnosine but has a strong preference for Cys-Gly. Acts as a functional tumor suppressor in gastric cancer via activation of the mitogen-activated protein kinase (MAPK) pathway. An elevated level of CNDP2 activates the p38 and JNK MAPK pathways to induce cell apoptosis, and a lower level of CNDP2 activates the ERK MAPK pathway to promote cell proliferation. Isoform 2 may play a role as tumor suppressor in hepatocellular carcinoma (HCC) cells. Catalyzes the production of N-lactoyl-amino acids from lactate and amino acids by reverse proteolysis.
Cytoplasm.
Isoform 1 is ubiquitously expressed with higher levels in kidney and liver (at protein level). Expressed in peripheral blood leukocytes. Isoform 2 is expressed in fetal tissues, it is only expressed in adult liver and placental tissues. Down-regulated in gastric cancer tissues (at protein level).
Homodimer.
Belongs to the peptidase M20A family.
研究领域
· Metabolism > Amino acid metabolism > Arginine and proline metabolism.
· Metabolism > Amino acid metabolism > Histidine metabolism.
· Metabolism > Metabolism of other amino acids > beta-Alanine metabolism.
· Metabolism > Global and overview maps > Metabolic pathways.
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