| 产品: | FANCL 抗体 |
| 货号: | DF9476 |
| 描述: | Rabbit polyclonal antibody to FANCL |
| 应用: | WB |
| 反应: | Human, Mouse, Rat |
| 预测: | Pig, Bovine, Horse, Sheep, Rabbit, Dog, Chicken, Xenopus |
| 分子量: | 43 kDa; 43kD(Calculated). |
| 蛋白号: | Q9NW38 |
| RRID: | AB_2842672 |
产品描述
*The optimal dilutions should be determined by the end user.
*Tips:
WB: 适用于变性蛋白样本的免疫印迹检测. IHC: 适用于组织样本的石蜡(IHC-p)或冰冻(IHC-f)切片样本的免疫组化/荧光检测. IF/ICC: 适用于细胞样本的荧光检测. ELISA(peptide): 适用于抗原肽的ELISA检测.
引用格式: Affinity Biosciences Cat# DF9476, RRID:AB_2842672.
展开/折叠
E3 ubiquitin-protein ligase FANCL; FAAP43; Fancl; FANCL_HUMAN; Fanconi anemia complementation group L; Fanconi anemia group L protein; Fanconi anemia-associated polypeptide of 43 kDa; FLJ10335; PHD finger protein 9; PHF9; POG; Ubiquitin ligase protein FANCL;
抗原和靶标
- Q9NW38 FANCL_HUMAN:
- Protein BLAST With
- NCBI/
- ExPASy/
- Uniprot
MAVTEASLLRQCPLLLPQNRSKTVYEGFISAQGRDFHLRIVLPEDLQLKNARLLCSWQLRTILSGYHRIVQQRMQHSPDLMSFMMELKMLLEVALKNRQELYALPPPPQFYSSLIEEIGTLGWDKLVYADTCFSTIKLKAEDASGREHLITLKLKAKYPAESPDYFVDFPVPFCASWTPQSSLISIYSQFLAAIESLKAFWDVMDEIDEKTWVLEPEKPPRSATARRIALGNNVSINIEVDPRHPTMLPECFFLGADHVVKPLGIKLSRNIHLWDPENSVLQNLKDVLEIDFPARAILEKSDFTMDCGICYAYQLDGTIPDQVCDNSQCGQPFHQICLYEWLRGLLTSRQSFNIIFGECPYCSKPITLKMSGRKH
种属预测
score>80的预测可信度较高，可尝试用于WB检测。*预测模型主要基于免疫原序列比对，结果仅作参考，不作为质保凭据。
High(score>80) Medium(80>score>50) Low(score<50) No confidence
翻译修饰 - Q9NW38 作为底物
| Site | PTM Type | Enzyme | Source |
|---|---|---|---|
| A2 | Acetylation | Uniprot | |
| T4 | Phosphorylation | Uniprot | |
| S7 | Phosphorylation | Uniprot | |
| K22 | Ubiquitination | Uniprot | |
| K49 | Ubiquitination | Uniprot | |
| K139 | Sumoylation | Uniprot | |
| K153 | Ubiquitination | Uniprot | |
| T178 | Phosphorylation | Uniprot | |
| K218 | Ubiquitination | Uniprot | |
| K261 | Ubiquitination | Uniprot | |
| K266 | Ubiquitination | Uniprot | |
| S351 | Phosphorylation | Uniprot | |
| K364 | Ubiquitination | Uniprot |
研究背景
Ubiquitin ligase protein that mediates monoubiquitination of FANCD2 in the presence of UBE2T, a key step in the DNA damage pathway. Also mediates monoubiquitination of FANCI. May stimulate the ubiquitin release from UBE2W. May be required for proper primordial germ cell proliferation in the embryonic stage, whereas it is probably not needed for spermatogonial proliferation after birth.
The RING-type zinc finger domain is monoubiquitinated in the presence of UBE2T and UBE2W.
Cytoplasm. Nucleus.
Interacts with GGN (By similarity). Belongs to the multisubunit FA complex composed of FANCA, FANCB, FANCC, FANCE, FANCF, FANCG, FANCL/PHF9 and FANCM. The complex is not found in FA patients. In complex with FANCF, FANCA and FANCG, but not with FANCC, nor FANCE, interacts with HES1; this interaction may be essential for the stability and nuclear localization of FA core complex proteins. Interacts with FANCI. Directly interacts (via the RING-type zinc finger) with UBE2T and UBE2W.
The UBC-RWD region (URD) region mediates interaction with FANCI and FANCD2.
研究领域
· Genetic Information Processing > Replication and repair > Fanconi anemia pathway.
· Genetic Information Processing > Folding, sorting and degradation > Ubiquitin mediated proteolysis. (View pathway)
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