| 产品: | LRSAM1 抗体 |
| 货号: | DF9480 |
| 描述: | Rabbit polyclonal antibody to LRSAM1 |
| 应用: | WB IHC |
| 反应: | Human, Mouse, Rat |
| 预测: | Pig, Zebrafish, Bovine, Horse, Sheep, Dog, Chicken, Xenopus |
| 分子量: | 84 kDa; 84kD(Calculated). |
| 蛋白号: | Q6UWE0 |
| RRID: | AB_2842676 |
产品描述
*The optimal dilutions should be determined by the end user.
*Tips:
WB: 适用于变性蛋白样本的免疫印迹检测. IHC: 适用于组织样本的石蜡(IHC-p)或冰冻(IHC-f)切片样本的免疫组化/荧光检测. IF/ICC: 适用于细胞样本的荧光检测. ELISA(peptide): 适用于抗原肽的ELISA检测.
引用格式: Affinity Biosciences Cat# DF9480, RRID:AB_2842676.
展开/折叠
CMT2P; E3 ubiquitin protein ligase LRSAM1; E3 ubiquitin-protein ligase LRSAM1; EC 6.3.2.-; FLJ31641; hTAL; Leucine rich repeat and sterile alpha motif containing 1; Leucine rich repeat and sterile alpha motif containing protein 1; Leucine-rich repeat and sterile alpha motif-containing protein 1; LRSAM1; LRSM1_HUMAN; OTTHUMP00000022174; OTTHUMP00000022175; RIFLE; TAL; Tsg101 associated ligase; Tsg101-associated ligase;
抗原和靶标
Highly expressed in adult spinal cord motoneurons as well as in fetal spinal cord and muscle tissue.
- Q6UWE0 LRSM1_HUMAN:
- Protein BLAST With
- NCBI/
- ExPASy/
- Uniprot
MPLFFRKRKPSEEARKRLEYQMCLAKEAGADDILDISKCELSEIPFGAFATCKVLQKKVLIVHTNHLTSLLPKSCSLLSLATIKVLDLHDNQLTALPDDLGQLTALQVLNVERNQLMQLPRSIGNLTQLQTLNVKDNKLKELPDTVGELRSLRTLNISGNEIQRLPQMLAHVRTLEMLSLDASAMVYPPREVCGAGTAAILQFLCKESGLEYYPPSQYLLPILEQDGIENSRDSPDGPTDRFSREELEWQNRFSDYEKRKEQKMLEKLEFERRLELGQREHTQLLQQSSSQKDEILQTVKEEQSRLEQGLSEHQRHLNAERQRLQEQLKQTEQNISSRIQKLLQDNQRQKKSSEILKSLENERIRMEQLMSITQEETESLRRRDVASAMQQMLTESCKNRLIQMAYESQRQNLVQQACSSMAEMDERFQQILSWQQMDQNKAISQILQESAMQKAAFEALQVKKDLMHRQIRSQIKLIETELLQLTQLELKRKSLDTESLQEMISEQRWALSSLLQQLLKEKQQREEELREILTELEAKSETRQENYWLIQYQRLLNQKPLSLKLQEEGMERQLVALLEELSAEHYLPIFAHHRLSLDLLSQMSPGDLAKVGVSEAGLQHEILRRVQELLDAARIQPELKPPMGEVVTPTAPQEPPESVRPSAPPAELEVQASECVVCLEREAQMIFLNCGHVCCCQQCCQPLRTCPLCRQDIAQRLRIYHSS
种属预测
score>80的预测可信度较高，可尝试用于WB检测。*预测模型主要基于免疫原序列比对，结果仅作参考，不作为质保凭据。
High(score>80) Medium(80>score>50) Low(score<50) No confidence
翻译修饰 - Q6UWE0 作为底物
| Site | PTM Type | Enzyme | Source |
|---|---|---|---|
| K9 | Ubiquitination | Uniprot | |
| K38 | Ubiquitination | Uniprot | |
| S79 | Phosphorylation | Uniprot | |
| S179 | Phosphorylation | Uniprot | |
| Y187 | Phosphorylation | Uniprot | |
| Y218 | Phosphorylation | Uniprot | |
| S234 | Phosphorylation | Uniprot | |
| Y256 | Phosphorylation | Uniprot | |
| T282 | Phosphorylation | Uniprot | |
| S289 | Phosphorylation | Uniprot | |
| S290 | Phosphorylation | Uniprot | |
| T298 | Phosphorylation | Uniprot | |
| K329 | Ubiquitination | Uniprot | |
| S337 | Phosphorylation | Uniprot | |
| K341 | Ubiquitination | Uniprot | |
| K357 | Ubiquitination | Uniprot | |
| K491 | Ubiquitination | Uniprot | |
| S494 | Phosphorylation | Uniprot | |
| S499 | Phosphorylation | Uniprot | |
| K520 | Ubiquitination | Uniprot | |
| K522 | Ubiquitination | Uniprot | |
| T534 | Phosphorylation | Uniprot | |
| S562 | Phosphorylation | Uniprot | |
| K564 | Ubiquitination | Uniprot | |
| S596 | Phosphorylation | Uniprot | |
| S604 | Phosphorylation | Uniprot |
研究背景
E3 ubiquitin-protein ligase that mediates monoubiquitination of TSG101 at multiple sites, leading to inactivate the ability of TSG101 to sort endocytic (EGF receptors) and exocytic (HIV-1 viral proteins) cargos. Bacterial recognition protein that defends the cytoplasm from invasive pathogens. Localizes to several intracellular bacterial pathogens and generates the bacteria-associated ubiquitin signal leading to autophagy-mediated intracellular bacteria degradation (xenophagy).
Ubiquitination promoted by PHF23 leads to proteasomal degradation.
Cytoplasm.
Note: Displays a punctuate distribution and localizes to a submembranal ring (PubMed:15256501). Localizes to intracellular bacterial pathogens (PubMed:23245322).
Highly expressed in adult spinal cord motoneurons as well as in fetal spinal cord and muscle tissue.
Interacts with TSG101. Interacts with PHF23. Interacts with FUS.
The coiled coil domains interact with the SB domain of TSG101.
The PTAP motifs mediate the binding to UEV domains.
The LRR domain is necessary and sufficient for localization to bacterial targets.
The RING domain is required for ubiquitination.
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