UBR2 Antibody - #DF9491

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产品: UBR2 抗体
货号: DF9491
描述: Rabbit polyclonal antibody to UBR2
应用: WB IHC
文献验证: WB
反应: Human, Mouse
预测: Pig, Zebrafish, Bovine, Horse, Sheep, Rabbit, Dog, Chicken, Xenopus
分子量: 201kDa; 201kD(Calculated).
蛋白号: Q8IWV8
RRID: AB_2842687

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   规格 价格 库存
 100ul RMB¥ 2300 现货
 200ul RMB¥ 3000 现货

货期: 当天发货

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MS Report
HPLC Report
MSDS
说明书
COA
实验方案
WB Handbook
IHC Protocol
IF/ICC Protocol

产品描述

来源:
Rabbit
应用:
WB 1:1000-3000, IHC 1:50-1:200
*The optimal dilutions should be determined by the end user.
*Tips:

WB: 适用于变性蛋白样本的免疫印迹检测. IHC: 适用于组织样本的石蜡(IHC-p)或冰冻(IHC-f)切片样本的免疫组化/荧光检测. IF/ICC: 适用于细胞样本的荧光检测. ELISA(peptide): 适用于抗原肽的ELISA检测.

反应:
Human,Mouse
预测:
Pig(100%), Zebrafish(100%), Bovine(100%), Horse(100%), Sheep(100%), Rabbit(100%), Dog(100%), Chicken(100%), Xenopus(100%)
克隆:
Polyclonal
特异性:
UBR2 Antibody detects endogenous levels of total UBR2.
RRID:
AB_2842687
引用格式: Affinity Biosciences Cat# DF9491, RRID:AB_2842687.
偶联:
Unconjugated.
纯化:
The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).
保存:
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
别名:

展开/折叠

bA49A4.1; C6orf133; dJ242G1.1; dJ392M17.3; DKFZp686C08114; E3 ubiquitin protein ligase UBR2; KIAA0349; MGC71112; N recognin 2; OTTHUMP00000016403; OTTHUMP00000039767; OTTHUMP00000039768; RP3 392M17.3; Ubiquitin ligase E3 alpha II; Ubiquitin protein ligase E3 alpha 2; Ubiquitin protein ligase E3 alpha II; Ubiquitin protein ligase E3 component n recognin 2; UBR 2;

抗原和靶标

免疫原:
Uniprot:

Q8IWV8(UBR2_HUMAN) >>Visit HPA database.

基因/基因ID:
UBR2(23304)
表达:
Q8IWV8 UBR2_HUMAN:

Broadly expressed, with highest levels in skeletal muscle, kidney and pancreas. Present in acinar cells of the pancreas (at protein level).

序列:
MASELEPEVQAIDRSLLECSAEEIAGKWLQATDLTREVYQHLAHYVPKIYCRGPNPFPQKEDMLAQHVLLGPMEWYLCGEDPAFGFPKLEQANKPSHLCGRVFKVGEPTYSCRDCAVDPTCVLCMECFLGSIHRDHRYRMTTSGGGGFCDCGDTEAWKEGPYCQKHELNTSEIEEEEDPLVHLSEDVIARTYNIFAITFRYAVEILTWEKESELPADLEMVEKSDTYYCMLFNDEVHTYEQVIYTLQKAVNCTQKEAIGFATTVDRDGRRSVRYGDFQYCEQAKSVIVRNTSRQTKPLKVQVMHSSIVAHQNFGLKLLSWLGSIIGYSDGLRRILCQVGLQEGPDGENSSLVDRLMLSDSKLWKGARSVYHQLFMSSLLMDLKYKKLFAVRFAKNYQQLQRDFMEDDHERAVSVTALSVQFFTAPTLARMLITEENLMSIIIKTFMDHLRHRDAQGRFQFERYTALQAFKFRRVQSLILDLKYVLISKPTEWSDELRQKFLEGFDAFLELLKCMQGMDPITRQVGQHIEMEPEWEAAFTLQMKLTHVISMMQDWCASDEKVLIEAYKKCLAVLMQCHGGYTDGEQPITLSICGHSVETIRYCVSQEKVSIHLPVSRLLAGLHVLLSKSEVAYKFPELLPLSELSPPMLIEHPLRCLVLCAQVHAGMWRRNGFSLVNQIYYYHNVKCRREMFDKDVVMLQTGVSMMDPNHFLMIMLSRFELYQIFSTPDYGKRFSSEITHKDVVQQNNTLIEEMLYLIIMLVGERFSPGVGQVNATDEIKREIIHQLSIKPMAHSELVKSLPEDENKETGMESVIEAVAHFKKPGLTGRGMYELKPECAKEFNLYFYHFSRAEQSKAEEAQRKLKRQNREDTALPPPVLPPFCPLFASLVNILQSDVMLCIMGTILQWAVEHNGYAWSESMLQRVLHLIGMALQEEKQHLENVTEEHVVTFTFTQKISKPGEAPKNSPSILAMLETLQNAPYLEVHKDMIRWILKTFNAVKKMRESSPTSPVAETEGTIMEESSRDKDKAERKRKAEIARLRREKIMAQMSEMQRHFIDENKELFQQTLELDASTSAVLDHSPVASDMTLTALGPAQTQVPEQRQFVTCILCQEEQEVKVESRAMVLAAFVQRSTVLSKNRSKFIQDPEKYDPLFMHPDLSCGTHTSSCGHIMHAHCWQRYFDSVQAKEQRRQQRLRLHTSYDVENGEFLCPLCECLSNTVIPLLLPPRNIFNNRLNFSDQPNLTQWIRTISQQIKALQFLRKEESTPNNASTKNSENVDELQLPEGFRPDFRPKIPYSESIKEMLTTFGTATYKVGLKVHPNEEDPRVPIMCWGSCAYTIQSIERILSDEDKPLFGPLPCRLDDCLRSLTRFAAAHWTVASVSVVQGHFCKLFASLVPNDSHEELPCILDIDMFHLLVGLVLAFPALQCQDFSGISLGTGDLHIFHLVTMAHIIQILLTSCTEENGMDQENPPCEEESAVLALYKTLHQYTGSALKEIPSGWHLWRSVRAGIMPFLKCSALFFHYLNGVPSPPDIQVPGTSHFEHLCSYLSLPNNLICLFQENSEIMNSLIESWCRNSEVKRYLEGERDAIRYPRESNKLINLPEDYSSLINQASNFSCPKSGGDKSRAPTLCLVCGSLLCSQSYCCQTELEGEDVGACTAHTYSCGSGVGIFLRVRECQVLFLAGKTKGCFYSPPYLDDYGETDQGLRRGNPLHLCKERFKKIQKLWHQHSVTEEIGHAQEANQTLVGIDWQHL

种属预测

种属预测:

score>80的预测可信度较高，可尝试用于WB检测。*预测模型主要基于免疫原序列比对，结果仅作参考，不作为质保凭据。

Species
Results
Score
Pig
100
Horse
100
Bovine
100
Sheep
100
Dog
100
Xenopus
100
Zebrafish
100
Chicken
100
Rabbit
100
Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

翻译修饰 - Q8IWV8 作为底物

Site PTM Type Enzyme
A2 Acetylation
K27 Ubiquitination
K48 Ubiquitination
K94 Ubiquitination
K104 Ubiquitination
Y110 Phosphorylation
T141 Phosphorylation
T142 Phosphorylation
S143 Phosphorylation
T154 Phosphorylation
S224 Phosphorylation
K255 Ubiquitination
Y274 Phosphorylation
K284 Ubiquitination
S328 Phosphorylation
K443 Ubiquitination
T581 Phosphorylation
S734 Phosphorylation
K779 Ubiquitination
K789 Ubiquitination
K798 Ubiquitination
K821 Ubiquitination
K822 Ubiquitination
T826 Phosphorylation
Y844 Phosphorylation
K994 Ubiquitination
S1005 Phosphorylation
S1006 Phosphorylation
T1008 Phosphorylation
S1009 Phosphorylation
T1014 Phosphorylation
T1017 Phosphorylation
S1022 Phosphorylation
K1044 Ubiquitination
S1050 Phosphorylation
K1142 Ubiquitination
K1187 Ubiquitination
K1255 Ubiquitination
T1266 Phosphorylation
K1273 Ubiquitination
K1294 Ubiquitination
T1312 Phosphorylation
K1599 Ubiquitination
Y1607 Phosphorylation
K1621 Ubiquitination
K1689 Ubiquitination
K1718 Ubiquitination

研究背景

功能:

E3 ubiquitin-protein ligase which is a component of the N-end rule pathway. Recognizes and binds to proteins bearing specific N-terminal residues that are destabilizing according to the N-end rule, leading to their ubiquitination and subsequent degradation. Plays a critical role in chromatin inactivation and chromosome-wide transcriptional silencing during meiosis via ubiquitination of histone H2A. Binds leucine and is a negative regulator of the leucine-mTOR signaling pathway, thereby controlling cell growth. Required for spermatogenesis, promotes, with Tex19.1, SPO11-dependent recombination foci to accumulate and drive robust homologous chromosome synapsis (By similarity). Polyubiquitinates LINE-1 retrotransposon encoded, LIRE1, which induces degradation, inhibiting LINE-1 retrotransposon mobilization (By similarity).

细胞定位:

Nucleus.
Note: Associated with chromatin during meiosis.

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionSubcellular location
组织特异性:

Broadly expressed, with highest levels in skeletal muscle, kidney and pancreas. Present in acinar cells of the pancreas (at protein level).

亚基结构:

Interacts with UBE2B; promotes the UBE2B-H2A interaction and the ubiquitination of histone H2A by UBE2B and UBR2 (By similarity). Interacts with RECQL4. Interacts with TEX19; does not lead to TEX19 degradation and stabilizes it (By similarity). Interacts with CASP8. Interacts with ATXN3.

蛋白家族:

The RING-H2 zinc finger is an atypical RING finger with a His ligand in place of the fourth Cys of the classical motif.

The UBR-type zinc finger forms a pocket that mediates recognition of type 1 N-degrons. It exhibits preference for Arginine in first position, has poor affinity for histidine, and doesn't bind acetylated peptides.

Belongs to the UBR1 family.

文献引用

1). Amelioration of hepatic steatosis by dietary essential amino acid-induced ubiquitination. Molecular cell, 2021 (PubMed: 35245436) [IF=14.5]

Application: WB    Species: Mouse    Sample:

Figure 3The physiological function of Ubr1 family proteins in preventing hepatic steatosis is evolutionarily conserved
2). Identification of Ubr1 as an amino acid sensor of steatosis in liver and muscle. Journal of Cachexia, Sarcopenia and Muscle, 2023 (PubMed: 37057345) [IF=9.4]

Application: WB    Species: Mouse    Sample:

Figure 6 Amino acid‐induced PLIN2 degradation is evolutionarily conserved. Endogenous mouse Ubr1/2 and human UBR1/2 specifically recognized endogenous Plin2/PLIN2 in an amino acid‐dependent manner (A–C). Knockdown of both Ubr1 and Ubr2 (abbreviated as Ubr1/2) led to increased protein levels of endogenous Plin2 in mouse AML12 normal hepatocytes (D). In contrast, knocking down either Ubr1 or Ubr2 alone had no effects (D). Knocking down both human UBR1 and UBR2 (abbreviated as UBR1/2), but neither alone, stimulated protein levels of endogenous PLIN2 in human JHH‐7 (E) and SNU‐475 hepatoma cells (F). For (A), (B), and (C), MG132 was added to prevent PLIN2 degradation in the proteasome.

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