EEF1A2 Antibody - #DF9493

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产品: EEF1A2 抗体
货号: DF9493
描述: Rabbit polyclonal antibody to EEF1A2
应用: WB IF/ICC
反应: Human, Mouse, Rat
预测: Pig, Bovine, Horse, Sheep, Rabbit, Chicken, Xenopus
分子量: 50 kDa; 50kD(Calculated).
蛋白号: Q05639
RRID: AB_2842689

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 100ul RMB¥ 2300 现货
 200ul RMB¥ 3000 现货

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IF/ICC Protocol

产品描述

来源:
Rabbit
应用:
WB 1:1000-3000, IF/ICC 1:100-1:500
*The optimal dilutions should be determined by the end user.
*Tips:

WB: 适用于变性蛋白样本的免疫印迹检测. IHC: 适用于组织样本的石蜡(IHC-p)或冰冻(IHC-f)切片样本的免疫组化/荧光检测. IF/ICC: 适用于细胞样本的荧光检测. ELISA(peptide): 适用于抗原肽的ELISA检测.

反应:
Human,Mouse,Rat
预测:
Pig(100%), Bovine(100%), Horse(100%), Sheep(100%), Rabbit(100%), Chicken(100%), Xenopus(91%)
克隆:
Polyclonal
特异性:
EEF1A2 Antibody detects endogenous levels of total EEF1A2.
RRID:
AB_2842689
引用格式: Affinity Biosciences Cat# DF9493, RRID:AB_2842689.
偶联:
Unconjugated.
纯化:
The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).
保存:
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
别名:

展开/折叠

CCS3; eEF1A-1; eEF1A-2; EEF1A1; Eef1a2; EEF1AL; EF 1 alpha 2; EF-1-alpha-1; EF-1-alpha-2; EF-Tu; EF1A; EF1A1_HUMAN; EF1A2_HUMAN; Elongation factor 1 A 2; Elongation factor 1 alpha; Elongation factor 1 alpha 2; Elongation factor 1-alpha 1; Elongation factor 1-alpha 2; Elongation factor Tu; Eukaryotic elongation factor 1 A-1; Eukaryotic elongation factor 1 A-2; Eukaryotic translation elongation factor 1 alpha 2; GRAF 1EF; HS1; Leukocyte receptor cluster member 7; PTI1; Statin-S1;

抗原和靶标

免疫原:
Uniprot:

Q05639(EF1A2_HUMAN) >>Visit HPA database.

基因/基因ID:
EEF1A2(1917)
表达:
Q05639 EF1A2_HUMAN:

Brain, heart, and skeletal muscle.

序列:
MGKEKTHINIVVIGHVDSGKSTTTGHLIYKCGGIDKRTIEKFEKEAAEMGKGSFKYAWVLDKLKAERERGITIDISLWKFETTKYYITIIDAPGHRDFIKNMITGTSQADCAVLIVAAGVGEFEAGISKNGQTREHALLAYTLGVKQLIVGVNKMDSTEPAYSEKRYDEIVKEVSAYIKKIGYNPATVPFVPISGWHGDNMLEPSPNMPWFKGWKVERKEGNASGVSLLEALDTILPPTRPTDKPLRLPLQDVYKIGGIGTVPVGRVETGILRPGMVVTFAPVNITTEVKSVEMHHEALSEALPGDNVGFNVKNVSVKDIRRGNVCGDSKSDPPQEAAQFTSQVIILNHPGQISAGYSPVIDCHTAHIACKFAELKEKIDRRSGKKLEDNPKSLKSGDAAIVEMVPGKPMCVESFSQYPPLGRFAVRDMRQTVAVGVIKNVEKKSGGAGKVTKSAQKAQKAGK

种属预测

种属预测:

score>80的预测可信度较高，可尝试用于WB检测。*预测模型主要基于免疫原序列比对，结果仅作参考，不作为质保凭据。

Species
Results
Score
Pig
100
Horse
100
Bovine
100
Sheep
100
Chicken
100
Rabbit
100
Xenopus
91
Zebrafish
60
Dog
0
Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

翻译修饰 - Q05639 作为底物

Site PTM Type Enzyme
S21 Phosphorylation P15056 (BRAF) , P04049 (RAF1)
T22 Phosphorylation
T23 Phosphorylation
T24 Phosphorylation
Y29 Phosphorylation
K30 Acetylation
K30 Methylation
K30 Ubiquitination
K36 Acetylation
K36 Methylation
K36 Ubiquitination
R37 Methylation
K41 Acetylation
K41 Sumoylation
K41 Ubiquitination
K44 Acetylation
K44 Ubiquitination
K51 Methylation
K51 Ubiquitination
S53 Phosphorylation P05771 (PRKCB)
K55 Acetylation
K55 Methylation
K55 Sumoylation
K55 Ubiquitination
Y56 Phosphorylation
K62 Methylation
K62 Ubiquitination
R69 Methylation
K79 Acetylation
K79 Methylation
K84 Methylation
Y85 Phosphorylation
Y86 Phosphorylation
T88 Phosphorylation
T104 Phosphorylation
T106 Phosphorylation
S128 Phosphorylation
K129 Ubiquitination
T133 Phosphorylation
R134 Methylation
Y141 Phosphorylation
T142 Phosphorylation
K146 Acetylation
K146 Ubiquitination
K154 Ubiquitination
S163 Phosphorylation
K165 Acetylation
K165 Methylation
K165 Ubiquitination
R166 Methylation
K172 Ubiquitination
S175 Phosphorylation
Y177 Phosphorylation
K179 Acetylation
S205 Phosphorylation P45983 (MAPK8)
K219 Ubiquitination
T234 Phosphorylation
T239 Phosphorylation
K244 Ubiquitination
Y254 Phosphorylation
K255 Acetylation
K255 Methylation
K255 Ubiquitination
T261 Phosphorylation
R266 Methylation
T269 Phosphorylation
T287 Phosphorylation
S291 Phosphorylation
S300 Phosphorylation
K313 Sumoylation
K313 Ubiquitination
K318 Acetylation
K318 Methylation
K318 Ubiquitination
S358 Phosphorylation P45983 (MAPK8)
S383 Phosphorylation
K392 Ubiquitination
S393 Phosphorylation
R430 Methylation
T432 Phosphorylation
K439 Acetylation
S445 Phosphorylation
T452 Phosphorylation
K453 Ubiquitination

研究背景

功能:

This protein promotes the GTP-dependent binding of aminoacyl-tRNA to the A-site of ribosomes during protein biosynthesis.

翻译修饰:

Trimethylated at Lys-165 by EEF1AKMT3. Mono-, di-, and trimethylated at Lys-36 by EEF1AKMT4; trimethylated form is predominant. Methylation by EEF1AKMT4 contributes to the fine-tuning of translation rates for a subset of tRNAs. Trimethylated at the N-terminus by EEF1AKNMT. Mono- and dimethylated at Lys-55 by EEF1AKNMT; dimethylated form is predominant.

细胞定位:

Nucleus.

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionSubcellular location
组织特异性:

Brain, heart, and skeletal muscle.

亚基结构:

Monomer.

蛋白家族:

Belongs to the TRAFAC class translation factor GTPase superfamily. Classic translation factor GTPase family. EF-Tu/EF-1A subfamily.

研究领域

· Genetic Information Processing > Translation > RNA transport.

· Human Diseases > Infectious diseases: Bacterial > Legionellosis.

文献引用

1). EEF1A2 interacts with HSP90AB1 to promote lung adenocarcinoma metastasis via enhancing TGF-β/SMAD signalling. BRITISH JOURNAL OF CANCER (PubMed: 33473168) [IF=8.8]

Application: WB    Species: human    Sample: A549 and PC9 cells

Fig. 6| EEF1A2 regulates TGF-β/SMAD signalling based on HSP90AB1. a HSP90AB1 silencing inhibited the invasion potential of A549 cells induced by EEF1A2 overexpression. Data are shown as mean ± SEM; n = 3 independent experiments, two-tailed Student’s t-test. b A549 and PC9 cells were co-transfected with PCDH-EEF1A2, and si-HSP90AB1. The protein expression levels of HSP90AB1, EEF1A1, TβRI, TβRII, SMAD3,pSMAD3, N-cadherin and SLUG were detected using western blot analysis.

Application: IF/ICC    Species: human    Sample: A549 cells

Supplementary Figure 3.| EEF1A2 interacts with HSP90AB1. (D) Confocal images shown position of co-location EEF1A2 (green) and HSP90AB1 (red) after transfection of si-EEF1A2 or si-HSP90AB1 in A549 cells. Images were acquired at 400×. Scale bar = 50 µm.
2). Biliverdin modulates the Nrf2/A20/eEF1A2 axis to alleviate cerebral ischemia-reperfusion injury by inhibiting pyroptosis. Biomedicine & Pharmacotherapy (PubMed: 37399716) [IF=7.5]

Application: WB    Species: Mouse    Sample:

Fig. 8. The interaction of A20 with eEF1A2. A. Mass spectrum data of eEF1A2 among A20-interacting proteins identified by mass spectrometry. B. Co-IP assay confirmed the interaction between A20 and eEF1A2, with or without MG-132; C. eEF1A2 mRNA expression; D. eEF1A2 protein expression; E. Western blot images of eEF1A2 and GAPDH.
3). Cyclin-dependent kinase subunit2 (CKS2) promotes malignant phenotypes and epithelial-mesenchymal transition-like process in glioma by activating TGFβ/SMAD signaling. Cancer Medicine (PubMed: 36284444) [IF=4.0]

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