产品描述
*The optimal dilutions should be determined by the end user.
*Tips:
WB: 适用于变性蛋白样本的免疫印迹检测. IHC: 适用于组织样本的石蜡(IHC-p)或冰冻(IHC-f)切片样本的免疫组化/荧光检测. IF/ICC: 适用于细胞样本的荧光检测. ELISA(peptide): 适用于抗原肽的ELISA检测.
引用格式: Affinity Biosciences Cat# DF9499, RRID:AB_2842695.
展开/折叠
DNA glycosylase/AP lyase Neil2; DNA-(apurinic or apyrimidinic site) lyase Neil2; Endonuclease 8-like 2; Endonuclease VIII-like 2; NEH2; Nei endonuclease VIII like 2 (E. coli); Nei endonuclease VIII like 2; Nei homolog 2; Nei-like 2; Nei-like protein 2; NEI2; NEIL2; NEIL2_HUMAN;
抗原和靶标
Detected in testis, skeletal muscle, heart, brain, placenta, lung, pancreas, kidney and liver.
- Q969S2 NEIL2_HUMAN:
- Protein BLAST With
- NCBI/
- ExPASy/
- Uniprot
MPEGPLVRKFHHLVSPFVGQQVVKTGGSSKKLQPASLQSLWLQDTQVHGKKLFLRFDLDEEMGPPGSSPTPEPPQKEVQKEGAADPKQVGEPSGQKTLDGSSRSAELVPQGEDDSEYLERDAPAGDAGRWLRVSFGLFGSVWVNDFSRAKKANKRGDWRDPSPRLVLHFGGGGFLAFYNCQLSWSSSPVVTPTCDILSEKFHRGQALEALGQAQPVCYTLLDQRYFSGLGNIIKNEALYRAGIHPLSLGSVLSASRREVLVDHVVEFSTAWLQGKFQGRPQHTQVYQKEQCPAGHQVMKEAFGPEDGLQRLTWWCPQCQPQLSEEPEQCQFS
种属预测
score>80的预测可信度较高，可尝试用于WB检测。*预测模型主要基于免疫原序列比对，结果仅作参考，不作为质保凭据。
High(score>80) Medium(80>score>50) Low(score<50) No confidence
翻译修饰 - Q969S2 作为底物
| Site | PTM Type | Enzyme | Source |
|---|---|---|---|
| K50 | Acetylation | Uniprot | |
| S68 | Phosphorylation | Uniprot | |
| T70 | Phosphorylation | Uniprot | |
| K96 | Ubiquitination | Uniprot | |
| K154 | Acetylation | Uniprot | |
| K234 | Ubiquitination | Uniprot | |
| K288 | Ubiquitination | Uniprot | |
| K299 | Ubiquitination | Uniprot |
研究背景
Involved in base excision repair of DNA damaged by oxidation or by mutagenic agents. Has DNA glycosylase activity towards 5-hydroxyuracil and other oxidized derivatives of cytosine with a preference for mismatched double-stranded DNA (DNA bubbles). Has low or no DNA glycosylase activity towards thymine glycol, 2-hydroxyadenine, hypoxanthine and 8-oxoguanine. Has AP (apurinic/apyrimidinic) lyase activity and introduces nicks in the DNA strand. Cleaves the DNA backbone by beta-delta elimination to generate a single-strand break at the site of the removed base with both 3'- and 5'-phosphates.
Nucleus.
Detected in testis, skeletal muscle, heart, brain, placenta, lung, pancreas, kidney and liver.
Binds EP300.
The zinc-finger domain is important for DNA binding.
Belongs to the FPG family.
研究领域
· Genetic Information Processing > Replication and repair > Base excision repair.
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