产品描述
*The optimal dilutions should be determined by the end user.
*Tips:
WB: 适用于变性蛋白样本的免疫印迹检测. IHC: 适用于组织样本的石蜡(IHC-p)或冰冻(IHC-f)切片样本的免疫组化/荧光检测. IF/ICC: 适用于细胞样本的荧光检测. ELISA(peptide): 适用于抗原肽的ELISA检测.
引用格式: Affinity Biosciences Cat# DF9504, RRID:AB_2842700.
展开/折叠
alpha-1,2-mannosidase EDEM3; C1orf22; EDEM 3; edem3; EDEM3_HUMAN; ER degradation enhancer, mannosidase alpha like 3; ER degradation enhancing alpha mannosidase like 3; ER degradation enhancing alpha mannosidase like protein 3; ER degradation-enhancing alpha-mannosidase-like 3;
抗原和靶标
- Q9BZQ6 EDEM3_HUMAN:
- Protein BLAST With
- NCBI/
- ExPASy/
- Uniprot
MSEAGGRGCGSPVPQRARWRLVAATAAFCLVSATSVWTAGAEPMSREEKQKLGNQVLEMFDHAYGNYMEHAYPADELMPLTCRGRVRGQEPSRGDVDDALGKFSLTLIDSLDTLVVLNKTKEFEDAVRKVLRDVNLDNDVVVSVFETNIRVLGGLLGGHSLAIMLKEKGEYMQWYNDELLQMAKQLGYKLLPAFNTTSGLPYPRINLKFGIRKPEARTGTETDTCTACAGTLILEFAALSRFTGATIFEEYARKALDFLWEKRQRSSNLVGVTINIHTGDWVRKDSGVGAGIDSYYEYLLKAYVLLGDDSFLERFNTHYDAIMRYISQPPLLLDVHIHKPMLNARTWMDALLAFFPGLQVLKGDIRPAIETHEMLYQVIKKHNFLPEAFTTDFRVHWAQHPLRPEFAESTYFLYKATGDPYYLEVGKTLIENLNKYARVPCGFAAMKDVRTGSHEDRMDSFFLAEMFKYLYLLFADKEDIIFDIEDYIFTTEAHLLPLWLSTTNQSISKKNTTSEYTELDDSNFDWTCPNTQILFPNDPLYAQSIREPLKNVVDKSCPRGIIRVEESFRSGAKPPLRARDFMATNPEHLEILKKMGVSLIHLKDGRVQLVQHAIQAASSIDAEDGLRFMQEMIELSSQQQKEQQLPPRAVQIVSHPFFGRVVLTAGPAQFGLDLSKHKETRGFVASSKPSNGCSELTNPEAVMGKIALIQRGQCMFAEKARNIQNAGAIGGIVIDDNEGSSSDTAPLFQMAGDGKDTDDIKIPMLFLFSKEGSIILDAIREYEEVEVLLSDKAKDRDPEMENEEQPSSENDSQNQSGEQISSSSQEVDLVDQESSEENSLNSHPESLSLADMDNAASISPSEQTSNPTENHETTNLNGECTDLDNQLQEQSETEEDSNPNVSWGKKVQPIDSILADWNEDIEAFEMMEKDEL
种属预测
score>80的预测可信度较高，可尝试用于WB检测。*预测模型主要基于免疫原序列比对，结果仅作参考，不作为质保凭据。
High(score>80) Medium(80>score>50) Low(score<50) No confidence
翻译修饰 - Q9BZQ6 作为底物
| Site | PTM Type | Enzyme | Source |
|---|---|---|---|
| K189 | Ubiquitination | Uniprot | |
| N195 | N-Glycosylation | Uniprot | |
| K208 | Ubiquitination | Uniprot | |
| K262 | Ubiquitination | Uniprot | |
| S286 | Phosphorylation | Uniprot | |
| Y295 | Phosphorylation | Uniprot | |
| Y296 | Phosphorylation | Uniprot | |
| K427 | Ubiquitination | Uniprot | |
| K435 | Ubiquitination | Uniprot | |
| K447 | Ubiquitination | Uniprot | |
| K550 | Ubiquitination | Uniprot | |
| K555 | Ubiquitination | Uniprot | |
| K573 | Ubiquitination | Uniprot | |
| K594 | Ubiquitination | Uniprot | |
| S686 | Phosphorylation | Uniprot | |
| K705 | Ubiquitination | Uniprot | |
| K719 | Ubiquitination | Uniprot | |
| S835 | Phosphorylation | Uniprot |
研究背景
Involved in endoplasmic reticulum-associated degradation (ERAD). Accelerates the glycoprotein ERAD by proteasomes, by catalyzing mannose trimming from Man8GlcNAc2 to Man7GlcNAc2 in the N-glycans. Seems to have alpha 1,2-mannosidase activity (By similarity).
Endoplasmic reticulum lumen.
Contains a protease-associated domain (PA) of unknown function.
Belongs to the glycosyl hydrolase 47 family.
研究领域
· Genetic Information Processing > Folding, sorting and degradation > Protein processing in endoplasmic reticulum. (View pathway)
限制条款
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