产品描述
*The optimal dilutions should be determined by the end user.
*Tips:
WB: 适用于变性蛋白样本的免疫印迹检测. IHC: 适用于组织样本的石蜡(IHC-p)或冰冻(IHC-f)切片样本的免疫组化/荧光检测. IF/ICC: 适用于细胞样本的荧光检测. ELISA(peptide): 适用于抗原肽的ELISA检测.
引用格式: Affinity Biosciences Cat# DF9505, RRID:AB_2842701.
展开/折叠
12,3-ketoacyl-CoA reductase,short chain; 17 beta HSD 12; 17 beta hydroxysteroid dehydrogenase 12; 17-@beta-HSD XII; 17-@beta-hydroxysteroid dehydrogenase XII; 17-beta-HSD 12; 17-beta-hydroxysteroid dehydrogenase 12; 17beta HSD type 12; 3 ketoacyl CoA reductase; 3-ketoacyl-CoA reductase; DHB12_HUMAN; Estradiol 17 beta dehydrogenase 12; Estradiol 17-beta-dehydrogenase 12; HSD17B12; Hydroxysteroid (17-beta) dehydrogenase 12; KAR; KIK-I; Kik1; SDR12C1; Short chain dehydrogenase/reductase family 12C member 1; Steroid dehydrogenase homolog;
抗原和靶标
Expressed in most tissues tested. Highly expressed in the ovary and mammary. Expressed in platelets.
- Q53GQ0 DHB12_HUMAN:
- Protein BLAST With
- NCBI/
- ExPASy/
- Uniprot
MESALPAAGFLYWVGAGTVAYLALRISYSLFTALRVWGVGNEAGVGPGLGEWAVVTGSTDGIGKSYAEELAKHGMKVVLISRSKDKLDQVSSEIKEKFKVETRTIAVDFASEDIYDKIKTGLAGLEIGILVNNVGMSYEYPEYFLDVPDLDNVIKKMININILSVCKMTQLVLPGMVERSKGAILNISSGSGMLPVPLLTIYSATKTFVDFFSQCLHEEYRSKGVFVQSVLPYFVATKLAKIRKPTLDKPSPETFVKSAIKTVGLQSRTNGYLIHALMGSIISNLPSWIYLKIVMNMNKSTRAHYLKKTKKN
种属预测
score>80的预测可信度较高，可尝试用于WB检测。*预测模型主要基于免疫原序列比对，结果仅作参考，不作为质保凭据。
High(score>80) Medium(80>score>50) Low(score<50) No confidence
翻译修饰 - Q53GQ0 作为底物
| Site | PTM Type | Enzyme | Source |
|---|---|---|---|
| Y12 | Phosphorylation | Uniprot | |
| Y66 | Phosphorylation | Uniprot | |
| K72 | Acetylation | Uniprot | |
| K84 | Ubiquitination | Uniprot | |
| K86 | Ubiquitination | Uniprot | |
| S91 | Phosphorylation | Uniprot | |
| S92 | Phosphorylation | Uniprot | |
| K95 | Ubiquitination | Uniprot | |
| K97 | Ubiquitination | Uniprot | |
| S111 | Phosphorylation | Uniprot | |
| Y115 | Phosphorylation | Uniprot | |
| K117 | Acetylation | Uniprot | |
| K117 | Ubiquitination | Uniprot | |
| K119 | Ubiquitination | Uniprot | |
| S164 | Phosphorylation | Uniprot | |
| T169 | Phosphorylation | Uniprot | |
| K181 | Ubiquitination | Uniprot | |
| C215 | S-Nitrosylation | Uniprot | |
| K223 | Ubiquitination | Uniprot | |
| Y233 | Phosphorylation | Uniprot | |
| K238 | Ubiquitination | Uniprot | |
| K241 | Ubiquitination | Uniprot | |
| K244 | Ubiquitination | Uniprot | |
| S251 | Phosphorylation | Uniprot | |
| K257 | Ubiquitination | Uniprot | |
| K261 | Ubiquitination | Uniprot | |
| K299 | Ubiquitination | Uniprot |
研究背景
Catalyzes the second of the four reactions of the long-chain fatty acids elongation cycle. This endoplasmic reticulum-bound enzymatic process, allows the addition of two carbons to the chain of long- and very long-chain fatty acids/VLCFAs per cycle. This enzyme has a 3-ketoacyl-CoA reductase activity, reducing 3-ketoacyl-CoA to 3-hydroxyacyl-CoA, within each cycle of fatty acid elongation. Thereby, it may participate in the production of VLCFAs of different chain lengths that are involved in multiple biological processes as precursors of membrane lipids and lipid mediators. May also catalyze the transformation of estrone (E1) into estradiol (E2) and play a role in estrogen formation.
Endoplasmic reticulum membrane>Multi-pass membrane protein.
Expressed in most tissues tested. Highly expressed in the ovary and mammary. Expressed in platelets.
Interacts with ELOVL1 and LASS2.
The di-lysine motif confers endoplasmic reticulum localization for type I membrane proteins.
Belongs to the short-chain dehydrogenases/reductases (SDR) family. 17-beta-HSD 3 subfamily.
研究领域
· Metabolism > Lipid metabolism > Fatty acid elongation.
· Metabolism > Lipid metabolism > Steroid hormone biosynthesis.
· Metabolism > Lipid metabolism > Biosynthesis of unsaturated fatty acids.
· Metabolism > Global and overview maps > Metabolic pathways.
· Metabolism > Global and overview maps > Fatty acid metabolism.
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