产品描述
*The optimal dilutions should be determined by the end user.
*Tips:
WB: 适用于变性蛋白样本的免疫印迹检测. IHC: 适用于组织样本的石蜡(IHC-p)或冰冻(IHC-f)切片样本的免疫组化/荧光检测. IF/ICC: 适用于细胞样本的荧光检测. ELISA(peptide): 适用于抗原肽的ELISA检测.
引用格式: Affinity Biosciences Cat# AF0196, RRID:AB_2833389.
展开/折叠
CARD2; CK 8; CK-8; CK8; CYK8; CYKER; Cytokeratin endo A; Cytokeratin-8; DreK8; EndoA; K2C8; K2C8_HUMAN; K8; Keratin 8; Keratin type II cytoskeletal 8; Keratin, type II cytoskeletal 8; Keratin-8; KO; Krt 2.8; KRT8; MGC118110; MGC174782; MGC53564; MGC85764; sb:cb186; Type-II keratin Kb8;
抗原和靶标
Observed in muscle fibers accumulating in the costameres of myoplasm at the sarcolemma membrane in structures that contain dystrophin and spectrin. Expressed in gingival mucosa and hard palate of the oral cavity.
- P05787 K2C8_HUMAN:
- Protein BLAST With
- NCBI/
- ExPASy/
- Uniprot
MSIRVTQKSYKVSTSGPRAFSSRSYTSGPGSRISSSSFSRVGSSNFRGGLGGGYGGASGMGGITAVTVNQSLLSPLVLEVDPNIQAVRTQEKEQIKTLNNKFASFIDKVRFLEQQNKMLETKWSLLQQQKTARSNMDNMFESYINNLRRQLETLGQEKLKLEAELGNMQGLVEDFKNKYEDEINKRTEMENEFVLIKKDVDEAYMNKVELESRLEGLTDEINFLRQLYEEEIRELQSQISDTSVVLSMDNSRSLDMDSIIAEVKAQYEDIANRSRAEAESMYQIKYEELQSLAGKHGDDLRRTKTEISEMNRNISRLQAEIEGLKGQRASLEAAIADAEQRGELAIKDANAKLSELEAALQRAKQDMARQLREYQELMNVKLALDIEIATYRKLLEGEESRLESGMQNMSIHTKTTSGYAGGLSSAYGGLTSPGLSYSLGSSFGSGAGSSSFSRTSSSRAVVVKKIETRDGKLVSESSDVLPK
翻译修饰 - P05787 作为底物
Site | PTM Type | Enzyme | Source |
---|---|---|---|
S2 | Phosphorylation | Uniprot | |
T6 | Phosphorylation | Q96GD4 (AURKB) | Uniprot |
K8 | Acetylation | Uniprot | |
K8 | Methylation | Uniprot | |
K8 | Ubiquitination | Uniprot | |
S9 | Phosphorylation | P17612 (PRKACA) | Uniprot |
Y10 | Phosphorylation | Uniprot | |
K11 | Acetylation | Uniprot | |
K11 | Methylation | Uniprot | |
K11 | Sumoylation | Uniprot | |
K11 | Ubiquitination | Uniprot | |
S13 | O-Glycosylation | Uniprot | |
S13 | Phosphorylation | P17612 (PRKACA) | Uniprot |
T14 | Phosphorylation | Uniprot | |
S15 | O-Glycosylation | Uniprot | |
S15 | Phosphorylation | Uniprot | |
R18 | Methylation | Uniprot | |
S21 | Phosphorylation | Uniprot | |
S22 | Phosphorylation | Uniprot | |
R23 | Methylation | Uniprot | |
S24 | Phosphorylation | P17612 (PRKACA) | Uniprot |
Y25 | Phosphorylation | Uniprot | |
T26 | Phosphorylation | Uniprot | |
S27 | Phosphorylation | Uniprot | |
S31 | Phosphorylation | Uniprot | |
R32 | Methylation | Uniprot | |
S34 | Phosphorylation | P17612 (PRKACA) | Uniprot |
S35 | Phosphorylation | Uniprot | |
S36 | Phosphorylation | Uniprot | |
S37 | Phosphorylation | P17612 (PRKACA) | Uniprot |
S39 | Phosphorylation | Uniprot | |
R40 | Methylation | Uniprot | |
S43 | Phosphorylation | P17612 (PRKACA) | Uniprot |
S44 | Phosphorylation | Uniprot | |
R47 | Methylation | Uniprot | |
Y54 | Phosphorylation | Uniprot | |
S58 | Phosphorylation | Uniprot | |
T64 | Phosphorylation | Uniprot | |
T67 | Phosphorylation | Uniprot | |
S71 | Phosphorylation | Uniprot | |
S74 | Phosphorylation | P36507 (MAP2K2) , O15264 (MAPK13) , Q05655 (PRKCD) , P53778 (MAPK12) , Q16539 (MAPK14) , Q15759 (MAPK11) , Q02750 (MAP2K1) , P45983 (MAPK8) | Uniprot |
T89 | Phosphorylation | Uniprot | |
K92 | Ubiquitination | Uniprot | |
K96 | Ubiquitination | Uniprot | |
T97 | Phosphorylation | Uniprot | |
K101 | Acetylation | Uniprot | |
K101 | Methylation | Uniprot | |
K101 | Sumoylation | Uniprot | |
K101 | Ubiquitination | Uniprot | |
S104 | Phosphorylation | Uniprot | |
K108 | Acetylation | Uniprot | |
K108 | Ubiquitination | Uniprot | |
K117 | Acetylation | Uniprot | |
K117 | Ubiquitination | Uniprot | |
K122 | Acetylation | Uniprot | |
K122 | Methylation | Uniprot | |
K122 | Ubiquitination | Uniprot | |
S124 | Phosphorylation | Uniprot | |
K130 | Acetylation | Uniprot | |
K130 | Sumoylation | Uniprot | |
K130 | Ubiquitination | Uniprot | |
T131 | Phosphorylation | Uniprot | |
S134 | Phosphorylation | Uniprot | |
S142 | Phosphorylation | Uniprot | |
Y143 | Phosphorylation | Uniprot | |
K158 | Acetylation | Uniprot | |
K158 | Ubiquitination | Uniprot | |
K160 | Ubiquitination | Uniprot | |
K176 | Ubiquitination | Uniprot | |
K178 | Methylation | Uniprot | |
K178 | Ubiquitination | Uniprot | |
Y179 | Phosphorylation | Uniprot | |
K185 | Methylation | Uniprot | |
K185 | Ubiquitination | Uniprot | |
T187 | Phosphorylation | Uniprot | |
K197 | Sumoylation | Uniprot | |
K197 | Ubiquitination | Uniprot | |
K198 | Ubiquitination | Uniprot | |
Y204 | Phosphorylation | Uniprot | |
K207 | Acetylation | Uniprot | |
K207 | Ubiquitination | Uniprot | |
S212 | Phosphorylation | Uniprot | |
T218 | Phosphorylation | Uniprot | |
Y228 | Phosphorylation | Uniprot | |
S240 | Phosphorylation | Uniprot | |
T242 | Phosphorylation | Uniprot | |
S243 | Phosphorylation | Uniprot | |
S247 | Phosphorylation | Uniprot | |
S251 | Phosphorylation | Uniprot | |
S253 | Phosphorylation | Uniprot | |
S258 | Phosphorylation | Uniprot | |
K264 | Ubiquitination | Uniprot | |
Y267 | Phosphorylation | Uniprot | |
S274 | Phosphorylation | Uniprot | |
S280 | Phosphorylation | Uniprot | |
Y282 | Phosphorylation | Uniprot | |
K285 | Acetylation | Uniprot | |
K285 | Methylation | Uniprot | |
K285 | Sumoylation | Uniprot | |
K285 | Ubiquitination | Uniprot | |
Y286 | Phosphorylation | Uniprot | |
S291 | Phosphorylation | Uniprot | |
K295 | Acetylation | Uniprot | |
K295 | Ubiquitination | Uniprot | |
T303 | Phosphorylation | Uniprot | |
K304 | Sumoylation | Uniprot | |
K304 | Ubiquitination | Uniprot | |
T305 | Phosphorylation | Uniprot | |
S308 | Phosphorylation | Uniprot | |
S315 | Phosphorylation | Uniprot | |
K325 | Acetylation | Uniprot | |
K325 | Sumoylation | Uniprot | |
K325 | Ubiquitination | Uniprot | |
S330 | Phosphorylation | Uniprot | |
K347 | Acetylation | Uniprot | |
K347 | Ubiquitination | Uniprot | |
K352 | Acetylation | Uniprot | |
K352 | Ubiquitination | Uniprot | |
S354 | Phosphorylation | Uniprot | |
K364 | Sumoylation | Uniprot | |
Y374 | Phosphorylation | Uniprot | |
K393 | Acetylation | Uniprot | |
K393 | Sumoylation | Uniprot | |
K393 | Ubiquitination | Uniprot | |
S400 | Phosphorylation | Uniprot | |
S404 | Phosphorylation | Uniprot | |
S410 | Phosphorylation | Uniprot | |
T413 | Phosphorylation | Uniprot | |
T415 | Phosphorylation | Uniprot | |
T416 | Phosphorylation | Uniprot | |
S417 | Phosphorylation | P17612 (PRKACA) | Uniprot |
Y419 | Phosphorylation | Uniprot | |
S424 | Phosphorylation | Uniprot | |
S425 | Phosphorylation | Uniprot | |
Y427 | Phosphorylation | Uniprot | |
T431 | Phosphorylation | P28482 (MAPK1) | Uniprot |
S432 | Phosphorylation | P27361 (MAPK3) , P45983 (MAPK8) , P06493 (CDK1) | Uniprot |
S436 | Phosphorylation | Uniprot | |
Y437 | Phosphorylation | Uniprot | |
S438 | Phosphorylation | Uniprot | |
S441 | Phosphorylation | Uniprot | |
S442 | Phosphorylation | Uniprot | |
S445 | Phosphorylation | Uniprot | |
S449 | Phosphorylation | Uniprot | |
S450 | Phosphorylation | Uniprot | |
S451 | Phosphorylation | Uniprot | |
S453 | Phosphorylation | Uniprot | |
T455 | Phosphorylation | Uniprot | |
S456 | Phosphorylation | Uniprot | |
S457 | Phosphorylation | Uniprot | |
S458 | Phosphorylation | Uniprot | |
K464 | Acetylation | Uniprot | |
K465 | Acetylation | Uniprot | |
K465 | Ubiquitination | Uniprot | |
T468 | Phosphorylation | Uniprot | |
K472 | Acetylation | Uniprot | |
K472 | Sumoylation | Uniprot | |
K472 | Ubiquitination | Uniprot | |
S475 | O-Glycosylation | Uniprot | |
S475 | Phosphorylation | Uniprot | |
S477 | Phosphorylation | Uniprot | |
S478 | Phosphorylation | Uniprot | |
K483 | Acetylation | Uniprot |
研究背景
Together with KRT19, helps to link the contractile apparatus to dystrophin at the costameres of striated muscle.
Phosphorylation on serine residues is enhanced during EGF stimulation and mitosis. Ser-74 phosphorylation plays an important role in keratin filament reorganization.
O-glycosylated. O-GlcNAcylation at multiple sites increases solubility, and decreases stability by inducing proteasomal degradation.
O-glycosylated (O-GlcNAcylated), in a cell cycle-dependent manner.
Cytoplasm. Nucleus>Nucleoplasm. Nucleus matrix.
Observed in muscle fibers accumulating in the costameres of myoplasm at the sarcolemma membrane in structures that contain dystrophin and spectrin. Expressed in gingival mucosa and hard palate of the oral cavity.
Heterotetramer of two type I and two type II keratins. KRT8 associates with KRT18. Associates with KRT20. Interacts with PNN. When associated with KRT19, interacts with DMD. Interacts with TCHP. Interacts with APEX1. Interacts with GPER1. Interacts with EPPK1 (By similarity).
(Microbial infection) Interacts with hepatitis C virus/HCV core protein.
Belongs to the intermediate filament family.
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