产品描述
*The optimal dilutions should be determined by the end user.
*Tips:
WB: 适用于变性蛋白样本的免疫印迹检测. IHC: 适用于组织样本的石蜡(IHC-p)或冰冻(IHC-f)切片样本的免疫组化/荧光检测. IF/ICC: 适用于细胞样本的荧光检测. ELISA(peptide): 适用于抗原肽的ELISA检测.
引用格式: Affinity Biosciences Cat# DF9527, RRID:AB_2842723.
展开/折叠
1700022B05Rik; A disintegrin and metalloprotease domain 8; Adam8; Gamma ring complex protein 103 kDa; Gamma tubulin complex component 2; Gamma tubulin complex protein 2; Gamma-ring complex protein 103 kDa; Gamma-tubulin complex component 2; GCP 2; GCP-2; GCP2; GCP2_HUMAN; h103p; hGCP2; hGrip103; hSpc97; MGC138162; MGC83533; OTTHUMP00000020808; SPBC97; Spc97p; Spindle pole body protein Spc97 homolog; TUBGCP 2; Tubgcp2; Tubulin gamma complex associated protein 2;
抗原和靶标
- Q9BSJ2 GCP2_HUMAN:
- Protein BLAST With
- NCBI/
- ExPASy/
- Uniprot
MSEFRIHHDVNELLSLLRVHGGDGAEVYIDLLQKNRTPYVTTTVSAHSAKVKIAEFSRTPEDFLKKYDELKSKNTRNLDPLVYLLSKLTEDKETLQYLQQNAKERAELAAAAVGSSTTSINVPAAASKISMQELEELRKQLGSVATGSTLQQSLELKRKMLRDKQNKKNSGQHLPIFPAWVYERPALIGDFLIGAGISTDTALPIGTLPLASQESAVVEDLLYVLVGVDGRYVSAQPLAGRQSRTFLVDPNLDLSIRELVHRILPVAASYSAVTRFIEEKSSFEYGQVNHALAAAMRTLVKEHLILVSQLEQLHRQGLLSLQKLWFYIQPAMRTMDILASLATSVDKGECLGGSTLSLLHDRSFSYTGDSQAQELCLYLTKAASAPYFEVLEKWIYRGIIHDPYSEFMVEEHELRKERIQEDYNDKYWDQRYTIVQQQIPSFLQKMADKILSTGKYLNVVRECGHDVTCPVAKEIIYTLKERAYVEQIEKAFNYASKVLLDFLMEEKELVAHLRSIKRYFLMDQGDFFVHFMDLAEEELRKPVEDITPPRLEALLELALRMSTANTDPFKDDLKIDLMPHDLITQLLRVLAIETKQEKAMAHADPTELALSGLEAFSFDYIVKWPLSLIINRKALTRYQMLFRHMFYCKHVERQLCSVWISNKTAKQHSLHSAQWFAGAFTLRQRMLNFVQNIQYYMMFEVMEPTWHILEKNLKSASNIDDVLGHHTGFLDTCLKDCMLTNPELLKVFSKLMSVCVMFTNCMQKFTQSMKLDGELGGQTLEHSTVLGLPAGAEERARKELARKHLAEHADTVQLVSGFEATINKFDKNFSAHLLDLLARLSIYSTSDCEHGMASVISRLDFNGFYTERLERLSAERSQKATPQVPVLRGPPAPAPRVAVTAQ
种属预测
score>80的预测可信度较高，可尝试用于WB检测。*预测模型主要基于免疫原序列比对，结果仅作参考，不作为质保凭据。
High(score>80) Medium(80>score>50) Low(score<50) No confidence
翻译修饰 - Q9BSJ2 作为底物
| Site | PTM Type | Enzyme | Source |
|---|---|---|---|
| T37 | Phosphorylation | Uniprot | |
| Y39 | Phosphorylation | Uniprot | |
| K65 | Ubiquitination | Uniprot | |
| Y83 | Phosphorylation | Uniprot | |
| S86 | Phosphorylation | Uniprot | |
| K92 | Ubiquitination | Uniprot | |
| K103 | Ubiquitination | Uniprot | |
| K157 | Ubiquitination | Uniprot | |
| K426 | Ubiquitination | Uniprot | |
| K449 | Ubiquitination | Uniprot | |
| K455 | Ubiquitination | Uniprot | |
| T468 | Phosphorylation | Uniprot | |
| K480 | Ubiquitination | Uniprot | |
| K490 | Ubiquitination | Uniprot | |
| T584 | Phosphorylation | Uniprot | |
| T594 | Phosphorylation | Uniprot | |
| K595 | Ubiquitination | Uniprot | |
| K714 | Ubiquitination | Uniprot | |
| K735 | Ubiquitination | Uniprot | |
| K803 | Ubiquitination | Uniprot | |
| K827 | Acetylation | Uniprot | |
| S873 | Phosphorylation | Uniprot | |
| K879 | Ubiquitination | Uniprot | |
| T881 | Phosphorylation | Uniprot |
研究背景
Gamma-tubulin complex is necessary for microtubule nucleation at the centrosome.
Cytoplasm>Cytoskeleton>Microtubule organizing center>Centrosome.
Ubiquitously expressed.
Gamma-tubulin complex is composed of gamma-tubulin, TUBGCP2, TUBGCP3, TUBGCP4, TUBGCP5 and TUBGCP6. Interacts with ATF5; the ATF5:PCNT:polyglutamylated tubulin (PGT) tripartite unites the mother centriole and the pericentriolar material (PCM) in the centrosome.
Belongs to the TUBGCP family.
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