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G-CSF Antibody - Western blot analysis of CSF3 using Jurkat whole cell lysates.

G-CSF Antibody - DF9542 at 1/100 staining Human pancreatic cancer and adjacent nomal tissues by IHC-P.

G-CSF Antibody - DF9542 at 1/100 staining Rat kidney tissue by IHC-P.

$FIGURE 4 Transcriptome heterogeneity of different subsets of Kupffer cells. (A) t- SNE plots of the expression of representative marker genes for Kupffer cells. (B) UMAP plot of Kupffer cells by clusters (up) and group (down). CDL, control donor liver; FDL, fatty donor liver. (C) Violin plot showing the expression of marker genes in the indicated clusters of Kupffer cells. The colourful bars corresponding to specific clusters in (b). (D) Volcano plot shows differentially expressed genes between CDL (blue dots) and FDL Kupffer cells (red dots). The names of some significant genes are indicated in the plots. (E) The KEGG pathway enrichment analysis of differentially expressed genes (DEGs) among Kupffer cell subtypes. (F) Multiplexed immunofluorescence staining to validate the existence of CSF3 + Kupffer cells (KCs) in FDL sample, white arrows (CLEC4F + CSF3 + ). Scale bar, 20 μm. (G) Connection graph showing the intensity of interactions between one cell type to another in coloured circles. Interactions were evaluated among all cell types, including immune and non- immune fractions. The node size indicated the number of L- R interactions$

G-CSF Antibody - FIGURE 4 Transcriptome heterogeneity of different subsets of Kupffer cells.

产品:	G-CSF 抗体
货号:	DF9542
描述:	Rabbit polyclonal antibody to G-CSF
应用:	WB IHC
文献验证:
反应:	Human, Rat
预测:	Bovine, Sheep, Rabbit
分子量:	22 kDa; 22kD(Calculated).
蛋白号:	P09919
RRID:	AB_2842738

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阻断肽(封闭肽)是特异性结合目标抗体和阻断抗体结合的多肽。这些多肽包含抗体识别的抗原表位。与阻断肽结合的抗体不再与靶蛋白上的表位结合。例如，在免疫印迹(WB)和免疫组化(IHC)中，通过比较被阻断抗体和未阻断抗体的染色，可以看到哪种染色是特异性的。

规格	价格	库存
100ul	RMB¥ 2300	现货
200ul	RMB¥ 3000	现货

货期: 当天发货

联系销售

MSDS

说明书

COA

实验方案

WB Handbook

IHC Protocol

IF/ICC Protocol

产品描述

来源:

Rabbit

应用:

WB 1:1000-3000, IHC 1:50-1:200
*The optimal dilutions should be determined by the end user.
*Tips:

WB: 适用于变性蛋白样本的免疫印迹检测. IHC: 适用于组织样本的石蜡(IHC-p)或冰冻(IHC-f)切片样本的免疫组化/荧光检测. IF/ICC: 适用于细胞样本的荧光检测. ELISA(peptide): 适用于抗原肽的ELISA检测.

反应:

Human,Rat

预测:

Bovine(83%), Sheep(83%), Rabbit(82%)

克隆:

Polyclonal

特异性:

G-CSF Antibody detects endogenous levels of total G-CSF.

RRID:

AB_2842738
引用格式: Affinity Biosciences Cat# DF9542, RRID:AB_2842738.

偶联:

Unconjugated.

纯化:

The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).

保存:

Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.

别名:

展开/折叠

C17orf33; Colony stimulating factor 3 (granulocyte); Colony stimulating factor 3; CSF 3; CSF beta; CSF3; CSF3_HUMAN; CSF3OS; Csfg; Filgrastim; G-CSF; GCSA; GCSF; Granulocyte colony stimulating factor; Granulocyte colony-stimulating factor; Lenograstim; Macrophage granulocyte inducer 2; MGC45931; MGI 2; Pluripoietin;

抗原和靶标

免疫原:

Uniprot:

P09919(CSF3_HUMAN) >>Visit HPA database.

基因/基因ID:

CSF3(1440)

序列:

P09919 CSF3_HUMAN:
Protein BLAST With
NCBI/
ExPASy/
Uniprot

MAGPATQSPMKLMALQLLLWHSALWTVQEATPLGPASSLPQSFLLKCLEQVRKIQGDGAALQEKLVSECATYKLCHPEELVLLGHSLGIPWAPLSSCPSQALQLAGCLSQLHSGLFLYQGLLQALEGISPELGPTLDTLQLDVADFATTIWQQMEELGMAPALQPTQGAMPAFASAFQRRAGGVLVASHLQSFLEVSYRVLRHLAQP

种属预测

种属预测:

score>80的预测可信度较高，可尝试用于WB检测。*预测模型主要基于免疫原序列比对，结果仅作参考，不作为质保凭据。

Species

Results

Score

Bovine

Sheep

Rabbit

Dog

Pig

Horse

Xenopus

Zebrafish

Chicken

Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

翻译修饰 - P09919 作为底物

P09919

Site	PTM Type	Enzyme	Source
T6	Phosphorylation		Uniprot

研究背景

P09919_CSF3_HUMAN

功能:

Granulocyte/macrophage colony-stimulating factors are cytokines that act in hematopoiesis by controlling the production, differentiation, and function of 2 related white cell populations of the blood, the granulocytes and the monocytes-macrophages. This CSF induces granulocytes.

翻译修饰:

O-glycan consists of Gal-GalNAc disaccharide which can be modified with up to two sialic acid residues (done in recombinantly expressed G-CSF from CHO cells).

细胞定位:

Secreted.

亚基结构:

Monomer.

蛋白家族:

Belongs to the IL-6 superfamily.

研究领域

· Environmental Information Processing > Signaling molecules and interaction > Cytokine-cytokine receptor interaction. (View pathway)

· Environmental Information Processing > Signal transduction > PI3K-Akt signaling pathway. (View pathway)

· Environmental Information Processing > Signal transduction > Jak-STAT signaling pathway. (View pathway)

· Human Diseases > Infectious diseases: Parasitic > Malaria.

· Organismal Systems > Immune system > Hematopoietic cell lineage. (View pathway)

· Organismal Systems > Immune system > IL-17 signaling pathway. (View pathway)

文献引用

1). Ly6G+ Neutrophils and Interleukin-17 Are Essential in Protection against Rodent Malaria Caused by Plasmodium berghei ANKA. Research, 2024 [IF=11.0]

2). Single‐cell profiling reveals distinct immune phenotypes that contribute to ischaemia‐reperfusion injury after steatotic liver transplantation. Cell Proliferation, 2021 (PubMed: 34469018) [IF=5.9]

Application: IF/ICC Species: Rat Sample: Kupffer cells

FIGURE 4 Transcriptome heterogeneity of different subsets of Kupffer cells. (A) t- SNE plots of the expression of representative marker genes for Kupffer cells. (B) UMAP plot of Kupffer cells by clusters (up) and group (down). CDL, control donor liver; FDL, fatty donor liver. (C) Violin plot showing the expression of marker genes in the indicated clusters of Kupffer cells. The colourful bars corresponding to specific clusters in (b). (D) Volcano plot shows differentially expressed genes between CDL (blue dots) and FDL Kupffer cells (red dots). The names of some significant genes are indicated in the plots. (E) The KEGG pathway enrichment analysis of differentially expressed genes (DEGs) among Kupffer cell subtypes. (F) Multiplexed immunofluorescence staining to validate the existence of CSF3 + Kupffer cells (KCs) in FDL sample, white arrows (CLEC4F + CSF3 + ). Scale bar, 20 μm. (G) Connection graph showing the intensity of interactions between one cell type to another in coloured circles. Interactions were evaluated among all cell types, including immune and non- immune fractions. The node size indicated the number of L- R interactions

3). Plasmodium berghei TatD-like DNase hijacks host innate immunity by inhibiting the TLR9-NF-κB pathway. International Immunopharmacology, 2024 [IF=4.8]

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G-CSF Antibody - #DF9542