| 产品: | TRIM23 抗体 |
| 货号: | DF9551 |
| 描述: | Rabbit polyclonal antibody to TRIM23 |
| 应用: | WB IHC |
| 反应: | Human, Mouse, Rat |
| 预测: | Pig, Zebrafish, Bovine, Horse, Sheep, Rabbit, Dog, Chicken, Xenopus |
| 分子量: | 64 kDa; 64kD(Calculated). |
| 蛋白号: | P36406 |
| RRID: | AB_2842747 |
产品描述
*The optimal dilutions should be determined by the end user.
*Tips:
WB: 适用于变性蛋白样本的免疫印迹检测. IHC: 适用于组织样本的石蜡(IHC-p)或冰冻(IHC-f)切片样本的免疫组化/荧光检测. IF/ICC: 适用于细胞样本的荧光检测. ELISA(peptide): 适用于抗原肽的ELISA检测.
引用格式: Affinity Biosciences Cat# DF9551, RRID:AB_2842747.
展开/折叠
ADP ribosylation factor domain protein 1; ADP-ribosylation factor domain-containing protein 1; ARF domain protein 1; ARFD1; E3 ubiquitin-protein ligase TRIM23; GTP binding protein ARD 1; GTP-binding protein ARD-1; N-acetyltransferase ARD1 human homolog of; N-acetyltransferase homolog of S. cerevisiae ARD1; RING finger protein 46; TRI23_HUMAN; TRIM23; Tripartite motif protein TRIM23; Tripartite motif-containing protein 23;
抗原和靶标
- P36406 TRI23_HUMAN:
- Protein BLAST With
- NCBI/
- ExPASy/
- Uniprot
MATLVVNKLGAGVDSGRQGSRGTAVVKVLECGVCEDVFSLQGDKVPRLLLCGHTVCHDCLTRLPLHGRAIRCPFDRQVTDLGDSGVWGLKKNFALLELLERLQNGPIGQYGAAEESIGISGESIIRCDEDEAHLASVYCTVCATHLCSECSQVTHSTKTLAKHRRVPLADKPHEKTMCSQHQVHAIEFVCLEEGCQTSPLMCCVCKEYGKHQGHKHSVLEPEANQIRASILDMAHCIRTFTEEISDYSRKLVGIVQHIEGGEQIVEDGIGMAHTEHVPGTAENARSCIRAYFYDLHETLCRQEEMALSVVDAHVREKLIWLRQQQEDMTILLSEVSAACLHCEKTLQQDDCRVVLAKQEITRLLETLQKQQQQFTEVADHIQLDASIPVTFTKDNRVHIGPKMEIRVVTLGLDGAGKTTILFKLKQDEFMQPIPTIGFNVETVEYKNLKFTIWDVGGKHKLRPLWKHYYLNTQAVVFVVDSSHRDRISEAHSELAKLLTEKELRDALLLIFANKQDVAGALSVEEITELLSLHKLCCGRSWYIQGCDARSGMGLYEGLDWLSRQLVAAGVLDVA
种属预测
score>80的预测可信度较高，可尝试用于WB检测。*预测模型主要基于免疫原序列比对，结果仅作参考，不作为质保凭据。
High(score>80) Medium(80>score>50) Low(score<50) No confidence
翻译修饰 - P36406 作为底物
| Site | PTM Type | Enzyme | Source |
|---|---|---|---|
| K8 | Ubiquitination | Uniprot | |
| S15 | Phosphorylation | Uniprot | |
| S20 | Phosphorylation | Uniprot | |
| K171 | Ubiquitination | Uniprot | |
| K357 | Ubiquitination | Uniprot | |
| S386 | Phosphorylation | Uniprot | |
| K458 | Acetylation | Uniprot | |
| Y468 | Phosphorylation | Uniprot | |
| Y469 | Phosphorylation | Uniprot | |
| K501 | Ubiquitination | Uniprot | |
| S550 | Phosphorylation | Uniprot |
研究背景
Acts as an E3 ubiquitin-protein ligase. Plays an essential role in autophagy activation during viral infection. Mechanistically, activates TANK-binding kinase 1/TBK1 by facilitating its dimerization and ability to phosphorylate the selective autophagy receptor SQSTM1. In order to achieve this function, TRIM23 mediates 'Lys-27'-linked auto-ubiquitination of its ADP-ribosylation factor (ARF) domain to induce its GTPase activity and its recruitment to autophagosomes.
(Microbial infection) Mediates TRAF6 auto-ubiquitination in the presence of human cytomegalovirus protein UL144, resulting in the virally controlled activation of NF-kappa-B stimulation at early times of HCMV infection.
Cytoplasm. Endomembrane system. Golgi apparatus membrane. Lysosome membrane.
Note: Membrane-associated with the Golgi complex and lysosomal structures.
Homodimer. Interacts with PSCD1. Interacts with UBE2D2. Interacts with TBK1 (via N-terminal kinase domain) and p62/SQSTM1.
(Microbial infection) Interacts with human cytomegalovirus protein UL144; this interaction might cause autoubiquitination of TRAF6, leading to NF-kappa-B activation.
The RING-type zinc finger domain is responsible for E3 ubiquitin ligase activity. This domain is catalytically active as a dimer.
In the C-terminal section; belongs to the small GTPase superfamily. Arf family.
限制条款
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