产品: GNL3 抗体
货号: DF9566
描述: Rabbit polyclonal antibody to GNL3
应用: WB
反应: Human, Mouse, Rat
预测: Pig, Zebrafish, Bovine, Sheep, Rabbit, Dog
分子量: 62 kDa; 62kD(Calculated).
蛋白号: Q9BVP2
RRID: AB_2842762

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 100ul RMB¥ 2300 现货
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产品描述

来源:
Rabbit
应用:
WB 1:1000-3000
*The optimal dilutions should be determined by the end user.
*Tips:

WB: 适用于变性蛋白样本的免疫印迹检测. IHC: 适用于组织样本的石蜡(IHC-p)或冰冻(IHC-f)切片样本的免疫组化/荧光检测. IF/ICC: 适用于细胞样本的荧光检测. ELISA(peptide): 适用于抗原肽的ELISA检测.

反应:
Human,Mouse,Rat
预测:
Pig(89%), Zebrafish(82%), Bovine(100%), Sheep(100%), Rabbit(100%), Dog(100%)
克隆:
Polyclonal
特异性:
GNL3 Antibody detects endogenous levels of total GNL3.
RRID:
AB_2842762
引用格式: Affinity Biosciences Cat# DF9566, RRID:AB_2842762.
偶联:
Unconjugated.
纯化:
The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).
保存:
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
别名:

展开/折叠

C77032; E2 induced gene 3 protein; E2-induced gene 3 protein; E2IG3; Estradiol induced nucleotide binding protein; GNL3; GNL3_HUMAN; Guanine nucleotide binding protein like 3 (nucleolar); Guanine nucleotide binding protein like 3; Guanine nucleotide-binding protein-like 3; MGC800; NNP47; Novel nucleolar protein 47; NS; Nucleolar GTP binding protein 3; Nucleolar GTP-binding protein 3; Nucleostemin; Putative nucleotide binding protein estradiol induced;

抗原和靶标

免疫原:
Uniprot:
基因/基因ID:
表达:
Q9BVP2 GNL3_HUMAN:

Increased levels in lung tissue in cancer patients.

序列:
MKRPKLKKASKRMTCHKRYKIQKKVREHHRKLRKEAKKRGHKKPRKDPGVPNSAPFKEALLREAELRKQRLEELKQQQKLDRQKELEKKRKLETNPDIKPSNVEPMEKEFGLCKTENKAKSGKQNSKKLYCQELKKVIEASDVVLEVLDARDPLGCRCPQVEEAIVQSGQKKLVLILNKSDLVPKENLESWLNYLKKELPTVVFRASTKPKDKGKITKRVKAKKNAAPFRSEVCFGKEGLWKLLGGFQETCSKAIRVGVIGFPNVGKSSIINSLKQEQMCNVGVSMGLTRSMQVVPLDKQITIIDSPSFIVSPLNSSSALALRSPASIEVVKPMEAASAILSQADARQVVLKYTVPGYRNSLEFFTVLAQRRGMHQKGGIPNVEGAAKLLWSEWTGASLAYYCHPPTSWTPPPYFNESIVVDMKSGFNLEELEKNNAQSIRAIKGPHLANSILFQSSGLTNGIIEEKDIHEELPKRKERKQEEREDDKDSDQETVDEEVDENSSGMFAAEETGEALSEETTAGEQSTRSFILDKIIEEDDAYDFSTDYV

种属预测

种属预测:

score>80的预测可信度较高,可尝试用于WB检测。*预测模型主要基于免疫原序列比对,结果仅作参考,不作为质保凭据。

Species
Results
Score
Bovine
100
Sheep
100
Dog
100
Rabbit
100
Pig
89
Zebrafish
82
Chicken
78
Xenopus
64
Horse
0
Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

翻译修饰 - Q9BVP2 作为底物

Site PTM Type Enzyme
K5 Acetylation
K17 Acetylation
K46 Ubiquitination
S53 Phosphorylation
K57 Ubiquitination
K75 Ubiquitination
K79 Acetylation
K99 Sumoylation
K114 Sumoylation
K114 Ubiquitination
K135 Ubiquitination
K136 Ubiquitination
S141 Phosphorylation
K171 Ubiquitination
K179 Ubiquitination
K185 Ubiquitination
Y194 Phosphorylation
K196 Ubiquitination
K197 Ubiquitination
K224 Methylation
K224 Ubiquitination
R230 Methylation
K237 Acetylation
K237 Ubiquitination
K242 Ubiquitination
K253 Ubiquitination
K267 Ubiquitination
S273 Phosphorylation
K275 Sumoylation
K275 Ubiquitination
T289 Phosphorylation
S291 Phosphorylation
K299 Ubiquitination
S312 Phosphorylation
S324 Phosphorylation
K332 Ubiquitination
K352 Ubiquitination
T366 Phosphorylation
K377 Ubiquitination
K388 Sumoylation
K434 Ubiquitination
K444 Sumoylation
K444 Ubiquitination
S490 Phosphorylation
T494 Phosphorylation
S503 Phosphorylation
S517 Phosphorylation
S529 Phosphorylation
K534 Sumoylation
Y542 Phosphorylation
S545 Phosphorylation
Y548 Phosphorylation

研究背景

功能:

May be required to maintain the proliferative capacity of stem cells. Stabilizes MDM2 by preventing its ubiquitination, and hence proteasomal degradation (By similarity).

细胞定位:

Nucleus. Nucleus>Nucleolus.
Note: Shuttles between the nucleus and nucleolus.

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionSubcellular location
组织特异性:

Increased levels in lung tissue in cancer patients.

亚基结构:

Interacts with MDM2; this interaction stabilizes MDM2. Interaction with MDM2 occurs in the nucleoplasm and is triggered by a nucleolar release mechanism, such as mitosis-induced nucleolar disassembly (By similarity). Indirectly interacts with TP53, via MDM2-binding (By similarity).

蛋白家族:

The basic domain (B) allows nucleolar localization in the absence of GTP. The intermediate domain (I) inhibits nucleolar localization by the B domain and is required for exit from the nucleolus. Exit from the nucleolus to the nucleoplasm requires both the I and the acidic (A) domains, and may be triggered by GTP hydrolysis (By similarity).

In contrast to other GTP-binding proteins, this family is characterized by a circular permutation of the GTPase motifs described by a G4-G1-G3 pattern.

Belongs to the TRAFAC class YlqF/YawG GTPase family.

研究领域

· Genetic Information Processing > Translation > Ribosome biogenesis in eukaryotes.

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