产品: | GNL3 抗体 |
货号: | DF9566 |
描述: | Rabbit polyclonal antibody to GNL3 |
应用: | WB |
反应: | Human, Mouse, Rat |
预测: | Pig, Zebrafish, Bovine, Sheep, Rabbit, Dog |
分子量: | 62 kDa; 62kD(Calculated). |
蛋白号: | Q9BVP2 |
RRID: | AB_2842762 |
产品描述
*The optimal dilutions should be determined by the end user.
*Tips:
WB: 适用于变性蛋白样本的免疫印迹检测. IHC: 适用于组织样本的石蜡(IHC-p)或冰冻(IHC-f)切片样本的免疫组化/荧光检测. IF/ICC: 适用于细胞样本的荧光检测. ELISA(peptide): 适用于抗原肽的ELISA检测.
引用格式: Affinity Biosciences Cat# DF9566, RRID:AB_2842762.
展开/折叠
C77032; E2 induced gene 3 protein; E2-induced gene 3 protein; E2IG3; Estradiol induced nucleotide binding protein; GNL3; GNL3_HUMAN; Guanine nucleotide binding protein like 3 (nucleolar); Guanine nucleotide binding protein like 3; Guanine nucleotide-binding protein-like 3; MGC800; NNP47; Novel nucleolar protein 47; NS; Nucleolar GTP binding protein 3; Nucleolar GTP-binding protein 3; Nucleostemin; Putative nucleotide binding protein estradiol induced;
抗原和靶标
- Q9BVP2 GNL3_HUMAN:
- Protein BLAST With
- NCBI/
- ExPASy/
- Uniprot
MKRPKLKKASKRMTCHKRYKIQKKVREHHRKLRKEAKKRGHKKPRKDPGVPNSAPFKEALLREAELRKQRLEELKQQQKLDRQKELEKKRKLETNPDIKPSNVEPMEKEFGLCKTENKAKSGKQNSKKLYCQELKKVIEASDVVLEVLDARDPLGCRCPQVEEAIVQSGQKKLVLILNKSDLVPKENLESWLNYLKKELPTVVFRASTKPKDKGKITKRVKAKKNAAPFRSEVCFGKEGLWKLLGGFQETCSKAIRVGVIGFPNVGKSSIINSLKQEQMCNVGVSMGLTRSMQVVPLDKQITIIDSPSFIVSPLNSSSALALRSPASIEVVKPMEAASAILSQADARQVVLKYTVPGYRNSLEFFTVLAQRRGMHQKGGIPNVEGAAKLLWSEWTGASLAYYCHPPTSWTPPPYFNESIVVDMKSGFNLEELEKNNAQSIRAIKGPHLANSILFQSSGLTNGIIEEKDIHEELPKRKERKQEEREDDKDSDQETVDEEVDENSSGMFAAEETGEALSEETTAGEQSTRSFILDKIIEEDDAYDFSTDYV
种属预测
score>80的预测可信度较高,可尝试用于WB检测。*预测模型主要基于免疫原序列比对,结果仅作参考,不作为质保凭据。
High(score>80) Medium(80>score>50) Low(score<50) No confidence
翻译修饰 - Q9BVP2 作为底物
Site | PTM Type | Enzyme | Source |
---|---|---|---|
K5 | Acetylation | Uniprot | |
K17 | Acetylation | Uniprot | |
K46 | Ubiquitination | Uniprot | |
S53 | Phosphorylation | Uniprot | |
K57 | Ubiquitination | Uniprot | |
K75 | Ubiquitination | Uniprot | |
K79 | Acetylation | Uniprot | |
K99 | Sumoylation | Uniprot | |
K114 | Sumoylation | Uniprot | |
K114 | Ubiquitination | Uniprot | |
K135 | Ubiquitination | Uniprot | |
K136 | Ubiquitination | Uniprot | |
S141 | Phosphorylation | Uniprot | |
K171 | Ubiquitination | Uniprot | |
K179 | Ubiquitination | Uniprot | |
K185 | Ubiquitination | Uniprot | |
Y194 | Phosphorylation | Uniprot | |
K196 | Ubiquitination | Uniprot | |
K197 | Ubiquitination | Uniprot | |
K224 | Methylation | Uniprot | |
K224 | Ubiquitination | Uniprot | |
R230 | Methylation | Uniprot | |
K237 | Acetylation | Uniprot | |
K237 | Ubiquitination | Uniprot | |
K242 | Ubiquitination | Uniprot | |
K253 | Ubiquitination | Uniprot | |
K267 | Ubiquitination | Uniprot | |
S273 | Phosphorylation | Uniprot | |
K275 | Sumoylation | Uniprot | |
K275 | Ubiquitination | Uniprot | |
T289 | Phosphorylation | Uniprot | |
S291 | Phosphorylation | Uniprot | |
K299 | Ubiquitination | Uniprot | |
S312 | Phosphorylation | Uniprot | |
S324 | Phosphorylation | Uniprot | |
K332 | Ubiquitination | Uniprot | |
K352 | Ubiquitination | Uniprot | |
T366 | Phosphorylation | Uniprot | |
K377 | Ubiquitination | Uniprot | |
K388 | Sumoylation | Uniprot | |
K434 | Ubiquitination | Uniprot | |
K444 | Sumoylation | Uniprot | |
K444 | Ubiquitination | Uniprot | |
S490 | Phosphorylation | Uniprot | |
T494 | Phosphorylation | Uniprot | |
S503 | Phosphorylation | Uniprot | |
S517 | Phosphorylation | Uniprot | |
S529 | Phosphorylation | Uniprot | |
K534 | Sumoylation | Uniprot | |
Y542 | Phosphorylation | Uniprot | |
S545 | Phosphorylation | Uniprot | |
Y548 | Phosphorylation | Uniprot |
研究背景
May be required to maintain the proliferative capacity of stem cells. Stabilizes MDM2 by preventing its ubiquitination, and hence proteasomal degradation (By similarity).
Nucleus. Nucleus>Nucleolus.
Note: Shuttles between the nucleus and nucleolus.
Increased levels in lung tissue in cancer patients.
Interacts with MDM2; this interaction stabilizes MDM2. Interaction with MDM2 occurs in the nucleoplasm and is triggered by a nucleolar release mechanism, such as mitosis-induced nucleolar disassembly (By similarity). Indirectly interacts with TP53, via MDM2-binding (By similarity).
The basic domain (B) allows nucleolar localization in the absence of GTP. The intermediate domain (I) inhibits nucleolar localization by the B domain and is required for exit from the nucleolus. Exit from the nucleolus to the nucleoplasm requires both the I and the acidic (A) domains, and may be triggered by GTP hydrolysis (By similarity).
In contrast to other GTP-binding proteins, this family is characterized by a circular permutation of the GTPase motifs described by a G4-G1-G3 pattern.
Belongs to the TRAFAC class YlqF/YawG GTPase family.
研究领域
· Genetic Information Processing > Translation > Ribosome biogenesis in eukaryotes.
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