产品描述
来源:
Rabbit
应用:
WB 1:1000-3000, IHC 1:50-1:200
*The optimal dilutions should be determined by the end user.
*Tips:
*The optimal dilutions should be determined by the end user.
*Tips:
WB: 适用于变性蛋白样本的免疫印迹检测. IHC: 适用于组织样本的石蜡(IHC-p)或冰冻(IHC-f)切片样本的免疫组化/荧光检测. IF/ICC: 适用于细胞样本的荧光检测. ELISA(peptide): 适用于抗原肽的ELISA检测.
反应:
Human,Mouse,Rat
预测:
Pig(100%), Horse(100%), Sheep(90%), Rabbit(80%), Dog(90%)
克隆:
Polyclonal
特异性:
HEATR1 Antibody detects endogenous levels of total HEATR1.
RRID:
AB_2842765
引用格式: Affinity Biosciences Cat# DF9569, RRID:AB_2842765.
引用格式: Affinity Biosciences Cat# DF9569, RRID:AB_2842765.
偶联:
Unconjugated.
纯化:
The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).
保存:
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
别名:
展开/折叠
BAP28; HEAT repeat containing 1; HEAT repeat containing protein 1; Protein BAP28; RP11-385F5.3; UTP10; UTP10 small subunit (SSU) processome component homolog;
抗原和靶标
免疫原:
Uniprot:
基因/基因ID:
序列:
- Q9H583 HEAT1_HUMAN:
- Protein BLAST With
- NCBI/
- ExPASy/
- Uniprot
MTSLAQQLQRLALPQSDASLLSRDEVASLLFDPKEAATIDRDTAFAIGCTGLEELLGIDPSFEQFEAPLFSQLAKTLERSVQTKAVNKQLDENISLFLIHLSPYFLLKPAQKCLEWLIHRFHIHLYNQDSLIACVLPYHETRIFVRVIQLLKINNSKHRWFWLLPVKQSGVPLAKGTLITHCYKDLGFMDFICSLVTKSVKVFAEYPGSSAQLRVLLAFYASTIVSALVAAEDVSDNIIAKLFPYIQKGLKSSLPDYRAATYMIICQISVKVTMENTFVNSLASQIIKTLTKIPSLIKDGLSCLIVLLQRQKPESLGKKPFPHLCNVPDLITILHGISETYDVSPLLHYMLPHLVVSIIHHVTGEETEGMDGQIYKRHLEAILTKISLKNNLDHLLASLLFEEYISYSSQEEMDSNKVSLLNEQFLPLIRLLESKYPRTLDVVLEEHLKEIADLKKQELFHQFVSLSTSGGKYQFLADSDTSLMLSLNHPLAPVRILAMNHLKKIMKTSKEGVDESFIKEAVLARLGDDNIDVVLSAISAFEIFKEHFSSEVTISNLLNLFQRAELSKNGEWYEVLKIAADILIKEEILSENDQLSNQVVVCLLPFMVINNDDTESAEMKIAIYLSKSGICSLHPLLRGWEEALENVIKSTKPGKLIGVANQKMIELLADNINLGDPSSMLKMVEDLISVGEEESFNLKQKVTFHVILSVLVSCCSSLKETHFPFAIRVFSLLQKKIKKLESVITAVEIPSEWHIELMLDRGIPVELWAHYVEELNSTQRVAVEDSVFLVFSLKKFIYALKAPKSFPKGDIWWNPEQLKEDSRDYLHLLIGLFEMMLNGADAVHFRVLMKLFIKVHLEDVFQLFKFCSVLWTYGSSLSNPLNCSVKTVLQTQALYVGCAMLSSQKTQCKHQLASISSPVVTSLLINLGSPVKEVRRAAIQCLQALSGVASPFYLIIDHLISKAEEITSDAAYVIQDLATLFEELQREKKLKSHQKLSETLKNLLSCVYSCPSYIAKDLMKVLQGVNGEMVLSQLLPMAEQLLEKIQKEPTAVLKDEAMVLHLTLGKYNEFSVSLLNEDPKSLDIFIKAVHTTKELYAGMPTIQITALEKITKPFFAAISDEKVQQKLLRMLFDLLVNCKNSHCAQTVSSVFKGISVNAEQVRIELEPPDKAKPLGTVQQKRRQKMQQKKSQDLESVQEVGGSYWQRVTLILELLQHKKKLRSPQILVPTLFNLLSRCLEPLPQEQGNMEYTKQLILSCLLNICQKLSPDGGKIPKDILDEEKFNVELIVQCIRLSEMPQTHHHALLLLGTVAGIFPDKVLHNIMSIFTFMGANVMRLDDTYSFQVINKTVKMVIPALIQSDSGDSIEVSRNVEEIVVKIISVFVDALPHVPEHRRLPILVQLVDTLGAEKFLWILLILLFEQYVTKTVLAAAYGEKDAILEADTEFWFSVCCEFSVQHQIQSLMNILQYLLKLPEEKEETIPKAVSFNKSESQEEMLQVFNVETHTSKQLRHFKFLSVSFMSQLLSSNNFLKKVVESGGPEILKGLEERLLETVLGYISAVAQSMERNADKLTVKFWRALLSKAYDLLDKVNALLPTETFIPVIRGLVGNPLPSVRRKALDLLNNKLQQNISWKKTIVTRFLKLVPDLLAIVQRKKKEGEEEQAINRQTALYTLKLLCKNFGAENPDPFVPVLNTAVKLIAPERKEEKNVLGSALLCIAEVTSTLEALAIPQLPSLMPSLLTTMKNTSELVSSEVYLLSALAALQKVVETLPHFISPYLEGILSQVIHLEKITSEMGSASQANIRLTSLKKTLATTLAPRVLLPAIKKTYKQIEKNWKNHMGPFMSILQEHIGVMKKEELTSHQSQLTAFFLEALDFRAQHSENDLEEVGKTENCIIDCLVAMVVKLSEVTFRPLFFKLFDWAKTEDAPKDRLLTFYNLADCIAEKLKGLFTLFAGHLVKPFADTLNQVNISKTDEAFFDSENDPEKCCLLLQFILNCLYKIFLFDTQHFISKERAEALMMPLVDQLENRLGGEEKFQERVTKHLIPCIAQFSVAMADDSLWKPLNYQILLKTRDSSPKVRFAALITVLALAEKLKENYIVLLPESIPFLAELMEDECEEVEHQCQKTIQQLETVLGEPLQSYF
种属预测
种属预测:
score>80的预测可信度较高，可尝试用于WB检测。*预测模型主要基于免疫原序列比对，结果仅作参考，不作为质保凭据。
Species
Results
Score
Pig
100
Horse
100
Sheep
90
Dog
90
Rabbit
80
Bovine
0
Xenopus
0
Zebrafish
0
Chicken
0
Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence
High(score>80) Medium(80>score>50) Low(score<50) No confidence
翻译修饰 - Q9H583 作为底物
| Site | PTM Type | Enzyme | Source |
|---|---|---|---|
| M1 | Acetylation | Uniprot | |
| T2 | Acetylation | Uniprot | |
| T2 | Phosphorylation | Uniprot | |
| R10 | Methylation | Uniprot | |
| S16 | Phosphorylation | Uniprot | |
| S19 | Phosphorylation | Uniprot | |
| K34 | Ubiquitination | Uniprot | |
| K84 | Ubiquitination | Uniprot | |
| K175 | Ubiquitination | Uniprot | |
| K248 | Ubiquitination | Uniprot | |
| K292 | Ubiquitination | Uniprot | |
| T363 | Phosphorylation | Uniprot | |
| T367 | Phosphorylation | Uniprot | |
| Y375 | Phosphorylation | Uniprot | |
| K435 | Ubiquitination | Uniprot | |
| K503 | Ubiquitination | Uniprot | |
| K510 | Ubiquitination | Uniprot | |
| S516 | Phosphorylation | Uniprot | |
| K519 | Ubiquitination | Uniprot | |
| S539 | Phosphorylation | Uniprot | |
| K568 | Ubiquitination | Uniprot | |
| K627 | Ubiquitination | Uniprot | |
| K655 | Ubiquitination | Uniprot | |
| K663 | Ubiquitination | Uniprot | |
| S709 | Phosphorylation | Uniprot | |
| K801 | Ubiquitination | Uniprot | |
| K808 | Ubiquitination | Uniprot | |
| K909 | Ubiquitination | Uniprot | |
| S917 | Phosphorylation | Uniprot | |
| S929 | Phosphorylation | Uniprot | |
| K995 | Ubiquitination | Uniprot | |
| K1047 | Ubiquitination | Uniprot | |
| K1054 | Ubiquitination | Uniprot | |
| Y1067 | Phosphorylation | Uniprot | |
| K1093 | Ubiquitination | Uniprot | |
| T1105 | Phosphorylation | Uniprot | |
| K1112 | Ubiquitination | Uniprot | |
| S1119 | Phosphorylation | Uniprot | |
| K1122 | Ubiquitination | Uniprot | |
| K1152 | Ubiquitination | Uniprot | |
| K1170 | Ubiquitination | Uniprot | |
| K1172 | Ubiquitination | Uniprot | |
| K1180 | Ubiquitination | Uniprot | |
| K1189 | Ubiquitination | Uniprot | |
| S1190 | Phosphorylation | Uniprot | |
| S1195 | Phosphorylation | Uniprot | |
| K1272 | Ubiquitination | Uniprot | |
| K1275 | Ubiquitination | Uniprot | |
| T1340 | Phosphorylation | Uniprot | |
| Y1341 | Phosphorylation | Uniprot | |
| S1360 | Phosphorylation | Uniprot | |
| K1477 | Ubiquitination | Uniprot | |
| K1483 | Ubiquitination | Uniprot | |
| S1486 | Phosphorylation | Uniprot | |
| K1489 | Ubiquitination | Uniprot | |
| S1490 | Phosphorylation | Uniprot | |
| S1492 | Phosphorylation | Uniprot | |
| K1508 | Ubiquitination | Uniprot | |
| S1517 | Phosphorylation | Uniprot | |
| S1519 | Phosphorylation | Uniprot | |
| S1526 | Phosphorylation | Uniprot | |
| S1527 | Phosphorylation | Uniprot | |
| K1533 | Ubiquitination | Uniprot | |
| S1537 | Phosphorylation | Uniprot | |
| K1575 | Ubiquitination | Uniprot | |
| T1597 | Phosphorylation | Uniprot | |
| T1599 | Phosphorylation | Uniprot | |
| S1614 | Phosphorylation | Uniprot | |
| K1618 | Ubiquitination | Uniprot | |
| K1626 | Ubiquitination | Uniprot | |
| K1634 | Ubiquitination | Uniprot | |
| K1655 | Acetylation | Uniprot | |
| K1675 | Ubiquitination | Uniprot | |
| K1679 | Acetylation | Uniprot | |
| K1679 | Ubiquitination | Uniprot | |
| S1794 | Phosphorylation | Uniprot | |
| K1811 | Ubiquitination | Uniprot | |
| T1815 | Phosphorylation | Uniprot | |
| T1816 | Phosphorylation | Uniprot | |
| R1820 | Methylation | Uniprot | |
| K1831 | Acetylation | Uniprot | |
| K1831 | Ubiquitination | Uniprot | |
| K1835 | Ubiquitination | Uniprot | |
| S1882 | Phosphorylation | Uniprot | |
| K1924 | Ubiquitination | Uniprot | |
| S1981 | Phosphorylation | Uniprot | |
| T2007 | Phosphorylation | Uniprot | |
| S2012 | Phosphorylation | Uniprot | |
| K2036 | Ubiquitination | Uniprot | |
| S2076 | Phosphorylation | Uniprot | |
| T2087 | Phosphorylation | Uniprot |
研究背景
功能:
Ribosome biogenesis factor. Involved in nucleolar processing of pre-18S ribosomal RNA. Required for optimal pre-ribosomal RNA transcription by RNA polymerase I.
细胞定位:
Nucleus>Nucleolus.
亚基结构:
May be a component of the proposed t-UTP subcomplex of the ribosomal small subunit (SSU) processome containing at least UTP4, WDR43, HEATR1, UTP15, WDR75.
蛋白家族:
Belongs to the HEATR1/UTP10 family.
研究领域
· Genetic Information Processing > Translation > Ribosome biogenesis in eukaryotes.
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