产品描述
*The optimal dilutions should be determined by the end user.
*Tips:
WB: 适用于变性蛋白样本的免疫印迹检测. IHC: 适用于组织样本的石蜡(IHC-p)或冰冻(IHC-f)切片样本的免疫组化/荧光检测. IF/ICC: 适用于细胞样本的荧光检测. ELISA(peptide): 适用于抗原肽的ELISA检测.
引用格式: Affinity Biosciences Cat# DF9571, RRID:AB_2842767.
展开/折叠
A+U-rich element RNA binding factor; AA407431; AA959857; AU rich element RNA binding factor; AU-rich element RNA-binding factor; D5Ertd650e; D5Wsu145e; Heterogeneous nuclear ribonucleoprotein D like; Heterogeneous nuclear ribonucleoprotein D like protein; Heterogeneous nuclear ribonucleoprotein D-like; hnHNRP DL; HNRDL_HUMAN; HNRNP; hnRNP D-like; hnRNP DL; HNRNPDL; hnRPD like protein; JKT41 binding protein; JKT41-binding protein; JKTBP; JKTBP2; laAUF1; MGC125262; Protein laAUF1;
抗原和靶标
Expressed in heart, brain, placenta, lung, liver, skeletal muscle, kidney, pancreas, spleen, thymus, prostate, testis, ovary, small intestine, colon and leukocytes. Expressed in myeloid leukemia, gastric adenocarcinoma, cervical carcinoma, hepatoma, fibrosarcoma, colon adenocarcinoma, epidermoid carcinoma, osteosarcoma and urinary bladder carcinoma cells.
- O14979 HNRDL_HUMAN:
- Protein BLAST With
- NCBI/
- ExPASy/
- Uniprot
MEVPPRLSHVPPPLFPSAPATLASRSLSHWRPRPPRQLAPLLPSLAPSSARQGARRAQRHVTAQQPSRLAGGAAIKGGRRRRPDLFRRHFKSSSIQRSAAAAAATRTARQHPPADSSVTMEDMNEYSNIEEFAEGSKINASKNQQDDGKMFIGGLSWDTSKKDLTEYLSRFGEVVDCTIKTDPVTGRSRGFGFVLFKDAASVDKVLELKEHKLDGKLIDPKRAKALKGKEPPKKVFVGGLSPDTSEEQIKEYFGAFGEIENIELPMDTKTNERRGFCFITYTDEEPVKKLLESRYHQIGSGKCEIKVAQPKEVYRQQQQQQKGGRGAAAGGRGGTRGRGRGQGQNWNQGFNNYYDQGYGNYNSAYGGDQNYSGYGGYDYTGYNYGNYGYGQGYADYSGQQSTYGKASRGGGNHQNNYQPY
种属预测
score>80的预测可信度较高，可尝试用于WB检测。*预测模型主要基于免疫原序列比对，结果仅作参考，不作为质保凭据。
High(score>80) Medium(80>score>50) Low(score<50) No confidence
翻译修饰 - O14979 作为底物
| Site | PTM Type | Enzyme | Source |
|---|---|---|---|
| R6 | Methylation | Uniprot | |
| S24 | Phosphorylation | Uniprot | |
| R25 | Methylation | Uniprot | |
| R68 | Methylation | Uniprot | |
| K76 | Methylation | Uniprot | |
| K76 | Ubiquitination | Uniprot | |
| R81 | Methylation | Uniprot | |
| R82 | Methylation | Uniprot | |
| S94 | Phosphorylation | Uniprot | |
| S98 | Phosphorylation | Uniprot | |
| S117 | Phosphorylation | Uniprot | |
| T119 | Phosphorylation | Uniprot | |
| Y126 | Phosphorylation | Uniprot | |
| S127 | Phosphorylation | Uniprot | |
| S136 | Phosphorylation | Uniprot | |
| K137 | Acetylation | Uniprot | |
| K137 | Ubiquitination | Uniprot | |
| K142 | Ubiquitination | Uniprot | |
| K149 | Ubiquitination | Uniprot | |
| S156 | Phosphorylation | Uniprot | |
| S160 | Phosphorylation | Uniprot | |
| K161 | Acetylation | Uniprot | |
| K161 | Methylation | Uniprot | |
| K161 | Ubiquitination | Uniprot | |
| K162 | Ubiquitination | Uniprot | |
| T165 | Phosphorylation | Uniprot | |
| Y167 | Phosphorylation | Uniprot | |
| S169 | Phosphorylation | Uniprot | |
| C177 | S-Nitrosylation | Uniprot | |
| T178 | Phosphorylation | Uniprot | |
| K180 | Acetylation | Uniprot | |
| K180 | Ubiquitination | Uniprot | |
| R189 | Methylation | Uniprot | |
| K197 | Acetylation | Uniprot | |
| K197 | Ubiquitination | Uniprot | |
| K204 | Acetylation | Uniprot | |
| K204 | Ubiquitination | Uniprot | |
| K209 | Ubiquitination | Uniprot | |
| K216 | Acetylation | Uniprot | |
| K216 | Ubiquitination | Uniprot | |
| K234 | Ubiquitination | Uniprot | |
| S241 | Phosphorylation | Uniprot | |
| T244 | Phosphorylation | Uniprot | |
| K269 | Ubiquitination | Uniprot | |
| K288 | Ubiquitination | Uniprot | |
| Y295 | Phosphorylation | Uniprot | |
| S300 | Phosphorylation | Uniprot | |
| K302 | Acetylation | Uniprot | |
| K302 | Ubiquitination | Uniprot | |
| K306 | Ubiquitination | Uniprot | |
| K311 | Ubiquitination | Uniprot | |
| Y314 | Phosphorylation | Uniprot | |
| Y387 | Phosphorylation | Uniprot | |
| Y389 | Phosphorylation | Uniprot | |
| Y396 | Phosphorylation | Uniprot | |
| R408 | Methylation | Uniprot | |
| Y417 | Phosphorylation | Uniprot | |
| Y420 | Phosphorylation | Uniprot |
研究背景
Acts as a transcriptional regulator. Promotes transcription repression. Promotes transcription activation in differentiated myotubes (By similarity). Binds to double- and single-stranded DNA sequences. Binds to the transcription suppressor CATR sequence of the COX5B promoter (By similarity). Binds with high affinity to RNA molecules that contain AU-rich elements (AREs) found within the 3'-UTR of many proto-oncogenes and cytokine mRNAs. Binds both to nuclear and cytoplasmic poly(A) mRNAs. Binds to poly(G) and poly(A), but not to poly(U) or poly(C) RNA homopolymers. Binds to the 5'-ACUAGC-3' RNA consensus sequence.
Dimethylation of Arg-408 is probably of the asymmetric type.
Nucleus. Cytoplasm.
Note: Shuttles between the nucleus and the cytoplasm in a TNPO1-dependent manner.
Expressed in heart, brain, placenta, lung, liver, skeletal muscle, kidney, pancreas, spleen, thymus, prostate, testis, ovary, small intestine, colon and leukocytes. Expressed in myeloid leukemia, gastric adenocarcinoma, cervical carcinoma, hepatoma, fibrosarcoma, colon adenocarcinoma, epidermoid carcinoma, osteosarcoma and urinary bladder carcinoma cells.
Interacts with ZNF148 (By similarity). Interacts with TNPO1.
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