(1)
(11)
产品描述
来源:
Rabbit
应用:
WB 1:1000-3000, IHC 1:50-1:200
*The optimal dilutions should be determined by the end user.
*Tips:
*The optimal dilutions should be determined by the end user.
*Tips:
WB: 适用于变性蛋白样本的免疫印迹检测. IHC: 适用于组织样本的石蜡(IHC-p)或冰冻(IHC-f)切片样本的免疫组化/荧光检测. IF/ICC: 适用于细胞样本的荧光检测. ELISA(peptide): 适用于抗原肽的ELISA检测.
反应:
Human,Mouse,Rat
预测:
Pig(100%), Bovine(100%), Horse(91%), Sheep(100%), Rabbit(100%), Dog(100%), Chicken(100%), Xenopus(91%)
克隆:
Polyclonal
特异性:
USP54 Antibody detects endogenous levels of total USP54.
RRID:
AB_2842791
引用格式: Affinity Biosciences Cat# DF9595, RRID:AB_2842791.
引用格式: Affinity Biosciences Cat# DF9595, RRID:AB_2842791.
偶联:
Unconjugated.
纯化:
The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).
保存:
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
别名:
展开/折叠
USP54; bA137L10.3; bA137L10.4; C10orf29; FLJ37318; Inactive ubiquitin carboxyl-terminal hydrolase 54; nactive ubiquitin-specific peptidase 54;
抗原和靶标
免疫原:
Uniprot:
基因/基因ID:
表达:
序列:
- Q70EL1 UBP54_HUMAN:
- Protein BLAST With
- NCBI/
- ExPASy/
- Uniprot
MSWKRNYFSGGRGSVQGMFAPRSSTSIAPSKGLSNEPGQNSCFLNSALQVLWHLDIFRRSFRQLTTHKCMGDSCIFCALKGIFNQFQCSSEKVLPSDTLRSALAKTFQDEQRFQLGIMDDAAECFENLLMRIHFHIADETKEDICTAQHCISHQKFAMTLFEQCVCTSCGATSDPLPFIQMVHYISTTSLCNQAICMLERREKPSPSMFGELLQNASTMGDLRNCPSNCGERIRIRRVLMNAPQIITIGLVWDSDHSDLAEDVIHSLGTCLKLGDLFFRVTDDRAKQSELYLVGMICYYGKHYSTFFFQTKIRKWMYFDDAHVKEIGPKWKDVVTKCIKGHYQPLLLLYADPQGTPVSTQDLPPQAEFQSYSRTCYDSEDSGREPSISSDTRTDSSTESYPYKHSHHESVVSHFSSDSQGTVIYNVENDSMSQSSRDTGHLTDSECNQKHTSKKGSLIERKRSSGRVRRKGDEPQASGYHSEGETLKEKQAPRNASKPSSSTNRLRDFKETVSNMIHNRPSLASQTNVGSHCRGRGGDQPDKKPPRTLPLHSRDWEIESTSSESKSSSSSKYRPTWRPKRESLNIDSIFSKDKRKHCGYTQLSPFSEDSAKEFIPDEPSKPPSYDIKFGGPSPQYKRWGPARPGSHLLEQHPRLIQRMESGYESSERNSSSPVSLDAALPESSNVYRDPSAKRSAGLVPSWRHIPKSHSSSILEVDSTASMGGWTKSQPFSGEEISSKSELDELQEEVARRAQEQELRRKREKELEAAKGFNPHPSRFMDLDELQNQGRSDGFERSLQEAESVFEESLHLEQKGDCAAALALCNEAISKLRLALHGASCSTHSRALVDKKLQISIRKARSLQDRMQQQQSPQQPSQPSACLPTQAGTLSQPTSEQPIPLQVLLSQEAQLESGMDTEFGASSFFHSPASCHESHSSLSPESSAPQHSSPSRSALKLLTSVEVDNIEPSAFHRQGLPKAPGWTEKNSHHSWEPLDAPEGKLQGSRCDNSSCSKLPPQEGRGIAQEQLFQEKKDPANPSPVMPGIATSERGDEHSLGCSPSNSSAQPSLPLYRTCHPIMPVASSFVLHCPDPVQKTNQCLQGQSLKTSLTLKVDRGSEETYRPEFPSTKGLVRSLAEQFQRMQGVSMRDSTGFKDRSLSGSLRKNSSPSDSKPPFSQGQEKGHWPWAKQQSSLEGGDRPLSWEESTEHSSLALNSGLPNGETSSGGQPRLAEPDIYQEKLSQVRDVRSKDLGSSTDLGTSLPLDSWVNITRFCDSQLKHGAPRPGMKSSPHDSHTCVTYPERNHILLHPHWNQDTEQETSELESLYQASLQASQAGCSGWGQQDTAWHPLSQTGSADGMGRRLHSAHDPGLSKTSTAEMEHGLHEARTVRTSQATPCRGLSRECGEDEQYSAENLRRISRSLSGTVVSEREEAPVSSHSFDSSNVRKPLETGHRCSSSSSLPVIHDPSVFLLGPQLYLPQPQFLSPDVLMPTMAGEPNRLPGTSRSVQQFLAMCDRGETSQGAKYTGRTLNYQSLPHRSRTDNSWAPWSETNQHIGTRFLTTPGCNPQLTYTATLPERSKGLQVPHTQSWSDLFHSPSHPPIVHPVYPPSSSLHVPLRSAWNSDPVPGSRTPGPRRVDMPPDDDWRQSSYASHSGHRRTVGEGFLFVLSDAPRREQIRARVLQHSQW
种属预测
种属预测:
score>80的预测可信度较高，可尝试用于WB检测。*预测模型主要基于免疫原序列比对，结果仅作参考，不作为质保凭据。
Species
Results
Score
Pig
100
Bovine
100
Sheep
100
Dog
100
Chicken
100
Rabbit
100
Horse
91
Xenopus
91
Zebrafish
0
Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence
High(score>80) Medium(80>score>50) Low(score<50) No confidence
翻译修饰 - Q70EL1 作为底物
| Site | PTM Type | Enzyme | Source |
|---|---|---|---|
| Y7 | Phosphorylation | Uniprot | |
| R12 | Methylation | Uniprot | |
| S14 | Phosphorylation | Uniprot | |
| S23 | Phosphorylation | Uniprot | |
| S24 | Phosphorylation | Uniprot | |
| K92 | Ubiquitination | Uniprot | |
| K105 | Ubiquitination | Uniprot | |
| K203 | Ubiquitination | Uniprot | |
| K331 | Ubiquitination | Uniprot | |
| K336 | Ubiquitination | Uniprot | |
| T374 | Phosphorylation | Uniprot | |
| Y376 | Phosphorylation | Uniprot | |
| S378 | Phosphorylation | Uniprot | |
| S381 | Phosphorylation | Uniprot | |
| S388 | Phosphorylation | Uniprot | |
| S389 | Phosphorylation | Uniprot | |
| T442 | Phosphorylation | Uniprot | |
| T451 | Phosphorylation | Uniprot | |
| S452 | Phosphorylation | Uniprot | |
| S456 | Phosphorylation | Uniprot | |
| S481 | Phosphorylation | Uniprot | |
| R533 | Methylation | Uniprot | |
| R535 | Methylation | Uniprot | |
| K542 | Methylation | Uniprot | |
| Y572 | Phosphorylation | Uniprot | |
| T575 | Phosphorylation | Uniprot | |
| S582 | Phosphorylation | Uniprot | |
| K591 | Ubiquitination | Uniprot | |
| S603 | Phosphorylation | Uniprot | |
| K620 | Ubiquitination | Uniprot | |
| S632 | Phosphorylation | Uniprot | |
| Y635 | Phosphorylation | Uniprot | |
| R653 | Methylation | Uniprot | |
| S660 | Phosphorylation | Uniprot | |
| Y662 | Phosphorylation | Uniprot | |
| S671 | Phosphorylation | Uniprot | |
| S674 | Phosphorylation | Uniprot | |
| S682 | Phosphorylation | Uniprot | |
| S690 | Phosphorylation | Uniprot | |
| K738 | Ubiquitination | Uniprot | |
| K769 | Ubiquitination | Uniprot | |
| S854 | Phosphorylation | Uniprot | |
| S958 | Phosphorylation | Uniprot | |
| K983 | Ubiquitination | Uniprot | |
| S988 | Phosphorylation | Uniprot | |
| K998 | Ubiquitination | Uniprot | |
| K1029 | Ubiquitination | Uniprot | |
| S1036 | Phosphorylation | Uniprot | |
| S1056 | Phosphorylation | Uniprot | |
| Y1069 | Phosphorylation | Uniprot | |
| K1103 | Ubiquitination | Uniprot | |
| K1126 | Ubiquitination | Uniprot | |
| S1143 | Phosphorylation | Uniprot | |
| S1156 | Phosphorylation | Uniprot | |
| S1158 | Phosphorylation | Uniprot | |
| K1161 | Ubiquitination | Uniprot | |
| S1163 | Phosphorylation | Uniprot | |
| S1164 | Phosphorylation | Uniprot | |
| K1169 | Ubiquitination | Uniprot | |
| Y1233 | Phosphorylation | Uniprot | |
| K1236 | Ubiquitination | Uniprot | |
| K1246 | Ubiquitination | Uniprot | |
| K1275 | Ubiquitination | Uniprot | |
| S1352 | Phosphorylation | Uniprot | |
| S1362 | Phosphorylation | Uniprot | |
| S1408 | Phosphorylation | Uniprot | |
| S1420 | Phosphorylation | Uniprot | |
| S1436 | Phosphorylation | Uniprot | |
| K1444 | Ubiquitination | Uniprot | |
| K1521 | Ubiquitination | Uniprot | |
| Y1529 | Phosphorylation | Uniprot | |
| S1531 | Phosphorylation | Uniprot | |
| Y1568 | Phosphorylation | Uniprot |
研究背景
功能:
Has no peptidase activity.
组织特异性:
Weakly expressed in a few tissues.
蛋白家族:
Belongs to the peptidase C19 family.
文献引用
1). Centrosomal protein 120 promotes centrosome amplification and gastric cancer progression via USP54-mediated deubiquitination of PLK4. iScience, 2021
(PubMed: 36590171)
[IF=5.8]
Application: WB Species: Human Sample: SGC7901 cells
Figure 5 USP54 combines with PLK4 and mediates its deubiquitination (A) Microarray analysis of mRNAs extracted from SGC7901-vector and SGC7901-CEP120 cells. (B) Results of the ubiquitination level of PLK4 in HEK293T cells, which were co-transfected with Flag-PLK4, HA-Ub, and one of the three DUBs. (C) WB results of USP54 and PLK4 expression in GC tissues. p value derived from Pearson correlation analysis. (D) WB results of PLK4 and USP54 after USP54 overexpression or knockdown. (E) CoIP analysis of the interaction between PLK4 and USP54 in SGC7901 and BGC823 cells. (F) HEK 293T cells were co-transfected with His-USP54 and Flag-PLK4 full length or Flag-PLK4 (1-740), Flag-PLK4 (1-270), and Flag-PLK4 (270-890) constructs and harvested for WB and IP analysis. (G) HEK 293T cells were co-transfected with His-USP54 and Flag-PLK4 wildtype or Flag-PLK4 lysine residue mutants and harvested for IP analysis. (H) Ubiquitination level of PLK4 after USP54 overexpression or knockdown examined by ubiquitination assay. (I) WB results of PLK4 in SGC7901 cells transfected with Lenti-CEP120 and siUSP54 and BGC823 cells transfected with Lenti-shCEP120 and USP54 plasmid.
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