ACSL1 Antibody - #DF9605

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产品: ACSL1 抗体
货号: DF9605
描述: Rabbit polyclonal antibody to ACSL1
应用: WB IHC
反应: Human, Mouse, Rat
预测: Bovine, Horse, Sheep, Dog, Chicken, Xenopus
分子量: 78 kDa; 78kD(Calculated).
蛋白号: P33121
RRID: AB_2842801

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   规格 价格 库存
 50ul RMB¥ 1500 现货
 100ul RMB¥ 2300 现货
 200ul RMB¥ 3000 现货

货期: 当天发货

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MS Report
HPLC Report
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实验方案
WB Handbook
IHC Protocol
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产品描述

来源:
Rabbit
应用:
WB 1:1000-3000, IHC 1:50-1:200
*The optimal dilutions should be determined by the end user.
*Tips:

WB: 适用于变性蛋白样本的免疫印迹检测. IHC: 适用于组织样本的石蜡(IHC-p)或冰冻(IHC-f)切片样本的免疫组化/荧光检测. IF/ICC: 适用于细胞样本的荧光检测. ELISA(peptide): 适用于抗原肽的ELISA检测.

反应:
Human,Mouse,Rat
预测:
Bovine(91%), Horse(100%), Sheep(100%), Dog(92%), Chicken(100%), Xenopus(100%)
克隆:
Polyclonal
特异性:
ACSL1 Antibody detects endogenous levels of total ACSL1.
RRID:
AB_2842801
引用格式: Affinity Biosciences Cat# DF9605, RRID:AB_2842801.
偶联:
Unconjugated.
纯化:
The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).
保存:
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
别名:

展开/折叠

ACS 1; ACS1; ACSL 1; ACSL1; ACSL1_HUMAN; Acyl CoA synthetase 1; Acyl CoA synthetase long chain family member 1; Acyl-CoA synthetase 1; FACL 1; FACL 2; FACL1; FACL2; Fatty acid Coenzyme A ligase long chain 1; Fatty acid Coenzyme A ligase long chain 2; LACS 1; LACS 2; LACS; LACS1; LACS2; Lignoceroyl CoA synthase; Long chain acyl CoA synthetase 1; Long chain acyl CoA synthetase 2; Long chain fatty acid CoA ligase 1; Long chain fatty acid CoA ligase 2; Long chain fatty acid coenzyme A ligase 1; Long-chain acyl-CoA synthetase 1; Long-chain acyl-CoA synthetase 2; Long-chain fatty acid-CoA ligase 2; Long-chain-fatty-acid--CoA ligase 1; Palmitoyl CoA ligase 1; Palmitoyl CoA ligase 2; Palmitoyl-CoA ligase 1; Palmitoyl-CoA ligase 2; Paltimoyl CoA ligase 1;

抗原和靶标

免疫原:
Uniprot:

P33121(ACSL1_HUMAN) >>Visit HPA database.

基因/基因ID:
ACSL1(2180)
表达:
P33121 ACSL1_HUMAN:

Highly expressed in liver, heart, skeletal muscle, kidney and erythroid cells, and to a lesser extent in brain, lung, placenta and pancreas.

序列:
MQAHELFRYFRMPELVDFRQYVRTLPTNTLMGFGAFAALTTFWYATRPKPLKPPCDLSMQSVEVAGSGGARRSALLDSDEPLVYFYDDVTTLYEGFQRGIQVSNNGPCLGSRKPDQPYEWLSYKQVAELSECIGSALIQKGFKTAPDQFIGIFAQNRPEWVIIEQGCFAYSMVIVPLYDTLGNEAITYIVNKAELSLVFVDKPEKAKLLLEGVENKLIPGLKIIVVMDAYGSELVERGQRCGVEVTSMKAMEDLGRANRRKPKPPAPEDLAVICFTSGTTGNPKGAMVTHRNIVSDCSAFVKATENTVNPCPDDTLISFLPLAHMFERVVECVMLCHGAKIGFFQGDIRLLMDDLKVLQPTVFPVVPRLLNRMFDRIFGQANTTLKRWLLDFASKRKEAELRSGIIRNNSLWDRLIFHKVQSSLGGRVRLMVTGAAPVSATVLTFLRAALGCQFYEGYGQTECTAGCCLTMPGDWTAGHVGAPMPCNLIKLVDVEEMNYMAAEGEGEVCVKGPNVFQGYLKDPAKTAEALDKDGWLHTGDIGKWLPNGTLKIIDRKKHIFKLAQGEYIAPEKIENIYMRSEPVAQVFVHGESLQAFLIAIVVPDVETLCSWAQKRGFEGSFEELCRNKDVKKAILEDMVRLGKDSGLKPFEQVKGITLHPELFSIDNGLLTPTMKAKRPELRNYFRSQIDDLYSTIKV

种属预测

种属预测:

score>80的预测可信度较高，可尝试用于WB检测。*预测模型主要基于免疫原序列比对，结果仅作参考，不作为质保凭据。

Species
Results
Score
Horse
100
Sheep
100
Xenopus
100
Chicken
100
Dog
92
Bovine
91
Rabbit
44
Pig
0
Zebrafish
0
Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

翻译修饰 - P33121 作为底物

Site PTM Type Enzyme
S103 Phosphorylation
S122 Phosphorylation
Y188 Phosphorylation
K216 Ubiquitination
K222 Ubiquitination
S232 Phosphorylation
K356 Ubiquitination
K386 Acetylation
S394 Phosphorylation
K395 Acetylation
K395 Ubiquitination
S410 Phosphorylation
K419 Ubiquitination
S439 Phosphorylation
K532 Ubiquitination
Y567 Phosphorylation
Y577 Phosphorylation
S620 Phosphorylation
K643 Ubiquitination
S664 Phosphorylation
T671 Phosphorylation
Y693 Phosphorylation
T695 Phosphorylation

研究背景

功能:

Catalyzes the conversion of long-chain fatty acids to their active form acyl-CoAs for both synthesis of cellular lipids, and degradation via beta-oxidation. Preferentially uses palmitoleate, oleate and linoleate. Preferentially activates arachidonate than epoxyeicosatrienoic acids (EETs) or hydroxyeicosatrienoic acids (HETEs) (By similarity).

细胞定位:

Mitochondrion outer membrane>Single-pass type III membrane protein. Peroxisome membrane>Single-pass type III membrane protein. Microsome membrane>Single-pass type III membrane protein. Endoplasmic reticulum membrane>Single-pass type III membrane protein.

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionSubcellular location
组织特异性:

Highly expressed in liver, heart, skeletal muscle, kidney and erythroid cells, and to a lesser extent in brain, lung, placenta and pancreas.

蛋白家族:

Belongs to the ATP-dependent AMP-binding enzyme family.

研究领域

· Cellular Processes > Transport and catabolism > Peroxisome.   (View pathway)

· Cellular Processes > Cell growth and death > Ferroptosis.   (View pathway)

· Metabolism > Lipid metabolism > Fatty acid biosynthesis.

· Metabolism > Lipid metabolism > Fatty acid degradation.

· Metabolism > Global and overview maps > Metabolic pathways.

· Metabolism > Global and overview maps > Fatty acid metabolism.

· Organismal Systems > Endocrine system > PPAR signaling pathway.

· Organismal Systems > Endocrine system > Adipocytokine signaling pathway.

文献引用

1). Dioscin ameliorates murine ulcerative colitis by regulating macrophage polarization. Pharmacological research, 2021 (PubMed: 34343656) [IF=9.3]
2). Fine particulate matter potentiates Th17-cell pathogenicity in experimental autoimmune uveitis via ferroptosis. ECOTOXICOLOGY AND ENVIRONMENTAL SAFETY, 2024 [IF=6.2]
3). Equisetin inhibits adiposity through AMPK-dependent regulation of brown adipocyte differentiation. Heliyon, 2024 (PubMed: 38327434) [IF=4.0]
4). ACSL1-Mediated Fatty Acid β-Oxidation Enhances Metastasis and Proliferation in Endometrial Cancer. Frontiers in bioscience (Landmark edition), 2024 (PubMed: 38420815) [IF=3.1]
5). MiR-103-3p promotes hepatic steatosis to aggravate nonalcoholic fatty liver disease by targeting of ACOX1. MOLECULAR BIOLOGY REPORTS, 2022 (PubMed: 35606603) [IF=2.8]

Application: WB    Species: Mice    Sample: liver tissue

Fig. 3 Antagomir-103-3p alleviated the damage to mice with NAFLD. Mice with NAFLD were fed an HFD for 8 weeks, and Antagomir-NC or Antagomir-103-3p was used for tail vein injection once a week for 2 weeks. A MiR-103-3p expression in mouse liver tissues was examined by qRT-PCR. B Oil Red O staining detected lipid droplet accumulation in mouse liver tissues, and HE staining detected liver tissue lesions in mice. C The TG, ALT, AST and H2O2 contents in mouse serum were examined, while ROS generation and ATP content were examined in mouse tissues. D The protein and mRNA levels of ACOX1, FASN and ACSL1 were examined by western blotting and qRT-PCR, respectively. *P < 0.05 compared with the control group; #P < 0.05 compared with the NAFLD+Antagomir-NC group

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