MLL2 Antibody - #DF9646

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产品: MLL2 抗体
货号: DF9646
描述: Rabbit polyclonal antibody to MLL2
应用: WB IHC IF/ICC
反应: Human
预测: Pig, Bovine, Sheep, Rabbit, Dog
分子量: 593kDa; 593kD(Calculated).
蛋白号: O14686
RRID: AB_2842842

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   规格 价格 库存
 100ul RMB¥ 2300 现货
 200ul RMB¥ 3000 现货

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IHC Protocol
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产品描述

来源:
Rabbit
应用:
WB 1:1000-3000, IHC 1:50-200, IF/ICC 1:100-1:500
*The optimal dilutions should be determined by the end user.
*Tips:

WB: 适用于变性蛋白样本的免疫印迹检测. IHC: 适用于组织样本的石蜡(IHC-p)或冰冻(IHC-f)切片样本的免疫组化/荧光检测. IF/ICC: 适用于细胞样本的荧光检测. ELISA(peptide): 适用于抗原肽的ELISA检测.

反应:
Human
预测:
Pig(100%), Bovine(100%), Sheep(100%), Rabbit(83%), Dog(100%)
克隆:
Polyclonal
特异性:
MLL2 Antibody detects endogenous levels of total MLL2.
RRID:
AB_2842842
引用格式: Affinity Biosciences Cat# DF9646, RRID:AB_2842842.
偶联:
Unconjugated.
纯化:
The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).
保存:
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
别名:

展开/折叠

AAD10; ALL1 related gene; ALL1-related protein; ALR; CAGL114; Histone-lysine N-methyltransferase MLL2; KABUK1; Kabuki make up syndrome; Kabuki mental retardation syndrome; KMS; KMT2B; KMT2D; Lysine N methyltransferase 2D; Lysine N-methyltransferase 2B; MLL2; MLL2_HUMAN; MLL4; Myeloid/lymphoid or mixed lineage leukemia 2; Myeloid/lymphoid or mixed-lineage leukemia protein 2; TNRC21; Trinucleotide repeat containing 21;

抗原和靶标

免疫原:
Uniprot:

O14686(KMT2D_HUMAN) >>Visit HPA database.

基因/基因ID:
KMT2D(8085)
表达:
O14686 KMT2D_HUMAN:

Expressed in most adult tissues, including a variety of hematoipoietic cells, with the exception of the liver.

序列:
MDSQKLAGEDKDSEPAADGPAASEDPSATESDLPNPHVGEVSVLSSGSPRLQETPQDCSGGPVRRCALCNCGEPSLHGQRELRRFELPFDWPRCPVVSPGGSPGPNEAVLPSEDLSQIGFPEGLTPAHLGEPGGSCWAHHWCAAWSAGVWGQEGPELCGVDKAIFSGISQRCSHCTRLGASIPCRSPGCPRLYHFPCATASGSFLSMKTLQLLCPEHSEGAAYLEEARCAVCEGPGELCDLFFCTSCGHHYHGACLDTALTARKRAGWQCPECKVCQACRKPGNDSKMLVCETCDKGYHTFCLKPPMEELPAHSWKCKACRVCRACGAGSAELNPNSEWFENYSLCHRCHKAQGGQTIRSVAEQHTPVCSRFSPPEPGDTPTDEPDALYVACQGQPKGGHVTSMQPKEPGPLQCEAKPLGKAGVQLEPQLEAPLNEEMPLLPPPEESPLSPPPEESPTSPPPEASRLSPPPEELPASPLPEALHLSRPLEESPLSPPPEESPLSPPPESSPFSPLEESPLSPPEESPPSPALETPLSPPPEASPLSPPFEESPLSPPPEELPTSPPPEASRLSPPPEESPMSPPPEESPMSPPPEASRLFPPFEESPLSPPPEESPLSPPPEASRLSPPPEDSPMSPPPEESPMSPPPEVSRLSPLPVVSRLSPPPEESPLSPPPEESPTSPPPEASRLSPPPEDSPTSPPPEDSPASPPPEDSLMSLPLEESPLLPLPEEPQLCPRSEGPHLSPRPEEPHLSPRPEEPHLSPQAEEPHLSPQPEEPCLCAVPEEPHLSPQAEGPHLSPQPEELHLSPQTEEPHLSPVPEEPCLSPQPEESHLSPQSEEPCLSPRPEESHLSPELEKPPLSPRPEKPPEEPGQCPAPEELPLFPPPGEPSLSPLLGEPALSEPGEPPLSPLPEELPLSPSGEPSLSPQLMPPDPLPPPLSPIITAAAPPALSPLGELEYPFGAKGDSDPESPLAAPILETPISPPPEANCTDPEPVPPMILPPSPGSPVGPASPILMEPLPPQCSPLLQHSLVPQNSPPSQCSPPALPLSVPSPLSPIGKVVGVSDEAELHEMETEKVSEPECPALEPSATSPLPSPMGDLSCPAPSPAPALDDFSGLGEDTAPLDGIDAPGSQPEPGQTPGSLASELKGSPVLLDPEELAPVTPMEVYPECKQTAGQGSPCEEQEEPRAPVAPTPPTLIKSDIVNEISNLSQGDASASFPGSEPLLGSPDPEGGGSLSMELGVSTDVSPARDEGSLRLCTDSLPETDDSLLCDAGTAISGGKAEGEKGRRRSSPARSRIKQGRSSSFPGRRRPRGGAHGGRGRGRARLKSTASSIETLVVADIDSSPSKEEEEEDDDTMQNTVVLFSNTDKFVLMQDMCVVCGSFGRGAEGHLLACSQCSQCYHPYCVNSKITKVMLLKGWRCVECIVCEVCGQASDPSRLLLCDDCDISYHTYCLDPPLLTVPKGGWKCKWCVSCMQCGAASPGFHCEWQNSYTHCGPCASLVTCPICHAPYVEEDLLIQCRHCERWMHAGCESLFTEDDVEQAADEGFDCVSCQPYVVKPVAPVAPPELVPMKVKEPEPQYFRFEGVWLTETGMALLRNLTMSPLHKRRQRRGRLGLPGEAGLEGSEPSDALGPDDKKDGDLDTDELLKGEGGVEHMECEIKLEGPVSPDVEPGKEETEESKKRKRKPYRPGIGGFMVRQRKSHTRTKKGPAAQAEVLSGDGQPDEVIPADLPAEGAVEQSLAEGDEKKKQQRRGRKKSKLEDMFPAYLQEAFFGKELLDLSRKALFAVGVGRPSFGLGTPKAKGDGGSERKELPTSQKGDDGPDIADEESRGLEGKADTPGPEDGGVKASPVPSDPEKPGTPGEGMLSSDLDRISTEELPKMESKDLQQLFKDVLGSEREQHLGCGTPGLEGSRTPLQRPFLQGGLPLGNLPSSSPMDSYPGLCQSPFLDSRERGGFFSPEPGEPDSPWTGSGGTTPSTPTTPTTEGEGDGLSYNQRSLQRWEKDEELGQLSTISPVLYANINFPNLKQDYPDWSSRCKQIMKLWRKVPAADKAPYLQKAKDNRAAHRINKVQKQAESQINKQTKVGDIARKTDRPALHLRIPPQPGALGSPPPAAAPTIFIGSPTTPAGLSTSADGFLKPPAGSVPGPDSPGELFLKLPPQVPAQVPSQDPFGLAPAYPLEPRFPTAPPTYPPYPSPTGAPAQPPMLGASSRPGAGQPGEFHTTPPGTPRHQPSTPDPFLKPRCPSLDNLAVPESPGVGGGKASEPLLSPPPFGESRKALEVKKEELGASSPSYGPPNLGFVDSPSSGTHLGGLELKTPDVFKAPLTPRASQVEPQSPGLGLRPQEPPPAQALAPSPPSHPDIFRPGSYTDPYAQPPLTPRPQPPPPESCCALPPRSLPSDPFSRVPASPQSQSSSQSPLTPRPLSAEAFCPSPVTPRFQSPDPYSRPPSRPQSRDPFAPLHKPPRPQPPEVAFKAGSLAHTSLGAGGFPAALPAGPAGELHAKVPSGQPPNFVRSPGTGAFVGTPSPMRFTFPQAVGEPSLKPPVPQPGLPPPHGINSHFGPGPTLGKPQSTNYTVATGNFHPSGSPLGPSSGSTGESYGLSPLRPPSVLPPPAPDGSLPYLSHGASQRSGITSPVEKREDPGTGMGSSLATAELPGTQDPGMSGLSQTELEKQRQRQRLRELLIRQQIQRNTLRQEKETAAAAAGAVGPPGSWGAEPSSPAFEQLSRGQTPFAGTQDKSSLVGLPPSKLSGPILGPGSFPSDDRLSRPPPPATPSSMDVNSRQLVGGSQAFYQRAPYPGSLPLQQQQQQLWQQQQATAATSMRFAMSARFPSTPGPELGRQALGSPLAGISTRLPGPGEPVPGPAGPAQFIELRHNVQKGLGPGGTPFPGQGPPQRPRFYPVSEDPHRLAPEGLRGLAVSGLPPQKPSAPPAPELNNSLHPTPHTKGPTLPTGLELVNRPPSSTELGRPNPLALEAGKLPCEDPELDDDFDAHKALEDDEELAHLGLGVDVAKGDDELGTLENLETNDPHLDDLLNGDEFDLLAYTDPELDTGDKKDIFNEHLRLVESANEKAEREALLRGVEPGPLGPEERPPPAADASEPRLASVLPEVKPKVEEGGRHPSPCQFTIATPKVEPAPAANSLGLGLKPGQSMMGSRDTRMGTGPFSSSGHTAEKASFGATGGPPAHLLTPSPLSGPGGSSLLEKFELESGALTLPGGPAASGDELDKMESSLVASELPLLIEDLLEHEKKELQKKQQLSAQLQPAQQQQQQQQQHSLLSAPGPAQAMSLPHEGSSPSLAGSQQQLSLGLAGARQPGLPQPLMPTQPPAHALQQRLAPSMAMVSNQGHMLSGQHGGQAGLVPQQSSQPVLSQKPMGTMPPSMCMKPQQLAMQQQLANSFFPDTDLDKFAAEDIIDPIAKAKMVALKGIKKVMAQGSIGVAPGMNRQQVSLLAQRLSGGPSSDLQNHVAAGSGQERSAGDPSQPRPNPPTFAQGVINEADQRQYEEWLFHTQQLLQMQLKVLEEQIGVHRKSRKALCAKQRTAKKAGREFPEADAEKLKLVTEQQSKIQKQLDQVRKQQKEHTNLMAEYRNKQQQQQQQQQQQQQQHSAVLALSPSQSPRLLTKLPGQLLPGHGLQPPQGPPGGQAGGLRLTPGGMALPGQPGGPFLNTALAQQQQQQHSGGAGSLAGPSGGFFPGNLALRSLGPDSRLLQERQLQLQQQRMQLAQKLQQQQQQQQQQQHLLGQVAIQQQQQQGPGVQTNQALGPKPQGLMPPSSHQGLLVQQLSPQPPQGPQGMLGPAQVAVLQQQHPGALGPQGPHRQVLMTQSRVLSSPQLAQQGQGLMGHRLVTAQQQQQQQQHQQQGSMAGLSHLQQSLMSHSGQPKLSAQPMGSLQQLQQQQQLQQQQQLQQQQQQQLQQQQQLQQQQLQQQQQQQQLQQQQQQQLQQQQQQLQQQQQQQQQQFQQQQQQQQMGLLNQSRTLLSPQQQQQQQVALGPGMPAKPLQHFSSPGALGPTLLLTGKEQNTVDPAVSSEATEGPSTHQGGPLAIGTTPESMATEPGEVKPSLSGDSQLLLVQPQPQPQPSSLQLQPPLRLPGQQQQQVSLLHTAGGGSHGQLGSGSSSEASSVPHLLAQPSVSLGDQPGSMTQNLLGPQQPMLERPMQNNTGPQPPKPGPVLQSGQGLPGVGIMPTVGQLRAQLQGVLAKNPQLRHLSPQQQQQLQALLMQRQLQQSQAVRQTPPYQEPGTQTSPLQGLLGCQPQLGGFPGPQTGPLQELGAGPRPQGPPRLPAPPGALSTGPVLGPVHPTPPPSSPQEPKRPSQLPSPSSQLPTEAQLPPTHPGTPKPQGPTLEPPPGRVSPAAAQLADTLFSKGLGPWDPPDNLAETQKPEQSSLVPGHLDQVNGQVVPEASQLSIKQEPREEPCALGAQSVKREANGEPIGAPGTSNHLLLAGPRSEAGHLLLQKLLRAKNVQLSTGRGSEGLRAEINGHIDSKLAGLEQKLQGTPSNKEDAAARKPLTPKPKRVQKASDRLVSSRKKLRKEDGVRASEALLKQLKQELSLLPLTEPAITANFSLFAPFGSGCPVNGQSQLRGAFGSGALPTGPDYYSQLLTKNNLSNPPTPPSSLPPTPPPSVQQKMVNGVTPSEELGEHPKDAASARDSERALRDTSEVKSLDLLAALPTPPHNQTEDVRMESDEDSDSPDSIVPASSPESILGEEAPRFPHLGSGRWEQEDRALSPVIPLIPRASIPVFPDTKPYGALGLEVPGKLPVTTWEKGKGSEVSVMLTVSAAAAKNLNGVMVAVAELLSMKIPNSYEVLFPESPARAGTEPKKGEAEGPGGKEKGLEGKSPDTGPDWLKQFDAVLPGYTLKSQLDILSLLKQESPAPEPPTQHSYTYNVSNLDVRQLSAPPPEEPSPPPSPLAPSPASPPTEPLVELPTEPLAEPPVPSPLPLASSPESARPKPRARPPEEGEDSRPPRLKKWKGVRWKRLRLLLTIQKGSGRQEDEREVAEFMEQLGTALRPDKVPRDMRRCCFCHEEGDGATDGPARLLNLDLDLWVHLNCALWSTEVYETQGGALMNVEVALHRGLLTKCSLCQRTGATSSCNRMRCPNVYHFACAIRAKCMFFKDKTMLCPMHKIKGPCEQELSSFAVFRRVYIERDEVKQIASIIQRGERLHMFRVGGLVFHAIGQLLPHQMADFHSATALYPVGYEATRIYWSLRTNNRRCCYRCSIGENNGRPEFVIKVIEQGLEDLVFTDASPQAVWNRIIEPVAAMRKEADMLRLFPEYLKGEELFGLTVHAVLRIAESLPGVESCQNYLFRYGRHPLMELPLMINPTGCARSEPKILTHYKRPHTLNSTSMSKAYQSTFTGETNTPYSKQFVHSKSSQYRRLRTEWKNNVYLARSRIQGLGLYAAKDLEKHTMVIEYIGTIIRNEVANRREKIYEEQNRGIYMFRINNEHVIDATLTGGPARYINHSCAPNCVAEVVTFDKEDKIIIISSRRIPKGEELTYDYQFDFEDDQHKIPCHCGAWNCRKWMN

种属预测

种属预测:

score>80的预测可信度较高，可尝试用于WB检测。*预测模型主要基于免疫原序列比对，结果仅作参考，不作为质保凭据。

Species
Results
Score
Pig
100
Bovine
100
Sheep
100
Dog
100
Rabbit
83
Horse
0
Xenopus
0
Zebrafish
0
Chicken
0
Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

翻译修饰 - O14686 作为底物

Site PTM Type Enzyme
S27 Phosphorylation
T29 Phosphorylation
S31 Phosphorylation
S48 Phosphorylation
T54 Phosphorylation
S102 Phosphorylation
Y223 Phosphorylation
Y298 Phosphorylation
S314 Phosphorylation
T366 Phosphorylation
S373 Phosphorylation
T380 Phosphorylation
Y389 Phosphorylation
S468 Phosphorylation
S477 Phosphorylation
S609 Phosphorylation
S618 Phosphorylation
S654 Phosphorylation
S952 Phosphorylation
S1037 Phosphorylation
S1065 Phosphorylation
S1151 Phosphorylation
T1164 Phosphorylation
S1180 Phosphorylation
T1195 Phosphorylation
T1198 Phosphorylation
S1249 Phosphorylation
S1263 Phosphorylation
T1267 Phosphorylation
S1270 Phosphorylation
S1293 Phosphorylation
S1294 Phosphorylation
S1298 Phosphorylation
S1307 Phosphorylation
S1331 Phosphorylation
T1332 Phosphorylation
S1334 Phosphorylation
S1335 Phosphorylation
S1606 Phosphorylation
K1610 Ubiquitination
T1647 Phosphorylation
S1671 Phosphorylation
K1690 Acetylation
Y1692 Phosphorylation
S1722 Phosphorylation
S1762 Phosphorylation
S1798 Phosphorylation
T1803 Phosphorylation
K1815 Acetylation
T1819 Phosphorylation
S1820 Phosphorylation
K1822 Acetylation
S1834 Phosphorylation
T1843 Phosphorylation
S1854 Phosphorylation
S1858 Phosphorylation
T1865 Phosphorylation
S1872 Phosphorylation
K1896 Ubiquitination
T1911 Phosphorylation
S1950 Phosphorylation
T2097 Phosphorylation
S2128 Phosphorylation
T2131 Phosphorylation
S2155 Phosphorylation
S2201 Phosphorylation
T2228 Phosphorylation
T2229 Phosphorylation
T2233 Phosphorylation
S2239 Phosphorylation
T2240 Phosphorylation
K2246 Acetylation
S2251 Phosphorylation
S2260 Phosphorylation
S2274 Phosphorylation
S2295 Phosphorylation
S2296 Phosphorylation
S2298 Phosphorylation
Y2299 Phosphorylation
S2309 Phosphorylation
S2311 Phosphorylation
T2314 Phosphorylation
T2323 Phosphorylation
T2332 Phosphorylation
S2342 Phosphorylation
T2426 Phosphorylation
S2451 Phosphorylation
K2468 Acetylation
R2471 Methylation
S2512 Phosphorylation
S2636 Phosphorylation
S2640 Phosphorylation
S2725 Phosphorylation
S2726 Phosphorylation
S2733 Phosphorylation
T2742 Phosphorylation
K2755 Acetylation
R2774 Methylation
R2860 Methylation
R2881 Methylation
K2886 Methylation
R2903 Methylation
R2905 Methylation
R2915 Methylation
R2922 Methylation
T2949 Phosphorylation
S2969 Phosphorylation
K3079 Acetylation
K3119 Acetylation
K3121 Acetylation
S3130 Phosphorylation
T3138 Phosphorylation
K3155 Acetylation
T3179 Phosphorylation
K3182 Acetylation
T3197 Phosphorylation
S3199 Phosphorylation
S3202 Phosphorylation
S3229 Phosphorylation
K3433 Acetylation
S3443 Phosphorylation
S3463 Phosphorylation
S3620 Phosphorylation
S3624 Phosphorylation
R3626 Methylation
R3719 Methylation
R3727 Methylation
S3832 Phosphorylation
S3836 Phosphorylation
S3837 Phosphorylation
R3851 Methylation
S3986 Phosphorylation
K4004 Acetylation
S4011 Phosphorylation
R4212 Methylation
S4215 Phosphorylation
S4359 Phosphorylation
S4430 Phosphorylation
S4456 Phosphorylation
K4465 Acetylation
K4465 Ubiquitination
K4470 Ubiquitination
S4475 Phosphorylation
K4501 Ubiquitination
T4505 Phosphorylation
K4509 Acetylation
K4516 Acetylation
T4519 Phosphorylation
S4534 Phosphorylation
K4553 Ubiquitination
S4617 Phosphorylation
T4621 Phosphorylation
S4624 Phosphorylation
S4625 Phosphorylation
T4629 Phosphorylation
S4633 Phosphorylation
T4643 Phosphorylation
S4661 Phosphorylation
T4682 Phosphorylation
T4688 Phosphorylation
S4727 Phosphorylation
S4738 Phosphorylation
K4756 Acetylation
K4756 Sumoylation
K4776 Acetylation
S4780 Phosphorylation
S4783 Phosphorylation
T4787 Phosphorylation
S4789 Phosphorylation
K4794 Acetylation
S4822 Phosphorylation
S4849 Phosphorylation
T4852 Phosphorylation
K4870 Ubiquitination
S4871 Phosphorylation
S4883 Phosphorylation
S4955 Phosphorylation
R4966 Methylation
S4974 Phosphorylation
R4975 Methylation
R4978 Methylation
K5024 Methylation
K5276 Acetylation
T5297 Phosphorylation
S5341 Phosphorylation
T5347 Phosphorylation
Y5349 Phosphorylation
S5361 Phosphorylation
T5374 Phosphorylation P24941 (CDK2)
K5378 Ubiquitination
Y5412 Phosphorylation
S5498 Phosphorylation
S5499 Phosphorylation

研究背景

功能:

Histone methyltransferase. Methylates 'Lys-4' of histone H3 (H3K4me). H3K4me represents a specific tag for epigenetic transcriptional activation. Acts as a coactivator for estrogen receptor by being recruited by ESR1, thereby activating transcription.

细胞定位:

Nucleus.

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionSubcellular location
组织特异性:

Expressed in most adult tissues, including a variety of hematoipoietic cells, with the exception of the liver.

亚基结构:

Component of the MLL2/3 complex (also named ASCOM complex), at least composed of KMT2D/MLL2 or KMT2C/MLL3, ASH2L, RBBP5, WDR5, NCOA6, DPY30, KDM6A, PAXIP1/PTIP, PAGR1 and alpha- and beta-tubulin. Interacts with ESR1; interaction is direct. Interacts (via WIN motif) with WDR5.

蛋白家族:

LXXLL motifs 5 and 6 are essential for the association with ESR1 nuclear receptor.

Belongs to the class V-like SAM-binding methyltransferase superfamily. Histone-lysine methyltransferase family. TRX/MLL subfamily.

研究领域

· Metabolism > Amino acid metabolism > Lysine degradation.

文献引用

1). KMT2D Regulates the NCOA6/THRB Signal Axis through Epigenetic Modification to Promote the Migration and Invasion of Papillary Thyroid Cancer. Frontiers in Bioscience - Elite (PubMed: 36722273)

Application: WB    Species: Human    Sample: Nthy-ori3-1, TPC, IHH-4 and BCPAP cells

Fig. 1. The mRNA and protein expressions of KMT2D in Nthy-ori3-1, TPC, IHH-4 and BCPAP cells. (a) qRT-PCR analysis. (b) Western blotting analysis. A: Nthy-ori3-1 cell; B: TPC cell; C: IHH-4 cell; D: BCPAP cell.

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