SLC22A4 Antibody - #DF9724

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产品: SLC22A4 抗体
货号: DF9724
描述: Rabbit polyclonal antibody to SLC22A4
应用: WB
反应: Human, Mouse
分子量: 62 kDa; 62kD(Calculated).
蛋白号: Q9H015
RRID: AB_2842919

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   规格 价格 库存
 100ul RMB¥ 2300 现货
 200ul RMB¥ 3000 现货

货期: 当天发货

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产品描述

来源:
Rabbit
应用:
WB 1:1000-3000
*The optimal dilutions should be determined by the end user.
*Tips:

WB: 适用于变性蛋白样本的免疫印迹检测. IHC: 适用于组织样本的石蜡(IHC-p)或冰冻(IHC-f)切片样本的免疫组化/荧光检测. IF/ICC: 适用于细胞样本的荧光检测. ELISA(peptide): 适用于抗原肽的ELISA检测.

反应:
Human,Mouse
克隆:
Polyclonal
特异性:
SLC22A4 Antibody detects endogenous levels of total SLC22A4.
RRID:
AB_2842919
引用格式: Affinity Biosciences Cat# DF9724, RRID:AB_2842919.
偶联:
Unconjugated.
纯化:
The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).
保存:
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
别名:

展开/折叠

Ergothioneine transporter; ET transporter; integral membrane transport protein; LSTP Like 2; MGC34546; MGC40524; Octn1; Organic cation transporter 1; Organic cation/carnitine transporter 1; RP23-354D12.1; S22A4_HUMAN; Slc22a4; Solute carrier family (organic cation transporter), member 4; solute carrier family 22 (organic cation/zwitterion transporter), member 4; Solute carrier family 22 member 4;

抗原和靶标

免疫原:
Uniprot:

Q9H015(S22A4_HUMAN) >>Visit HPA database.

基因/基因ID:
SLC22A4(6583)
表达:
Q9H015 S22A4_HUMAN:

Widely expressed. Highly expressed in whole blood, bone marrow, trachea and fetal liver. Weakly expressed in kidney, skeletal muscle, prostate, lung, pancreas, placenta, heart, uterus, spleen and spinal cord. Highly expressed in intestinal cell types affected by Crohn disease, including epithelial cells. Expressed in CD68 macrophage and CD43 T-cells but not in CD20 B-cells. Predominantly expressed in CD14 cells in peripheral blood mononuclear cells.

序列:
MRDYDEVIAFLGEWGPFQRLIFFLLSASIIPNGFNGMSVVFLAGTPEHRCRVPDAANLSSAWRNNSVPLRLRDGREVPHSCSRYRLATIANFSALGLEPGRDVDLGQLEQESCLDGWEFSQDVYLSTVVTEWNLVCEDNWKVPLTTSLFFVGVLLGSFVSGQLSDRFGRKNVLFATMAVQTGFSFLQIFSISWEMFTVLFVIVGMGQISNYVVAFILGTEILGKSVRIIFSTLGVCTFFAVGYMLLPLFAYFIRDWRMLLLALTVPGVLCVPLWWFIPESPRWLISQRRFREAEDIIQKAAKMNNIAVPAVIFDSVEELNPLKQQKAFILDLFRTRNIAIMTIMSLLLWMLTSVGYFALSLDAPNLHGDAYLNCFLSALIEIPAYITAWLLLRTLPRRYIIAAVLFWGGGVLLFIQLVPVDYYFLSIGLVMLGKFGITSAFSMLYVFTAELYPTLVRNMAVGVTSTASRVGSIIAPYFVYLGAYNRMLPYIVMGSLTVLIGILTLFFPESLGMTLPETLEQMQKVKWFRSGKKTRDSMETEENPKVLITAF

翻译修饰 - Q9H015 作为底物

Site PTM Type Enzyme
T88 Phosphorylation
S93 Phosphorylation
S468 Phosphorylation

研究背景

功能:

Sodium-ion dependent, low affinity carnitine transporter. Probably transports one sodium ion with one molecule of carnitine. Also transports organic cations such as tetraethylammonium (TEA) without the involvement of sodium. Relative uptake activity ratio of carnitine to TEA is 1.78. A key substrate of this transporter seems to be ergothioneine (ET).

细胞定位:

Membrane>Multi-pass membrane protein.

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionSubcellular location
组织特异性:

Widely expressed. Highly expressed in whole blood, bone marrow, trachea and fetal liver. Weakly expressed in kidney, skeletal muscle, prostate, lung, pancreas, placenta, heart, uterus, spleen and spinal cord. Highly expressed in intestinal cell types affected by Crohn disease, including epithelial cells. Expressed in CD68 macrophage and CD43 T-cells but not in CD20 B-cells. Predominantly expressed in CD14 cells in peripheral blood mononuclear cells.

亚基结构:

Interacts with PDZK1.

蛋白家族:

Belongs to the major facilitator (TC 2.A.1) superfamily. Organic cation transporter (TC 2.A.1.19) family.

研究领域

· Human Diseases > Cancers: Overview > Choline metabolism in cancer.   (View pathway)

文献引用

1). Cigarette smoke extract combined with lipopolysaccharide reduces OCTN1/2 expression in human alveolar epithelial cells in vitro and rat lung in vivo under inflammatory conditions. International Immunopharmacology, 2020 (PubMed: 32707498) [IF=5.6]

Application: WB    Species: human    Sample: A549 cells

Fig. 6. |The protein expression of OCTN1/2 and inflammatory factors in A549 cells at different concentrations. (A) The expression of OCTN1/2, IL-6, TNF-α, and IL1β in A549 cells was determined by western blot.
2). LPS-induced inflammation delays the transportation of ASP+ due to down-regulation of OCTN1/2 in alveolar epithelial cells. JOURNAL OF DRUG TARGETING, 2020 (PubMed: 31591905) [IF=4.5]

Application: WB    Species: human    Sample: A549 cells

Fig.2.| LPS regulates the level of OCTN1/2 and inflammation in a non-dose dependent manner in A549 cells. After cells were induced by different concentrations of LPS (0, 2, 10, 50, and 250 μg/mL) for 24 h, the expression of OCTN1 (62 kDa), OCTN2 (63 kDa), IL6 (24 kDa), IL18 (26 kDa), IL1β (31 kDa),and TNF-α (26 kDa) was significantly regulated in human respiratory epithelial cells;β-actin (42 kDa) was used as a loading control. Bands were analysed using ImageJ 1.45s (National Institutes of Health, USA). NC indicates the normal control groups. Data are shown as the mean ± SD (n = 3).

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