PREX1 Antibody - #DF9740

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产品: PREX1 抗体
货号: DF9740
描述: Rabbit polyclonal antibody to PREX1
应用: WB IHC
反应: Human, Mouse, Rat
预测: Pig, Zebrafish, Bovine, Horse, Sheep, Rabbit, Dog, Chicken
分子量: 186 kDa; 186kD(Calculated).
蛋白号: Q8TCU6
RRID: AB_2842935

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   规格 价格 库存
 100ul RMB¥ 2300 现货
 200ul RMB¥ 3000 现货

货期: 当天发货

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MS Report
HPLC Report
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WB Handbook
IHC Protocol
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产品描述

来源:
Rabbit
应用:
WB 1:1000-3000, IHC 1:50-1:200
*The optimal dilutions should be determined by the end user.
*Tips:

WB: 适用于变性蛋白样本的免疫印迹检测. IHC: 适用于组织样本的石蜡(IHC-p)或冰冻(IHC-f)切片样本的免疫组化/荧光检测. IF/ICC: 适用于细胞样本的荧光检测. ELISA(peptide): 适用于抗原肽的ELISA检测.

反应:
Human,Mouse,Rat
预测:
Pig(100%), Zebrafish(80%), Bovine(100%), Horse(100%), Sheep(100%), Rabbit(100%), Dog(100%), Chicken(90%)
克隆:
Polyclonal
特异性:
PREX1 Antibody detects endogenous levels of total PREX1.
RRID:
AB_2842935
引用格式: Affinity Biosciences Cat# DF9740, RRID:AB_2842935.
偶联:
Unconjugated.
纯化:
The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).
保存:
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
别名:

展开/折叠

4; 5)-dependent Rac exchanger 1; KIAA1415; OTTHUMP00000031228; P REX1; P Rex1 protein like; P-Rex1; Phosphatidylinositol 3,4,5 trisphosphate dependent Rac exchange factor 1; Phosphatidylinositol 3,4,5 trisphosphate dependent Rac exchanger 1; Phosphatidylinositol 3,4,5 trisphosphate dependent Rac exchanger 1 protein; Phosphatidylinositol 3,4,5-trisphosphate-dependent Rac exchanger 1 protein; Prex1; PREX1_HUMAN; PtdIns(3; PtdIns(3,4,5) dependent Rac exchanger 1; SET domain containing 6; Setd6;

抗原和靶标

免疫原:
Uniprot:

Q8TCU6(PREX1_HUMAN) >>Visit HPA database.

基因/基因ID:
PREX1(57580)
表达:
Q8TCU6 PREX1_HUMAN:

Mainly expressed in peripheral blood leukocytes and brain. Expressed at intermediate level in spleen and lymph nodes, and weakly expressed in other tissues.

序列:
MEAPSGSEPGGDGAGDCAHPDPRAPGAAAPSSGPGPCAAARESERQLRLRLCVLNEILGTERDYVGTLRFLQSAFLHRIRQNVADSVEKGLTEENVKVLFSNIEDILEVHKDFLAALEYCLHPEPQSQHELGNVFLKFKDKFCVYEEYCSNHEKALRLLVELNKIPTVRAFLLSCMLLGGRKTTDIPLEGYLLSPIQRICKYPLLLKELAKRTPGKHPDHPAVQSALQAMKTVCSNINETKRQMEKLEALEQLQSHIEGWEGSNLTDICTQLLLQGTLLKISAGNIQERAFFLFDNLLVYCKRKSRVTGSKKSTKRTKSINGSLYIFRGRINTEVMEVENVEDGTADYHSNGYTVTNGWKIHNTAKNKWFVCMAKTAEEKQKWLDAIIREREQRESLKLGMERDAYVMIAEKGEKLYHMMMNKKVNLIKDRRRKLSTVPKCFLGNEFVAWLLEIGEISKTEEGVNLGQALLENGIIHHVSDKHQFKNEQVMYRFRYDDGTYKARSELEDIMSKGVRLYCRLHSLYTPVIKDRDYHLKTYKSVLPGSKLVDWLLAQGDCQTREEAVALGVGLCNNGFMHHVLEKSEFRDESQYFRFHADEEMEGTSSKNKQLRNDFKLVENILAKRLLILPQEEDYGFDIEEKNKAVVVKSVQRGSLAEVAGLQVGRKIYSINEDLVFLRPFSEVESILNQSFCSRRPLRLLVATKAKEIIKIPDQPDTLCFQIRGAAPPYVYAVGRGSEAMAAGLCAGQCILKVNGSNVMNDGAPEVLEHFQAFRSRREEALGLYQWIYHTHEDAQEARASQEASTEDPSGEQAQEEDQADSAFPLLSLGPRLSLCEDSPMVTLTVDNVHLEHGVVYEYVSTAGVRCHVLEKIVEPRGCFGLTAKILEAFAANDSVFVENCRRLMALSSAIVTMPHFEFRNICDTKLESIGQRIACYQEFAAQLKSRVSPPFKQAPLEPHPLCGLDFCPTNCHINLMEVSYPKTTPSVGRSFSIRFGRKPSLIGLDPEQGHLNPMSYTQHCITTMAAPSWKCLPAAEGDPQGQGLHDGSFGPASGTLGQEDRGLSFLLKQEDREIQDAYLQLFTKLDVALKEMKQYVTQINRLLSTITEPTSGGSCDASLAEEASSLPLVSEESEMDRSDHGGIKKVCFKVAEEDQEDSGHDTMSYRDSYSECNSNRDSVLSYTSVRSNSSYLGSDEMGSGDELPCDMRIPSDKQDKLHGCLEHLFNQVDSINALLKGPVMSRAFEETKHFPMNHSLQEFKQKEECTIRGRSLIQISIQEDPWNLPNSIKTLVDNIQRYVEDGKNQLLLALLKCTDTELQLRRDAIFCQALVAAVCTFSKQLLAALGYRYNNNGEYEESSRDASRKWLEQVAATGVLLHCQSLLSPATVKEERTMLEDIWVTLSELDNVTFSFKQLDENYVANTNVFYHIEGSRQALKVIFYLDSYHFSKLPSRLEGGASLRLHTALFTKVLENVEGLPSPGSQAAEDLQQDINAQSLEKVQQYYRKLRAFYLERSNLPTDASTTAVKIDQLIRPINALDELCRLMKSFVHPKPGAAGSVGAGLIPISSELCYRLGACQMVMCGTGMQRSTLSVSLEQAAILARSHGLLPKCIMQATDIMRKQGPRVEILAKNLRVKDQMPQGAPRLYRLCQPPVDGDL

种属预测

种属预测:

score>80的预测可信度较高，可尝试用于WB检测。*预测模型主要基于免疫原序列比对，结果仅作参考，不作为质保凭据。

Species
Results
Score
Pig
100
Horse
100
Bovine
100
Sheep
100
Dog
100
Rabbit
100
Chicken
90
Zebrafish
80
Xenopus
70
Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

翻译修饰 - Q8TCU6 作为底物

Site PTM Type Enzyme
S174 Phosphorylation
K182 Ubiquitination
K201 Ubiquitination
K216 Ubiquitination
S235 Phosphorylation
K241 Ubiquitination
T308 Phosphorylation
S310 Phosphorylation
S313 Phosphorylation
S319 Phosphorylation
T333 Phosphorylation
S436 Phosphorylation P17612 (PRKACA)
T437 Phosphorylation
K482 Ubiquitination
T526 Phosphorylation
K537 Ubiquitination
S590 Phosphorylation
S605 Phosphorylation
K624 Ubiquitination
K642 Acetylation
K649 Ubiquitination
T704 Phosphorylation
S805 Phosphorylation
S834 Phosphorylation
K926 Ubiquitination
K945 Ubiquitination
S991 Phosphorylation
K999 Sumoylation
S1001 Phosphorylation
S1049 Phosphorylation
S1065 Phosphorylation
K1069 Ubiquitination
S1125 Phosphorylation
S1159 Phosphorylation
S1165 Phosphorylation
Y1166 Phosphorylation
S1169 Phosphorylation
S1179 Phosphorylation
S1182 Phosphorylation
S1191 Phosphorylation
S1195 Phosphorylation
S1200 Phosphorylation
R1209 Methylation
K1249 Ubiquitination
S1256 Phosphorylation
S1272 Phosphorylation
Y1442 Phosphorylation
K1500 Ubiquitination
T1525 Phosphorylation

研究背景

功能:

Functions as a RAC guanine nucleotide exchange factor (GEF), which activates the Rac proteins by exchanging bound GDP for free GTP. Its activity is synergistically activated by phosphatidylinositol 3,4,5-trisphosphate and the beta gamma subunits of heterotrimeric G protein. May function downstream of heterotrimeric G proteins in neutrophils.

细胞定位:

Cytoplasm>Cytosol. Cell membrane.
Note: Mainly cytosolic. Some amount is apparently associated to the plasma membrane.

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionSubcellular location
组织特异性:

Mainly expressed in peripheral blood leukocytes and brain. Expressed at intermediate level in spleen and lymph nodes, and weakly expressed in other tissues.

亚基结构:

Interacts preferentially with RAC2. Interacts with RAC1. Interacts with AUTS2.

研究领域

· Organismal Systems > Immune system > Chemokine signaling pathway.   (View pathway)

文献引用

1). PREX1 depletion ameliorates high-fat diet-induced non-alcoholic fatty liver disease in mice and mitigates palmitic acid-induced hepatocellular injury via suppressing the NF-κB signaling pathway. TOXICOLOGY AND APPLIED PHARMACOLOGY, 2022 (PubMed: 35605788) [IF=3.3]

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