产品描述
*The optimal dilutions should be determined by the end user.
*Tips:
WB: 适用于变性蛋白样本的免疫印迹检测. IHC: 适用于组织样本的石蜡(IHC-p)或冰冻(IHC-f)切片样本的免疫组化/荧光检测. IF/ICC: 适用于细胞样本的荧光检测. ELISA(peptide): 适用于抗原肽的ELISA检测.
引用格式: Affinity Biosciences Cat# DF9741, RRID:AB_2842936.
展开/折叠
5-bisphosphate 5-phosphatase A; Inositol polyphosphate 5-phosphatase; Inositol polyphosphate 5-phosphatase J; INPP5; Inpp5j; Phosphatidylinositol 4; Phosphatidylinositol 4,5-bisphosphate 5-phosphatase A; PI5PA_HUMAN; PIB5PA; PIPP;
抗原和靶标
- Q15735 PI5PA_HUMAN:
- Protein BLAST With
- NCBI/
- ExPASy/
- Uniprot
MEGQSSRGSRRPGTRAGLGSLPMPQGVAQTGAPSKVDSSFQLPAKKNAALGPSEPRLALAPVGPRAAMSASSEGPRLALASPRPILAPLCTPEGQKTATAHRSSSLAPTSVGQLVMSASAGPKPPPATTGSVLAPTSLGLVMPASAGPRSPPVTLGPNLAPTSRDQKQEPPASVGPKPTLAASGLSLALASEEQPPELPSTPSPVPSPVLSPTQEQALAPASTASGAASVGQTSARKRDAPAPRPLPASEGHLQPPAQTSGPTGSPPCIQTSPDPRLSPSFRARPEALHSSPEDPVLPRPPQTLPLDVGQGPSEPGTHSPGLLSPTFRPGAPSGQTVPPPLPKPPRSPSRSPSHSPNRSPCVPPAPDMALPRLGTQSTGPGRCLSPNLQAQEAPAPVTTSSSTSTLSSSPWSAQPTWKSDPGFRITVVTWNVGTAMPPDDVTSLLHLGGGDDSDGADMIAIGLQEVNSMLNKRLKDALFTDQWSELFMDALGPFNFVLVSSVRMQGVILLLFAKYYHLPFLRDVQTDCTRTGLGGYWGNKGGVSVRLAAFGHMLCFLNCHLPAHMDKAEQRKDNFQTILSLQQFQGPGAQGILDHDLVFWFGDLNFRIESYDLHFVKFAIDSDQLHQLWEKDQLNMAKNTWPILKGFQEGPLNFAPTFKFDVGTNKYDTSAKKRKPAWTDRILWKVKAPGGGPSPSGRKSHRLQVTQHSYRSHMEYTVSDHKPVAAQFLLQFAFRDDMPLVRLEVADEWVRPEQAVVRYRMETVFARSSWDWIGLYRVGFRHCKDYVAYVWAKHEDVDGNTYQVTFSEESLPKGHGDFILGYYSHNHSILIGITEPFQISLPSSELASSSTDSSGTSSEGEDDSTLELLAPKSRSPSPGKSKRHRSRSPGLARFPGLALRPSSRERRGASRSPSPQSRRLSRVAPDRSSNGSSRGSSEEGPSGLPGPWAFPPAVPRSLGLLPALRLETVDPGGGGSWGPDREALAPNSLSPSPQGHRGLEEGGLGP
种属预测
score>80的预测可信度较高，可尝试用于WB检测。*预测模型主要基于免疫原序列比对，结果仅作参考，不作为质保凭据。
High(score>80) Medium(80>score>50) Low(score<50) No confidence
翻译修饰 - Q15735 作为底物
| Site | PTM Type | Enzyme | Source |
|---|---|---|---|
| R7 | Methylation | Uniprot | |
| S20 | Phosphorylation | Uniprot | |
| T30 | Phosphorylation | Uniprot | |
| S34 | Phosphorylation | Uniprot | |
| S38 | Phosphorylation | Uniprot | |
| S39 | Phosphorylation | Uniprot | |
| R56 | Methylation | Uniprot | |
| R65 | Methylation | Uniprot | |
| R76 | Methylation | Uniprot | |
| S81 | Phosphorylation | Uniprot | |
| R83 | Methylation | Uniprot | |
| T91 | Phosphorylation | Uniprot | |
| S150 | Phosphorylation | Uniprot | |
| Y667 | Phosphorylation | Uniprot | |
| S694 | Phosphorylation | Uniprot | |
| S881 | Phosphorylation | Uniprot | |
| S886 | Phosphorylation | Uniprot | |
| S888 | Phosphorylation | Uniprot | |
| S902 | Phosphorylation | Uniprot | |
| S903 | Phosphorylation | Uniprot | |
| S910 | Phosphorylation | Uniprot | |
| S912 | Phosphorylation | Uniprot | |
| S914 | Phosphorylation | Uniprot | |
| S917 | Phosphorylation | Uniprot | |
| S990 | Phosphorylation | Uniprot | |
| S992 | Phosphorylation | Uniprot |
研究背景
Inositol 5-phosphatase, which converts inositol 1,4,5-trisphosphate to inositol 1,4-bisphosphate. Also converts phosphatidylinositol 4,5-bisphosphate to phosphatidylinositol 4-phosphate and inositol 1,3,4,5-tetrakisphosphate to inositol 1,3,4-trisphosphate in vitro. May be involved in modulation of the function of inositol and phosphatidylinositol polyphosphate-binding proteins that are present at membranes ruffles (By similarity).
Cytoplasm.
Note: Predominantly localized to membrane ruffles.
The 5 Arg-Ser-Xaa-Ser-Xaa-Xaa (RSXSXX) motifs may constitute binding sites for the 14-3-3 protein.
Belongs to the inositol 1,4,5-trisphosphate 5-phosphatase type II family.
研究领域
· Environmental Information Processing > Signal transduction > Phosphatidylinositol signaling system.
· Metabolism > Carbohydrate metabolism > Inositol phosphate metabolism.
· Metabolism > Global and overview maps > Metabolic pathways.
限制条款
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