产品描述
*The optimal dilutions should be determined by the end user.
*Tips:
WB: 适用于变性蛋白样本的免疫印迹检测. IHC: 适用于组织样本的石蜡(IHC-p)或冰冻(IHC-f)切片样本的免疫组化/荧光检测. IF/ICC: 适用于细胞样本的荧光检测. ELISA(peptide): 适用于抗原肽的ELISA检测.
引用格式: Affinity Biosciences Cat# DF9753, RRID:AB_2842948.
展开/折叠
Choline phosphatase 3; HindIII K4L homolog; HU K4; Hu-K4; HUK4; Phosphatidylcholine hydrolyzing phospholipase D3; Phosphatidylcholine-hydrolyzing phospholipase D3; Phospholipase D family, member 3; Phospholipase D3; PLD 3; PLD3; PLD3_HUMAN;
抗原和靶标
Widely expressed. In the brain, high levels of expression are detected in the frontal, temporal and occipital cortices and hippocampus. Expressed at low level in corpus callosum.
- Q8IV08 PLD3_HUMAN:
- Protein BLAST With
- NCBI/
- ExPASy/
- Uniprot
MKPKLMYQELKVPAEEPANELPMNEIEAWKAAEKKARWVLLVLILAVVGFGALMTQLFLWEYGDLHLFGPNQRPAPCYDPCEAVLVESIPEGLDFPNASTGNPSTSQAWLGLLAGAHSSLDIASFYWTLTNNDTHTQEPSAQQGEEVLRQLQTLAPKGVNVRIAVSKPSGPQPQADLQALLQSGAQVRMVDMQKLTHGVLHTKFWVVDQTHFYLGSANMDWRSLTQVKELGVVMYNCSCLARDLTKIFEAYWFLGQAGSSIPSTWPRFYDTRYNQETPMEICLNGTPALAYLASAPPPLCPSGRTPDLKALLNVVDNARSFIYVAVMNYLPTLEFSHPHRFWPAIDDGLRRATYERGVKVRLLISCWGHSEPSMRAFLLSLAALRDNHTHSDIQVKLFVVPADEAQARIPYARVNHNKYMVTERATYIGTSNWSGNYFTETAGTSLLVTQNGRGGLRSQLEAIFLRDWDSPYSHDLDTSADSVGNACRLL
种属预测
score>80的预测可信度较高，可尝试用于WB检测。*预测模型主要基于免疫原序列比对，结果仅作参考，不作为质保凭据。
High(score>80) Medium(80>score>50) Low(score<50) No confidence
翻译修饰 - Q8IV08 作为底物
| Site | PTM Type | Enzyme | Source |
|---|---|---|---|
| K4 | Ubiquitination | Uniprot | |
| Y7 | Phosphorylation | Uniprot | |
| K11 | Ubiquitination | Uniprot | |
| K30 | Ubiquitination | Uniprot | |
| N97 | N-Glycosylation | Uniprot | |
| N132 | N-Glycosylation | Uniprot | |
| K157 | Ubiquitination | Uniprot | |
| K167 | Ubiquitination | Uniprot | |
| S216 | Phosphorylation | Uniprot | |
| S223 | Phosphorylation | Uniprot | |
| T225 | Phosphorylation | Uniprot | |
| S238 | Phosphorylation | Uniprot | |
| K309 | Ubiquitination | Uniprot |
研究背景
5'->3' DNA exonuclease which digests single-stranded DNA (ssDNA). Regulates inflammatory cytokine responses via the degradation of nucleic acids, by reducing the concentration of ssDNA able to stimulate TLR9, a nucleotide-sensing receptor in collaboration with PLD4 (By similarity). May be important in myotube formation. Plays a role in lysosomal homeostasis. Involved in the regulation of endosomal protein sorting.
N-glycosylated.
Proteolytically processed to a soluble form that is stable within endosomes and lysosomes. During transport through the secretory pathway becomes proteolysed by cysteine proteases, thereby releasing a stable soluble lysosomal lumenal polypeptide, whereas the transmembrane-bound fragment is rapidly degraded. Its transport route to lysosomes involves ubiquitination and the ESCRT complex.
Ubiquitinated at N-terminus. Ubiquitination mediates sorting into lysosomes.
Endoplasmic reticulum membrane>Single-pass type II membrane protein. Lysosome lumen. Early endosome membrane>Single-pass type II membrane protein. Late endosome membrane>Single-pass type II membrane protein. Golgi apparatus membrane>Single-pass type II membrane protein. Endosome membrane>Single-pass type II membrane protein.
Note: Localizes to ER-associated vesicles in differentiating myotubes (PubMed:22428023). The soluble form in lysosome arises by proteolytic processing of the membrane-bound form (PubMed:29386126). Colocalizes with APP in endosomes (PubMed:29368044).
Widely expressed. In the brain, high levels of expression are detected in the frontal, temporal and occipital cortices and hippocampus. Expressed at low level in corpus callosum.
Interacts with APP.
Belongs to the phospholipase D family.
研究领域
· Metabolism > Lipid metabolism > Glycerophospholipid metabolism.
· Metabolism > Lipid metabolism > Ether lipid metabolism.
· Metabolism > Global and overview maps > Metabolic pathways.
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