产品描述
*The optimal dilutions should be determined by the end user.
*Tips:
WB: 适用于变性蛋白样本的免疫印迹检测. IHC: 适用于组织样本的石蜡(IHC-p)或冰冻(IHC-f)切片样本的免疫组化/荧光检测. IF/ICC: 适用于细胞样本的荧光检测. ELISA(peptide): 适用于抗原肽的ELISA检测.
引用格式: Affinity Biosciences Cat# DF9762, RRID:AB_2842957.
展开/折叠
Activated platelet protein 1; Activated-platelet protein 1; APP 1; APP-1; APP1; FLJ43938; Inducible poly(A) binding protein; Inducible poly(A)-binding protein; iPABP; IPABP4; PABP 4; PABP-4; PABP4; PABP4_HUMAN; PABPC 4; PABPC4; Poly A binding protein cytoplasmic 4; Poly(A) binding protein 4; Poly(A) binding protein cytoplasmic 4 (inducible form); Poly(A) binding protein cytoplasmic 4; Poly(A)-binding protein 4; Polyadenylate binding protein 4; Polyadenylate-binding protein 4; Polyadenylate-binding protein, cytoplasmic, 4; Polyadenylate-binding protein, inducible;
抗原和靶标
Expressed at low levels in resting normal T cells; following T-cell activation, however, mRNA levels are rapidly up-regulated.
- Q13310 PABP4_HUMAN:
- Protein BLAST With
- NCBI/
- ExPASy/
- Uniprot
MNAAASSYPMASLYVGDLHSDVTEAMLYEKFSPAGPVLSIRVCRDMITRRSLGYAYVNFQQPADAERALDTMNFDVIKGKPIRIMWSQRDPSLRKSGVGNVFIKNLDKSIDNKALYDTFSAFGNILSCKVVCDENGSKGYAFVHFETQEAADKAIEKMNGMLLNDRKVFVGRFKSRKEREAELGAKAKEFTNVYIKNFGEEVDDESLKELFSQFGKTLSVKVMRDPNGKSKGFGFVSYEKHEDANKAVEEMNGKEISGKIIFVGRAQKKVERQAELKRKFEQLKQERISRYQGVNLYIKNLDDTIDDEKLRKEFSPFGSITSAKVMLEDGRSKGFGFVCFSSPEEATKAVTEMNGRIVGSKPLYVALAQRKEERKAHLTNQYMQRVAGMRALPANAILNQFQPAAGGYFVPAVPQAQGRPPYYTPNQLAQMRPNPRWQQGGRPQGFQGMPSAIRQSGPRPTLRHLAPTGSECPDRLAMDFGGAGAAQQGLTDSCQSGGVPTAVQNLAPRAAVAAAAPRAVAPYKYASSVRSPHPAIQPLQAPQPAVHVQGQEPLTASMLAAAPPQEQKQMLGERLFPLIQTMHSNLAGKITGMLLEIDNSELLHMLESPESLRSKVDEAVAVLQAHHAKKEAAQKVGAVAAATS
种属预测
score>80的预测可信度较高，可尝试用于WB检测。*预测模型主要基于免疫原序列比对，结果仅作参考，不作为质保凭据。
High(score>80) Medium(80>score>50) Low(score<50) No confidence
翻译修饰 - Q13310 作为底物
| Site | PTM Type | Enzyme | Source |
|---|---|---|---|
| S32 | Phosphorylation | Uniprot | |
| S39 | Phosphorylation | Uniprot | |
| S51 | Phosphorylation | Uniprot | |
| Y54 | Phosphorylation | Uniprot | |
| Y56 | Phosphorylation | Uniprot | |
| T71 | Phosphorylation | Uniprot | |
| K78 | Ubiquitination | Uniprot | |
| K80 | Ubiquitination | Uniprot | |
| S92 | Phosphorylation | Uniprot | |
| K95 | Ubiquitination | Uniprot | |
| S96 | Phosphorylation | Uniprot | |
| K104 | Acetylation | Uniprot | |
| K104 | Ubiquitination | Uniprot | |
| K108 | Acetylation | Uniprot | |
| K108 | Ubiquitination | Uniprot | |
| K113 | Ubiquitination | Uniprot | |
| Y116 | Phosphorylation | Uniprot | |
| S120 | Phosphorylation | Uniprot | |
| S127 | Phosphorylation | Uniprot | |
| K129 | Ubiquitination | Uniprot | |
| K138 | Ubiquitination | Uniprot | |
| Y140 | Phosphorylation | Uniprot | |
| K157 | Ubiquitination | Uniprot | |
| R166 | Methylation | Uniprot | |
| K167 | Sumoylation | Uniprot | |
| K167 | Ubiquitination | Uniprot | |
| K177 | Ubiquitination | Uniprot | |
| R179 | Methylation | Uniprot | |
| K186 | Ubiquitination | Uniprot | |
| K188 | Methylation | Uniprot | |
| K188 | Ubiquitination | Uniprot | |
| T191 | Phosphorylation | Uniprot | |
| Y194 | Phosphorylation | Uniprot | |
| K196 | Ubiquitination | Uniprot | |
| S206 | Phosphorylation | Uniprot | |
| K208 | Ubiquitination | Uniprot | |
| K216 | Ubiquitination | Uniprot | |
| K221 | Ubiquitination | Uniprot | |
| K240 | Ubiquitination | Uniprot | |
| K254 | Ubiquitination | Uniprot | |
| S257 | Phosphorylation | Uniprot | |
| K259 | Ubiquitination | Uniprot | |
| K279 | Ubiquitination | Uniprot | |
| K284 | Ubiquitination | Uniprot | |
| Y291 | Phosphorylation | Uniprot | |
| Y297 | Phosphorylation | Uniprot | |
| K299 | Acetylation | Uniprot | |
| K299 | Ubiquitination | Uniprot | |
| T304 | Phosphorylation | Uniprot | |
| K309 | Ubiquitination | Uniprot | |
| K312 | Ubiquitination | Uniprot | |
| S315 | Phosphorylation | Uniprot | |
| S319 | Phosphorylation | Uniprot | |
| T321 | Phosphorylation | Uniprot | |
| S322 | Phosphorylation | Uniprot | |
| K324 | Ubiquitination | Uniprot | |
| K333 | Ubiquitination | Uniprot | |
| S341 | Phosphorylation | Uniprot | |
| S342 | Phosphorylation | Uniprot | |
| K348 | Ubiquitination | Uniprot | |
| S360 | Phosphorylation | Uniprot | |
| K361 | Ubiquitination | Uniprot | |
| Y364 | Phosphorylation | Uniprot | |
| K375 | Methylation | Uniprot | |
| K375 | Ubiquitination | Uniprot | |
| T379 | Phosphorylation | Uniprot | |
| Y382 | Phosphorylation | Uniprot | |
| R385 | Methylation | Uniprot | |
| R419 | Methylation | Uniprot | |
| R432 | Methylation | Uniprot | |
| R436 | Methylation | Uniprot | |
| R442 | Methylation | Uniprot | |
| R454 | Methylation | Uniprot | |
| R459 | Methylation | Uniprot | |
| R463 | Methylation | Uniprot | |
| R509 | Methylation | Uniprot | |
| R518 | Methylation | Uniprot | |
| K524 | Methylation | Uniprot | |
| R530 | Methylation | Uniprot | |
| S531 | Phosphorylation | Uniprot | |
| S584 | Phosphorylation | Uniprot | |
| S600 | Phosphorylation | Uniprot | |
| S608 | Phosphorylation | Uniprot | |
| K629 | Ubiquitination | Uniprot | |
| K635 | Ubiquitination | Uniprot | |
| S644 | Phosphorylation | Uniprot |
研究背景
Binds the poly(A) tail of mRNA. May be involved in cytoplasmic regulatory processes of mRNA metabolism. Can probably bind to cytoplasmic RNA sequences other than poly(A) in vivo (By similarity).
Arg-518 is dimethylated, probably to asymmetric dimethylarginine.
Cytoplasm.
Note: Localized in cytoplasmic mRNP granules containing untranslated mRNAs.
Expressed at low levels in resting normal T cells; following T-cell activation, however, mRNA levels are rapidly up-regulated.
Identified in a IGF2BP1-dependent mRNP granule complex containing untranslated mRNAs. Interacts with NFX1. Interacts with ZFC3H1 in a RNase-sensitive manner.
Belongs to the polyadenylate-binding protein type-1 family.
研究领域
· Genetic Information Processing > Translation > RNA transport.
· Genetic Information Processing > Translation > mRNA surveillance pathway.
· Genetic Information Processing > Folding, sorting and degradation > RNA degradation.
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