产品描述
*The optimal dilutions should be determined by the end user.
*Tips:
WB: 适用于变性蛋白样本的免疫印迹检测. IHC: 适用于组织样本的石蜡(IHC-p)或冰冻(IHC-f)切片样本的免疫组化/荧光检测. IF/ICC: 适用于细胞样本的荧光检测. ELISA(peptide): 适用于抗原肽的ELISA检测.
引用格式: Affinity Biosciences Cat# DF9780, RRID:AB_2842975.
展开/折叠
PP1 subunit R6; PPP1R3D; PPP1R6; PPR3D_HUMAN; Protein phosphatase 1 regulatory subunit 3D; Protein phosphatase 1 regulatory subunit 6; Protein phosphatase 1-binding subunit R6;
抗原和靶标
- O95685 PPR3D_HUMAN:
- Protein BLAST With
- NCBI/
- ExPASy/
- Uniprot
MSRGPSSAVLPSALGSRKLGPRSLSCLSDLDGGVALEPRACRPPGSPGRAPPPTPAPSGCDPRLRPIILRRARSLPSSPERRQKAAGAPGAACRPGCSQKLRVRFADALGLELAQVKVFNAGDDPSVPLHVLSRLAINSDLCCSSQDLEFTLHCLVPDFPPPVEAADFGERLQRQLVCLERVTCSDLGISGTVRVCNVAFEKQVAVRYTFSGWRSTHEAVARWRGPAGPEGTEDVFTFGFPVPPFLLELGSRVHFAVRYQVAGAEYWDNNDHRDYSLTCRNHALHMPRGECEESWIHFI
种属预测
score>80的预测可信度较高，可尝试用于WB检测。*预测模型主要基于免疫原序列比对，结果仅作参考，不作为质保凭据。
High(score>80) Medium(80>score>50) Low(score<50) No confidence
翻译修饰 - O95685 作为底物
| Site | PTM Type | Enzyme | Source |
|---|---|---|---|
| R3 | Methylation | Uniprot | |
| S6 | Phosphorylation | Uniprot | |
| S7 | Phosphorylation | Uniprot | |
| S16 | Phosphorylation | Uniprot | |
| S23 | Phosphorylation | Uniprot | |
| S25 | Phosphorylation | Uniprot | |
| S28 | Phosphorylation | Uniprot | |
| S46 | Phosphorylation | Uniprot | |
| T54 | Phosphorylation | Uniprot | |
| S74 | Phosphorylation | Uniprot | |
| S77 | Phosphorylation | Uniprot | |
| S78 | Phosphorylation | Uniprot | |
| S98 | Phosphorylation | Uniprot | |
| K100 | Ubiquitination | Uniprot | |
| S133 | Phosphorylation | Uniprot |
研究背景
Seems to act as a glycogen-targeting subunit for PP1. PP1 is essential for cell division, and participates in the regulation of glycogen metabolism, muscle contractility and protein synthesis.
Expressed in all tissues tested. High expression in skeletal muscle and heart.
Interacts with PPP1CC catalytic subunit of PP1, and associates with glycogen. Interacts with EPM2A; in the presence of NHLC1/malin the interaction leads to PPP1R3D ubiquitination and autophagic degradation.
The CBM21 domain is known to be involved in the localization to glycogen and is characteristic of some regulatory subunit of phosphatase complexes.
研究领域
· Human Diseases > Endocrine and metabolic diseases > Insulin resistance.
· Organismal Systems > Endocrine system > Insulin signaling pathway. (View pathway)
限制条款
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