| 产品: | RBM16 抗体 |
| 货号: | DF9790 |
| 描述: | Rabbit polyclonal antibody to RBM16 |
| 应用: | WB IF/ICC |
| 反应: | Human, Mouse, Rat |
| 预测: | Pig, Bovine, Horse, Sheep, Rabbit, Dog, Chicken |
| 分子量: | 141kDa; 141kD(Calculated). |
| 蛋白号: | Q9UPN6 |
| RRID: | AB_2842985 |
产品描述
*The optimal dilutions should be determined by the end user.
*Tips:
WB: 适用于变性蛋白样本的免疫印迹检测. IHC: 适用于组织样本的石蜡(IHC-p)或冰冻(IHC-f)切片样本的免疫组化/荧光检测. IF/ICC: 适用于细胞样本的荧光检测. ELISA(peptide): 适用于抗原肽的ELISA检测.
引用格式: Affinity Biosciences Cat# DF9790, RRID:AB_2842985.
展开/折叠
CDC5L complex associated protein 7; CDC5L complex-associated protein 7; Protein SCAF8; putative RNA binding protein 16; RBM 16; RNA binding motif protein 16; RNA binding protein 16; RNA-binding motif protein 16; SCAF 8; SCAF8; SCAF8_HUMAN; SR like CTD associated factor 8; SR related and CTD associated factor 8; SR-related and CTD-associated factor 8;
抗原和靶标
- Q9UPN6 SCAF8_HUMAN:
- Protein BLAST With
- NCBI/
- ExPASy/
- Uniprot
MEAVKTFNSELYSLNDYKPPISKAKMTQITKAAIKAIKFYKHVVQSVEKFIQKCKPEYKVPGLYVIDSIVRQSRHQFGQEKDVFAPRFSNNIISTFQNLYRCPGDDKSKIVRVLNLWQKNNVFKSEIIQPLLDMAAGIPPPVVTPVLASTTTAMSNTPGTPVTPVTPANVVQGLPDPWVSQITNTDTLAAVAQILQSPQGQQLQQLIQTLQIQQQKPQPSILQALDAGLVVQLQALTAQLTAAAAAANTLTPLEQGVSFNKKLMDRFDFGEDSEHSEEPKKEIPASQLSHVSESVNNSIFHQIAEQLQQQNLEHLRQQLLEQQQPQKATPQDSQEGTFGSEHSASPSQGSSQQHFLEPEVNLDDSIDIQQQDMDIDEGQDGVEEEVFEQEAKKVAVRSRSRTHSRSRSRSPRKRRSRSRSGSRKRKHRKRSRSRSRERKRKSSRSYSSERRAREREKERQKKGLPPIRSKTLSVCSTTLWVGQVDKKATQQDLTNLFEEFGQIESINMIPPRGCAYVCMVHRQDAFRALQKLSSGSYKIGSKVIKIAWALNKGVKTEYKQFWDVDLGVTYIPWEKVKVDDLEGFAEGGMIDQETVNTEWETVKSSEPVKETVQTTQSPTPVEKETVVTTQAEVFPPPVAMLQIPVAPAVPTVSLVPPAFPVSMPVPPPGFSPIPPPPFLRASFNPSQPPPGFMPPPVPPPVVPPPTIPPVVPTSLVQPSLSMTPETVKDVGFGSLVIPGGSVASNLATSALPAGNVFNAPTKQAEPEEKVPHLIDHQISSGENTRSVIPNDISSNAAILGGQPPNVTSNSGILGVQRPNVSSNSEILGVRPSNVSSSSGIIAAQPPNILNNSGILGIQPPSVSNSSGLLGVLPPNIPNNSGLVGVQPPNVPNTPGLLGTQPPAGPQNLPPLSIPNQRMPTMPMLDIRPGLIPQAPGPRFPLIQPGIPPQRGIPPPSVLDSALHPPPRGPFPPGDIFSQPERPFLAPGRQSVDNVTNPEKRIPLGNDNIQQEGDRDYRFPPIETRESISRPPPVDVRDVVGRPIDPREGPGRPPLDGRDHFGRPPVDIRENLVRPGIDHLGRRDHFGFNPEKPWGHRGDFDEREHRVLPVYGGPKGLHEERGRFRSGNYRFDPRSGPWNRGFGQEVHRDFDDRRRPWERQRDRDDRDFDFCREMNGNRLGRDRIQNTWVPPPHARVFDYFEGATSQRKGDNVPQVNGENTERHAQPPPIPVQNDPELYEKLTSSNEINKEKSDTVADIESEPVVESTETEGT
种属预测
score>80的预测可信度较高，可尝试用于WB检测。*预测模型主要基于免疫原序列比对，结果仅作参考，不作为质保凭据。
High(score>80) Medium(80>score>50) Low(score<50) No confidence
翻译修饰 - Q9UPN6 作为底物
| Site | PTM Type | Enzyme | Source |
|---|---|---|---|
| T6 | Phosphorylation | Uniprot | |
| Y12 | Phosphorylation | Uniprot | |
| Y17 | Phosphorylation | Uniprot | |
| K18 | Sumoylation | Uniprot | |
| K18 | Ubiquitination | Uniprot | |
| S22 | Phosphorylation | Uniprot | |
| K31 | Ubiquitination | Uniprot | |
| K53 | Ubiquitination | Uniprot | |
| K81 | Ubiquitination | Uniprot | |
| K119 | Ubiquitination | Uniprot | |
| K124 | Ubiquitination | Uniprot | |
| S273 | Phosphorylation | Uniprot | |
| K281 | Ubiquitination | Uniprot | |
| S443 | Phosphorylation | Uniprot | |
| S445 | Phosphorylation | Uniprot | |
| S447 | Phosphorylation | Uniprot | |
| K461 | Acetylation | Uniprot | |
| S536 | Phosphorylation | Uniprot | |
| K542 | Ubiquitination | Uniprot | |
| K609 | Acetylation | Uniprot | |
| T611 | Phosphorylation | Uniprot | |
| T614 | O-Glycosylation | Uniprot | |
| T615 | O-Glycosylation | Uniprot | |
| T615 | Phosphorylation | Uniprot | |
| S617 | Phosphorylation | Uniprot | |
| T619 | Phosphorylation | Uniprot | |
| S779 | Phosphorylation | Uniprot | |
| S780 | Phosphorylation | Uniprot | |
| R917 | Methylation | Uniprot | |
| R927 | Methylation | Uniprot | |
| R938 | Methylation | Uniprot | |
| R967 | Methylation | Uniprot | |
| R981 | Methylation | Uniprot | |
| R988 | Methylation | Uniprot | |
| R1062 | Methylation | Uniprot | |
| R1073 | Methylation | Uniprot | |
| K1091 | Ubiquitination | Uniprot | |
| R1120 | Methylation | Uniprot | |
| R1122 | Methylation | Uniprot | |
| R1124 | Methylation | Uniprot | |
| R1129 | Methylation | Uniprot | |
| R1133 | Methylation | Uniprot | |
| R1139 | Methylation | Uniprot | |
| Y1198 | Phosphorylation | Uniprot | |
| Y1237 | Phosphorylation | Uniprot |
研究背景
Anti-terminator protein required to prevent early mRNA termination during transcription. Together with SCAF4, acts by suppressing the use of early, alternative poly(A) sites, thereby preventing the accumulation of non-functional truncated proteins. Mechanistically, associates with the phosphorylated C-terminal heptapeptide repeat domain (CTD) of the largest RNA polymerase II subunit (POLR2A), and subsequently binds nascent RNA upstream of early polyadenylation sites to prevent premature mRNA transcript cleavage and polyadenylation. Independently of SCAF4, also acts as a positive regulator of transcript elongation.
Nucleus. Nucleus matrix.
Note: Detected in granular nuclear foci which correspond to sites of active transcription.
Interacts with POLR2A; via C-terminal heptapeptide repeat domain (CTD) phosphorylated at 'Ser-2' and 'Ser-5'. Identified in a complex with CDC5L and other spliceosomal proteins.
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