产品: RAB1A 抗体
货号: DF9817
描述: Rabbit polyclonal antibody to RAB1A
应用: WB IHC IF/ICC
反应: Human, Mouse, Rat
预测: Pig, Bovine, Horse, Sheep, Rabbit, Dog, Chicken
分子量: 23 kDa; 23kD(Calculated).
蛋白号: P62820
RRID: AB_2843011

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   规格 价格 库存
 100ul RMB¥ 2300 现货
 200ul RMB¥ 3000 现货

货期: 当天发货

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产品描述

来源:
Rabbit
应用:
IHC 1:50-1:200, WB 1:1000-3000, IF/ICC 1:100-1:500
*The optimal dilutions should be determined by the end user.
*Tips:

WB: 适用于变性蛋白样本的免疫印迹检测. IHC: 适用于组织样本的石蜡(IHC-p)或冰冻(IHC-f)切片样本的免疫组化/荧光检测. IF/ICC: 适用于细胞样本的荧光检测. ELISA(peptide): 适用于抗原肽的ELISA检测.

反应:
Human, Mouse, Rat
预测:
Pig(100%), Bovine(%), Horse(%), Sheep(%), Rabbit(%), Dog(%), Chicken(%)
克隆:
Polyclonal
特异性:
RAB1A Antibody detects endogenous levels of total RAB1A.
RRID:
AB_2843011
引用格式: Affinity Biosciences Cat# DF9817, RRID:AB_2843011.
偶联:
Unconjugated. 130
纯化:
The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).
保存:
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
别名:

展开/折叠

GTP binding protein RAB 1A; mKIAA3012; RAB 1; Rab 1A; RAB1; RAB1, member RAS oncogene family; Rab1A; RAB1A member RAS oncogene family; RAB1A_HUMAN; Ras related protein Rab 1A; Ras-associated protein RAB1; Ras-related protein Rab-1A; YPT1; YPT1 related protein; YPT1-related protein;

抗原和靶标

免疫原:
Uniprot:
基因/基因ID:
序列:
MSSMNPEYDYLFKLLLIGDSGVGKSCLLLRFADDTYTESYISTIGVDFKIRTIELDGKTIKLQIWDTAGQERFRTITSSYYRGAHGIIVVYDVTDQESFNNVKQWLQEIDRYASENVNKLLVGNKCDLTTKKVVDYTTAKEFADSLGIPFLETSAKNATNVEQSFMTMAAEIKKRMGPGATAGGAEKSNVKIQSTPVKQSGGGCC

种属预测

种属预测:

score>80的预测可信度较高,可尝试用于WB检测。*预测模型主要基于免疫原序列比对,结果仅作参考,不作为质保凭据。

Species
Results
Score
Pig
100
Horse
100
Bovine
100
Sheep
100
Dog
100
Chicken
100
Rabbit
100
Xenopus
67
Zebrafish
0
Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

翻译修饰 - P62820 作为底物

Site PTM Type Enzyme
S2 Acetylation
Y8 Phosphorylation
Y10 Phosphorylation
S20 Phosphorylation
Y36 Phosphorylation
S39 Phosphorylation
Y40 Phosphorylation
T52 Phosphorylation
K58 Ubiquitination
K61 Acetylation
T67 Phosphorylation
T75 Phosphorylation
T77 Phosphorylation
Y80 Phosphorylation
Y81 Phosphorylation
T94 Phosphorylation
K103 Ubiquitination
R111 Methylation
S114 Phosphorylation
K119 Ubiquitination
K125 Ubiquitination
C126 S-Nitrosylation
K132 Ubiquitination
Y136 Phosphorylation
S145 Phosphorylation
K173 Ubiquitination
T181 Phosphorylation
K187 Ubiquitination
S188 Phosphorylation
K191 Ubiquitination
S194 Phosphorylation
T195 Phosphorylation

研究背景

功能:

The small GTPases Rab are key regulators of intracellular membrane trafficking, from the formation of transport vesicles to their fusion with membranes. Rabs cycle between an inactive GDP-bound form and an active GTP-bound form that is able to recruit to membranes different sets of downstream effectors directly responsible for vesicle formation, movement, tethering and fusion. RAB1A regulates vesicular protein transport from the endoplasmic reticulum (ER) to the Golgi compartment and on to the cell surface, and plays a role in IL-8 and growth hormone secretion. Regulates the level of CASR present at the cell membrane. Plays a role in cell adhesion and cell migration, via its role in protein trafficking. Plays a role in autophagosome assembly and cellular defense reactions against pathogenic bacteria. Plays a role in microtubule-dependent protein transport by early endosomes and in anterograde melanosome transport.

翻译修饰:

Phosphorylated by CDK1 kinase during mitosis.

Phosphocholinated at Ser-79 by L.pneumophila AnkX, leading to displace GDP dissociation inhibitors (GDI). Both GDP-bound and GTP-bound forms can be phosphocholinated. Dephosphocholinated by L.pneumophila Lem3, restoring accessibility to L.pneumophila GTPase effector LepB.

细胞定位:

Golgi apparatus. Endoplasmic reticulum. Early endosome. Cytoplasm>Cytosol. Membrane. Melanosome.
Note: Alternates between membrane-associated and cytosolic forms.

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionSubcellular location
亚基结构:

May interact with YIPF5 (By similarity). Interacts with C9orf72; the interaction mediates recruitment of RAB1A to the ATG1/ULK1 kinase complex. Interacts with GDI1; this promotes dissociation from membranes.

(Microbial infection) Interacts with L.pneumophila AnkX.

(Microbial infection) Interacts with L.pneumophila Lem3.

(Microbial infection) Interacts with L.pneumophila LidA.

(Microbial infection) Interacts with L.pneumophila DrrA; this disrupts the interaction between RAB1A and GDI1 and promotes the exchange of RAB1A-bound GDP with GTP.

(Microbial infection) Interacts with E.coli EspG and S.flexneri VirA; this impairs ER to Golgi trafficking and protein secretion.

(Microbial infection) Interacts with L.pneumophila and L.drancourtii LepB; this enhances RAB1A GTPase activity.

(Microbial infection) Identified in a complex composed of RAB1A, ARF6 and E.coli EspG.

蛋白家族:

Belongs to the small GTPase superfamily. Rab family.

研究领域

· Human Diseases > Infectious diseases: Bacterial > Legionellosis.

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