产品: RAB5B 抗体
货号: DF9834
描述: Rabbit polyclonal antibody to RAB5B
应用: WB IHC
反应: Human, Mouse, Rat
预测: Pig, Bovine, Horse, Sheep, Rabbit, Dog
分子量: 24 kDa; 24kD(Calculated).
蛋白号: P61020
RRID: AB_2843028

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   规格 价格 库存
 100ul RMB¥ 2300 现货
 200ul RMB¥ 3000 现货

货期: 当天发货

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产品描述

来源:
Rabbit
应用:
IHC 1:50-1:200, WB 1:1000-3000
*The optimal dilutions should be determined by the end user.
*Tips:

WB: 适用于变性蛋白样本的免疫印迹检测. IHC: 适用于组织样本的石蜡(IHC-p)或冰冻(IHC-f)切片样本的免疫组化/荧光检测. IF/ICC: 适用于细胞样本的荧光检测. ELISA(peptide): 适用于抗原肽的ELISA检测.

反应:
Human,Mouse,Rat
预测:
Pig(100%), Bovine(100%), Horse(91%), Sheep(100%), Rabbit(100%), Dog(100%)
克隆:
Polyclonal
特异性:
RAB5B Antibody detects endogenous levels of total RAB5B.
RRID:
AB_2843028
引用格式: Affinity Biosciences Cat# DF9834, RRID:AB_2843028.
偶联:
Unconjugated.
纯化:
The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).
保存:
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
别名:

展开/折叠

Rab 5b; Rab5 b; RAB5B, member RAS oncogene family; Ras related protein Rab 5B; RAS-associated protein RAB5B;

抗原和靶标

免疫原:
Uniprot:
基因/基因ID:
序列:
MTSRSTARPNGQPQASKICQFKLVLLGESAVGKSSLVLRFVKGQFHEYQESTIGAAFLTQSVCLDDTTVKFEIWDTAGQERYHSLAPMYYRGAQAAIVVYDITNQETFARAKTWVKELQRQASPSIVIALAGNKADLANKRMVEYEEAQAYADDNSLLFMETSAKTAMNVNDLFLAIAKKLPKSEPQNLGGAAGRSRGVDLHEQSQQNKSQCCSN

种属预测

种属预测:

score>80的预测可信度较高,可尝试用于WB检测。*预测模型主要基于免疫原序列比对,结果仅作参考,不作为质保凭据。

Species
Results
Score
Pig
100
Bovine
100
Sheep
100
Dog
100
Rabbit
100
Horse
91
Chicken
73
Xenopus
0
Zebrafish
0
Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

翻译修饰 - P61020 作为底物

Site PTM Type Enzyme
T2 Acetylation
T6 Phosphorylation Q5S007 (LRRK2)
K17 Ubiquitination
K22 Acetylation
S29 Phosphorylation
K33 Ubiquitination
Y82 Phosphorylation
S84 Phosphorylation
K116 Ubiquitination
S123 Phosphorylation P06493 (CDK1)
S125 Phosphorylation
K134 Ubiquitination
K140 Ubiquitination
S184 Phosphorylation
S205 Phosphorylation

研究背景

功能:

Protein transport. Probably involved in vesicular traffic (By similarity).

翻译修饰:

Phosphorylation of Ser-84 in the switch II region by LRRK2 prevents the association of RAB regulatory proteins, including CHM, CHML and RAB GDP dissociation inhibitors GDI1 and GDI2.

细胞定位:

Cell membrane>Lipid-anchor>Cytoplasmic side. Early endosome membrane>Lipid-anchor. Melanosome.
Note: Enriched in stage I melanosomes.

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionSubcellular location
亚基结构:

Binds EEA1. Interacts with RIN2 and RIN3, which probably regulate its pathway, possibly by acting as GEFs. Interacts with GDI1, GDI2, CHML and CHM; phosphorylation at Ser-84 disrupts this interaction.

蛋白家族:

Belongs to the small GTPase superfamily. Rab family.

研究领域

· Cellular Processes > Transport and catabolism > Endocytosis.   (View pathway)

· Cellular Processes > Transport and catabolism > Phagosome.   (View pathway)

· Environmental Information Processing > Signal transduction > Ras signaling pathway.   (View pathway)

· Human Diseases > Infectious diseases: Parasitic > Amoebiasis.

· Human Diseases > Infectious diseases: Bacterial > Tuberculosis.

· Organismal Systems > Excretory system > Vasopressin-regulated water reabsorption.

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