| 产品: | RAB5B 抗体 |
| 货号: | DF9834 |
| 描述: | Rabbit polyclonal antibody to RAB5B |
| 应用: | WB IHC |
| 反应: | Human, Mouse, Rat |
| 预测: | Pig, Bovine, Horse, Sheep, Rabbit, Dog |
| 分子量: | 24 kDa; 24kD(Calculated). |
| 蛋白号: | P61020 |
| RRID: | AB_2843028 |
产品描述
*The optimal dilutions should be determined by the end user.
*Tips:
WB: 适用于变性蛋白样本的免疫印迹检测. IHC: 适用于组织样本的石蜡(IHC-p)或冰冻(IHC-f)切片样本的免疫组化/荧光检测. IF/ICC: 适用于细胞样本的荧光检测. ELISA(peptide): 适用于抗原肽的ELISA检测.
引用格式: Affinity Biosciences Cat# DF9834, RRID:AB_2843028.
展开/折叠
Rab 5b; Rab5 b; RAB5B, member RAS oncogene family; Ras related protein Rab 5B; RAS-associated protein RAB5B;
抗原和靶标
- P61020 RAB5B_HUMAN:
- Protein BLAST With
- NCBI/
- ExPASy/
- Uniprot
MTSRSTARPNGQPQASKICQFKLVLLGESAVGKSSLVLRFVKGQFHEYQESTIGAAFLTQSVCLDDTTVKFEIWDTAGQERYHSLAPMYYRGAQAAIVVYDITNQETFARAKTWVKELQRQASPSIVIALAGNKADLANKRMVEYEEAQAYADDNSLLFMETSAKTAMNVNDLFLAIAKKLPKSEPQNLGGAAGRSRGVDLHEQSQQNKSQCCSN
种属预测
score>80的预测可信度较高，可尝试用于WB检测。*预测模型主要基于免疫原序列比对，结果仅作参考，不作为质保凭据。
High(score>80) Medium(80>score>50) Low(score<50) No confidence
翻译修饰 - P61020 作为底物
| Site | PTM Type | Enzyme | Source |
|---|---|---|---|
| T2 | Acetylation | Uniprot | |
| T6 | Phosphorylation | Q5S007 (LRRK2) | Uniprot |
| K17 | Ubiquitination | Uniprot | |
| K22 | Acetylation | Uniprot | |
| S29 | Phosphorylation | Uniprot | |
| K33 | Ubiquitination | Uniprot | |
| Y82 | Phosphorylation | Uniprot | |
| S84 | Phosphorylation | Uniprot | |
| K116 | Ubiquitination | Uniprot | |
| S123 | Phosphorylation | P06493 (CDK1) | Uniprot |
| S125 | Phosphorylation | Uniprot | |
| K134 | Ubiquitination | Uniprot | |
| K140 | Ubiquitination | Uniprot | |
| S184 | Phosphorylation | Uniprot | |
| S205 | Phosphorylation | Uniprot |
研究背景
Protein transport. Probably involved in vesicular traffic (By similarity).
Phosphorylation of Ser-84 in the switch II region by LRRK2 prevents the association of RAB regulatory proteins, including CHM, CHML and RAB GDP dissociation inhibitors GDI1 and GDI2.
Cell membrane>Lipid-anchor>Cytoplasmic side. Early endosome membrane>Lipid-anchor. Melanosome.
Note: Enriched in stage I melanosomes.
Binds EEA1. Interacts with RIN2 and RIN3, which probably regulate its pathway, possibly by acting as GEFs. Interacts with GDI1, GDI2, CHML and CHM; phosphorylation at Ser-84 disrupts this interaction.
Belongs to the small GTPase superfamily. Rab family.
研究领域
· Cellular Processes > Transport and catabolism > Endocytosis. (View pathway)
· Cellular Processes > Transport and catabolism > Phagosome. (View pathway)
· Environmental Information Processing > Signal transduction > Ras signaling pathway. (View pathway)
· Human Diseases > Infectious diseases: Parasitic > Amoebiasis.
· Human Diseases > Infectious diseases: Bacterial > Tuberculosis.
· Organismal Systems > Excretory system > Vasopressin-regulated water reabsorption.
限制条款
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