| 产品: | RAB8B 抗体 |
| 货号: | DF9837 |
| 描述: | Rabbit polyclonal antibody to RAB8B |
| 应用: | WB IHC |
| 反应: | Human, Mouse, Rat |
| 预测: | Pig, Bovine, Horse, Sheep, Rabbit, Chicken |
| 分子量: | 24 kDa; 24kD(Calculated). |
| 蛋白号: | Q92930 |
| RRID: | AB_2843031 |
产品描述
*The optimal dilutions should be determined by the end user.
*Tips:
WB: 适用于变性蛋白样本的免疫印迹检测. IHC: 适用于组织样本的石蜡(IHC-p)或冰冻(IHC-f)切片样本的免疫组化/荧光检测. IF/ICC: 适用于细胞样本的荧光检测. ELISA(peptide): 适用于抗原肽的ELISA检测.
引用格式: Affinity Biosciences Cat# DF9837, RRID:AB_2843031.
展开/折叠
5930437D16; D330025I23Rik; FLJ38125; GTPase Rab8b; RAB 8b protein; RAB44; Rab8b; RAB8B, member RAS oncogene family; RAB8B_HUMAN; Ras-related protein Rab-8B;
抗原和靶标
- Q92930 RAB8B_HUMAN:
- Protein BLAST With
- NCBI/
- ExPASy/
- Uniprot
MAKTYDYLFKLLLIGDSGVGKTCLLFRFSEDAFNTTFISTIGIDFKIRTIELDGKKIKLQIWDTAGQERFRTITTAYYRGAMGIMLVYDITNEKSFDNIKNWIRNIEEHASSDVERMILGNKCDMNDKRQVSKERGEKLAIDYGIKFLETSAKSSANVEEAFFTLARDIMTKLNRKMNDSNSAGAGGPVKITENRSKKTSFFRCSLL
种属预测
score>80的预测可信度较高，可尝试用于WB检测。*预测模型主要基于免疫原序列比对，结果仅作参考，不作为质保凭据。
High(score>80) Medium(80>score>50) Low(score<50) No confidence
翻译修饰 - Q92930 作为底物
| Site | PTM Type | Enzyme | Source |
|---|---|---|---|
| K3 | Acetylation | Uniprot | |
| K3 | Ubiquitination | Uniprot | |
| T4 | Phosphorylation | Uniprot | |
| Y5 | Phosphorylation | Uniprot | |
| Y7 | Phosphorylation | Uniprot | |
| S17 | Phosphorylation | Uniprot | |
| T49 | Phosphorylation | Uniprot | |
| T64 | Phosphorylation | Uniprot | |
| T72 | Phosphorylation | Uniprot | |
| Y77 | Phosphorylation | Uniprot | |
| K100 | Ubiquitination | Uniprot | |
| S111 | Phosphorylation | Uniprot | |
| S112 | Phosphorylation | Uniprot | |
| K122 | Ubiquitination | Uniprot | |
| S132 | Phosphorylation | Uniprot | |
| Y143 | Phosphorylation | Uniprot | |
| K153 | Ubiquitination | Uniprot | |
| T164 | Phosphorylation | Uniprot | |
| K176 | Ubiquitination | Uniprot | |
| S180 | Phosphorylation | Uniprot | |
| S182 | Phosphorylation | Uniprot | |
| K190 | Ubiquitination | Uniprot | |
| S200 | Phosphorylation | Uniprot |
研究背景
The small GTPases Rab are key regulators of intracellular membrane trafficking, from the formation of transport vesicles to their fusion with membranes. Rabs cycle between an inactive GDP-bound form and an active GTP-bound form that is able to recruit to membranes different sets of downstream effectors directly responsible for vesicle formation, movement, tethering and fusion. That Rab may be involved in polarized vesicular trafficking and neurotransmitter release. May participate in cell junction dynamics in Sertoli cells (By similarity).
Phosphorylation of Thr-72 in the switch II region by LRRK2 prevents the association of RAB regulatory proteins, including CHM, CHML and RAB GDP dissociation inhibitors GDI1 and GDI2.
Cell membrane>Lipid-anchor>Cytoplasmic side. Cytoplasmic vesicle>Phagosome. Cytoplasmic vesicle>Phagosome membrane>Lipid-anchor>Cytoplasmic side.
Note: Recruited to phagosomes containing S.aureus or M.tuberculosis.
Associated with actin, delta-catenin and alpha and beta tubulins (By similarity). Interacts with OTOF (By similarity). Interacts with PEX5R (By similarity). Interacts with RAB3IP. Interacts with VIM (By similarity). Interacts with CDH1 (By similarity). Interacts with MICALL2 (By similarity). Interacts with GDI1, GDI2, CHML and CHM; phosphorylation at Thr-72 disrupts these interactions.
Belongs to the small GTPase superfamily. Rab family.
研究领域
· Cellular Processes > Cellular community - eukaryotes > Tight junction. (View pathway)
限制条款
产品的规格、报价、验证数据请以官网为准，官网链接：www.affbiotech.com | www.affbiotech.cn（简体中文）| www.affbiotech.jp（日本語）产品的数据信息为Affinity所有，未经授权不得收集Affinity官网数据或资料用于商业用途，对抄袭产品数据的行为我们将保留诉诸法律的权利。
产品相关数据会因产品批次、产品检测情况随时调整，如您已订购该产品，请以订购时随货说明书为准，否则请以官网内容为准，官网内容有改动时恕不另行通知。
Affinity保证所销售产品均经过严格质量检测。如您购买的商品在规定时间内出现问题需要售后时，请您在Affinity官方渠道提交售后申请。产品仅供科学研究使用。不用于诊断和治疗。
产品未经授权不得转售。
Affinity Biosciences将不会对在使用我们的产品时可能发生的专利侵权或其他侵权行为负责。Affinity Biosciences, Affinity Biosciences标志和所有其他商标所有权归Affinity Biosciences LTD.