产品描述
*The optimal dilutions should be determined by the end user.
*Tips:
WB: 适用于变性蛋白样本的免疫印迹检测. IHC: 适用于组织样本的石蜡(IHC-p)或冰冻(IHC-f)切片样本的免疫组化/荧光检测. IF/ICC: 适用于细胞样本的荧光检测. ELISA(peptide): 适用于抗原肽的ELISA检测.
引用格式: Affinity Biosciences Cat# DF9839, RRID:AB_2843033.
展开/折叠
5730472O18Rik; dRalb; GTP binding protein; Ralb; RALB_HUMAN; RAS like protein B; Ras related GTP binding protein B; Ras-related protein Ral-B; v ral simian leukemia viral oncogene homolog B (ras related GTP binding protein); v ral simian leukemia viral oncogene homolog B (ras related); V ral simian leukemia viral oncogene homolog B;
抗原和靶标
- P11234 RALB_HUMAN:
- Protein BLAST With
- NCBI/
- ExPASy/
- Uniprot
MAANKSKGQSSLALHKVIMVGSGGVGKSALTLQFMYDEFVEDYEPTKADSYRKKVVLDGEEVQIDILDTAGQEDYAAIRDNYFRSGEGFLLVFSITEHESFTATAEFREQILRVKAEEDKIPLLVVGNKSDLEERRQVPVEEARSKAEEWGVQYVETSAKTRANVDKVFFDLMREIRTKKMSENKDKNGKKSSKNKKSFKERCCLL
种属预测
score>80的预测可信度较高，可尝试用于WB检测。*预测模型主要基于免疫原序列比对，结果仅作参考，不作为质保凭据。
High(score>80) Medium(80>score>50) Low(score<50) No confidence
翻译修饰 - P11234 作为底物
| Site | PTM Type | Enzyme | Source |
|---|---|---|---|
| S6 | Phosphorylation | Uniprot | |
| K7 | Ubiquitination | Uniprot | |
| S22 | Phosphorylation | Uniprot | |
| Y43 | Phosphorylation | Uniprot | |
| K47 | Ubiquitination | Uniprot | |
| Y51 | Phosphorylation | Uniprot | |
| Y75 | Phosphorylation | Uniprot | |
| Y82 | Phosphorylation | Uniprot | |
| S85 | Phosphorylation | Uniprot | |
| T104 | Phosphorylation | Uniprot | |
| K120 | Ubiquitination | Uniprot | |
| K129 | Ubiquitination | Uniprot | |
| K146 | Ubiquitination | Uniprot | |
| Y154 | Phosphorylation | Uniprot | |
| K160 | Ubiquitination | Uniprot | |
| K179 | Acetylation | Uniprot | |
| K180 | Acetylation | Uniprot | |
| K190 | Acetylation | Uniprot | |
| S198 | Phosphorylation | P17252 (PRKCA) | Uniprot |
研究背景
Multifunctional GTPase involved in a variety of cellular processes including gene expression, cell migration, cell proliferation, oncogenic transformation and membrane trafficking. Accomplishes its multiple functions by interacting with distinct downstream effectors. Acts as a GTP sensor for GTP-dependent exocytosis of dense core vesicles (By similarity). Required both to stabilize the assembly of the exocyst complex and to localize functional exocyst complexes to the leading edge of migrating cells (By similarity). Required for suppression of apoptosis. In late stages of cytokinesis, upon completion of the bridge formation between dividing cells, mediates exocyst recruitment to the midbody to drive abscission.
Prenylation is essential for membrane localization. The geranylgeranylated form and the farnesylated mutant does not undergo alternative prenylation in response to geranylgeranyltransferase I inhibitors (GGTIs) and farnesyltransferase I inhibitors (FTIs).
The farnesylated form confers resistance to the proapoptotic and anti-anchorage-dependent growth effects of geranylgeranyltransferase I inhibitors, including GGTI-2417.
Cell membrane>Lipid-anchor>Cytoplasmic side. Midbody.
Note: During late cytokinesis, enriched at the midbody.
Interacts with EXOC2/Sec5 and EXOC8/Exo84. Interacts (via effector domain) with RALBP1.
Belongs to the small GTPase superfamily. Ras family.
研究领域
· Environmental Information Processing > Signal transduction > Ras signaling pathway. (View pathway)
· Environmental Information Processing > Signal transduction > Rap1 signaling pathway. (View pathway)
· Environmental Information Processing > Signal transduction > Phospholipase D signaling pathway. (View pathway)
· Human Diseases > Cancers: Overview > Pathways in cancer. (View pathway)
· Human Diseases > Cancers: Specific types > Colorectal cancer. (View pathway)
· Human Diseases > Cancers: Specific types > Pancreatic cancer. (View pathway)
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