(2)
(3)
| 产品: | RHG24 抗体 |
| 货号: | DF9858 |
| 描述: | Rabbit polyclonal antibody to RHG24 |
| 应用: | WB IHC |
| 反应: | Human, Mouse, Rat |
| 预测: | Bovine, Horse, Sheep, Rabbit, Dog |
| 分子量: | 84 kDa; 84kD(Calculated). |
| 蛋白号: | Q8N264 |
| RRID: | AB_2843052 |
产品描述
*The optimal dilutions should be determined by the end user.
*Tips:
WB: 适用于变性蛋白样本的免疫印迹检测. IHC: 适用于组织样本的石蜡(IHC-p)或冰冻(IHC-f)切片样本的免疫组化/荧光检测. IF/ICC: 适用于细胞样本的荧光检测. ELISA(peptide): 适用于抗原肽的ELISA检测.
引用格式: Affinity Biosciences Cat# DF9858, RRID:AB_2843052.
展开/折叠
ARHGAP24; DKFZP564B1162; Filamin A associated RhoGAP; FILGAP; FLJ33877; p73; p73RhoGAP; RAC1 and CDC4 specific GTPase activating protein of 72kDa; RCGAP72; Rho GTPase activating protein 24; Rho type GTPase activating protein 24; RhoGAP of 73 kDa; Sarcoma antigen NY SAR 88;
抗原和靶标
Isoform 1 is widely expressed with a higher level in kidney. Isoform 2 is mainly expressed in endothelial cells.
- Q8N264 RHG24_HUMAN:
- Protein BLAST With
- NCBI/
- ExPASy/
- Uniprot
MEENNDSTENPQQGQGRQNAIKCGWLRKQGGFVKTWHTRWFVLKGDQLYYFKDEDETKPLGTIFLPGNKVSEHPCNEENPGKFLFEVVPGGDRDRMTANHESYLLMASTQNDMEDWVKSIRRVIWGPFGGGIFGQKLEDTVRYEKRYGNRLAPMLVEQCVDFIRQRGLKEEGLFRLPGQANLVKELQDAFDCGEKPSFDSNTDVHTVASLLKLYLRELPEPVIPYAKYEDFLSCAKLLSKEEEAGVKELAKQVKSLPVVNYNLLKYICRFLDEVQSYSGVNKMSVQNLATVFGPNILRPKVEDPLTIMEGTVVVQQLMSVMISKHDCLFPKDAELQSKPQDGVSNNNEIQKKATMGQLQNKENNNTKDSPSRQCSWDKSESPQRSSMNNGSPTALSGSKTNSPKNSVHKLDVSRSPPLMVKKNPAFNKGSGIVTNGSFSSSNAEGLEKTQTTPNGSLQARRSSSLKVSGTKMGTHSVQNGTVRMGILNSDTLGNPTNVRNMSWLPNGYVTLRDNKQKEQAGELGQHNRLSTYDNVHQQFSMMNLDDKQSIDSATWSTSSCEISLPENSNSCRSSTTTCPEQDFFGGNFEDPVLDGPPQDDLSHPRDYESKSDHRSVGGRSSRATSSSDNSETFVGNSSSNHSALHSLVSSLKQEMTKQKIEYESRIKSLEQRNLTLETEMMSLHDELDQERKKFTMIEIKMRNAERAKEDAEKRNDMLQKEMEQFFSTFGELTVEPRRTERGNTIWIQ
种属预测
score>80的预测可信度较高，可尝试用于WB检测。*预测模型主要基于免疫原序列比对，结果仅作参考，不作为质保凭据。
High(score>80) Medium(80>score>50) Low(score<50) No confidence
翻译修饰 - Q8N264 作为底物
| Site | PTM Type | Enzyme | Source |
|---|---|---|---|
| T62 | Phosphorylation | Uniprot | |
| T354 | Phosphorylation | Uniprot | |
| S369 | Phosphorylation | Uniprot | |
| S371 | Phosphorylation | Uniprot | |
| S385 | Phosphorylation | Uniprot | |
| S391 | Phosphorylation | Q13464 (ROCK1) | Uniprot |
| T393 | Phosphorylation | Uniprot | |
| S398 | Phosphorylation | Uniprot | |
| S402 | Phosphorylation | Q13464 (ROCK1) | Uniprot |
| S406 | Phosphorylation | Uniprot | |
| S413 | Phosphorylation | Q13464 (ROCK1) | Uniprot |
| S415 | Phosphorylation | Q13464 (ROCK1) | Uniprot |
| K421 | Acetylation | Uniprot | |
| K422 | Acetylation | Uniprot | |
| S437 | Phosphorylation | Q13464 (ROCK1) | Uniprot |
| T452 | Phosphorylation | Q13464 (ROCK1) | Uniprot |
| S464 | Phosphorylation | Uniprot | |
| Y508 | Phosphorylation | Uniprot | |
| S573 | Phosphorylation | Q13464 (ROCK1) | Uniprot |
| S574 | Phosphorylation | Q13464 (ROCK1) | Uniprot |
| T575 | Phosphorylation | Q13464 (ROCK1) | Uniprot |
| T576 | Phosphorylation | Q13464 (ROCK1) | Uniprot |
| T577 | Phosphorylation | Q13464 (ROCK1) | Uniprot |
| T624 | Phosphorylation | Uniprot | |
| S627 | Phosphorylation | Uniprot | |
| S727 | Phosphorylation | Uniprot | |
| T728 | Phosphorylation | Uniprot |
研究背景
Rho GTPase-activating protein involved in cell polarity, cell morphology and cytoskeletal organization. Acts as a GTPase activator for the Rac-type GTPase by converting it to an inactive GDP-bound state. Controls actin remodeling by inactivating Rac downstream of Rho leading to suppress leading edge protrusion and promotes cell retraction to achieve cellular polarity. Able to suppress RAC1 and CDC42 activity in vitro. Overexpression induces cell rounding with partial or complete disruption of actin stress fibers and formation of membrane ruffles, lamellipodia, and filopodia. Isoform 2 is a vascular cell-specific GAP involved in modulation of angiogenesis.
Phosphorylated by ROCK, leading to activate the RacGAP activity.
Cytoplasm>Cytoskeleton. Cell junction>Adherens junction. Cell junction>Focal adhesion. Cell projection.
Note: Localizes to actin stress fibers. In migrating cells, localizes to membrane lamellae and protusions.
Isoform 1 is widely expressed with a higher level in kidney. Isoform 2 is mainly expressed in endothelial cells.
Interacts with FLNA.
The coiled coil domain mediates the interaction with FLNA leading to its recruitment to lamellae.
文献引用
Application: WB Species: Pig Sample: neural cells
限制条款
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