产品描述
*The optimal dilutions should be determined by the end user.
*Tips:
WB: 适用于变性蛋白样本的免疫印迹检测. IHC: 适用于组织样本的石蜡(IHC-p)或冰冻(IHC-f)切片样本的免疫组化/荧光检测. IF/ICC: 适用于细胞样本的荧光检测. ELISA(peptide): 适用于抗原肽的ELISA检测.
引用格式: Affinity Biosciences Cat# DF9887, RRID:AB_2843081.
展开/折叠
OTTHUMP00000025825; Pim 2 oncogene; Pim 2h; Pim-2h; PIM2; PIM2 Oncogene; PIM2_HUMAN; Pim2h; Proto Oncogene PIM2; Proto-oncogene Pim-2 (serine threonine kinase); Serine/threonine kinase PIM2; Serine/threonine-protein kinase Pim-2;
抗原和靶标
Highly expressed in hematopoietic tissues, in leukemic and lymphoma cell lines, testis, small intestine, colon and colorectal adenocarcinoma cells. Weakly expressed in normal liver, but highly expressed in hepatocellular carcinoma tissues.
- Q9P1W9 PIM2_HUMAN:
- Protein BLAST With
- NCBI/
- ExPASy/
- Uniprot
MLTKPLQGPPAPPGTPTPPPGGKDREAFEAEYRLGPLLGKGGFGTVFAGHRLTDRLQVAIKVIPRNRVLGWSPLSDSVTCPLEVALLWKVGAGGGHPGVIRLLDWFETQEGFMLVLERPLPAQDLFDYITEKGPLGEGPSRCFFGQVVAAIQHCHSRGVVHRDIKDENILIDLRRGCAKLIDFGSGALLHDEPYTDFDGTRVYSPPEWISRHQYHALPATVWSLGILLYDMVCGDIPFERDQEILEAELHFPAHVSPDCCALIRRCLAPKPSSRPSLEEILLDPWMQTPAEDVPLNPSKGGPAPLAWSLLP
种属预测
score>80的预测可信度较高，可尝试用于WB检测。*预测模型主要基于免疫原序列比对，结果仅作参考，不作为质保凭据。
High(score>80) Medium(80>score>50) Low(score<50) No confidence
翻译修饰 - Q9P1W9 作为底物
| Site | PTM Type | Enzyme | Source |
|---|---|---|---|
| T3 | Phosphorylation | Uniprot | |
| T15 | Phosphorylation | Uniprot | |
| Y32 | Phosphorylation | Uniprot | |
| K40 | Ubiquitination | Uniprot | |
| K61 | Ubiquitination | Uniprot | |
| K89 | Ubiquitination | Uniprot | |
| K132 | Ubiquitination | Uniprot | |
| K165 | Ubiquitination | Uniprot | |
| K179 | Ubiquitination | Uniprot | |
| T195 | Phosphorylation | Q9P1W9 (PIM2) | Uniprot |
| S204 | Phosphorylation | Q9P1W9 (PIM2) | Uniprot |
翻译修饰 - Q9P1W9 作为激酶
| Substrate | Site | Source |
|---|---|---|
| P01106 (MYC) | S329 | Uniprot |
| P14618 (PKM) | T454 | Uniprot |
| P23588 (EIF4B) | S406 | Uniprot |
| P38936 (CDKN1A) | T145 | Uniprot |
| P38936 (CDKN1A) | S146 | Uniprot |
| P41279 (MAP3K8) | S400 | Uniprot |
| P41279 (MAP3K8) | S413 | Uniprot |
| P46527 (CDKN1B) | T157 | Uniprot |
| P46527 (CDKN1B) | T198 | Uniprot |
| P49815 (TSC2) | S1798 | Uniprot |
| Q00987 (MDM2) | S166 | Uniprot |
| Q00987 (MDM2) | S186 | Uniprot |
| Q13541 (EIF4EBP1) | S65 | Uniprot |
| Q92934 (BAD) | S75 | Uniprot |
| Q92934 (BAD) | S99 | Uniprot |
| Q92934 (BAD) | S118 | Uniprot |
| Q9P1W9 (PIM2) | T195 | Uniprot |
| Q9P1W9 (PIM2) | S204 | Uniprot |
研究背景
Proto-oncogene with serine/threonine kinase activity involved in cell survival and cell proliferation. Exerts its oncogenic activity through: the regulation of MYC transcriptional activity, the regulation of cell cycle progression, the regulation of cap-dependent protein translation and through survival signaling by phosphorylation of a pro-apoptotic protein, BAD. Phosphorylation of MYC leads to an increase of MYC protein stability and thereby an increase transcriptional activity. The stabilization of MYC exerted by PIM2 might explain partly the strong synergism between these 2 oncogenes in tumorigenesis. Regulates cap-dependent protein translation in a mammalian target of rapamycin complex 1 (mTORC1)-independent manner and in parallel to the PI3K-Akt pathway. Mediates survival signaling through phosphorylation of BAD, which induces release of the anti-apoptotic protein Bcl-X(L)/BCL2L1. Promotes cell survival in response to a variety of proliferative signals via positive regulation of the I-kappa-B kinase/NF-kappa-B cascade; this process requires phosphorylation of MAP3K8/COT. Promotes growth factor-independent proliferation by phosphorylation of cell cycle factors such as CDKN1A and CDKN1B. Involved in the positive regulation of chondrocyte survival and autophagy in the epiphyseal growth plate.
Autophosphorylated.
Highly expressed in hematopoietic tissues, in leukemic and lymphoma cell lines, testis, small intestine, colon and colorectal adenocarcinoma cells. Weakly expressed in normal liver, but highly expressed in hepatocellular carcinoma tissues.
Interacts with MYC.
Belongs to the protein kinase superfamily. CAMK Ser/Thr protein kinase family. PIM subfamily.
研究领域
· Human Diseases > Cancers: Overview > Pathways in cancer. (View pathway)
· Human Diseases > Cancers: Specific types > Acute myeloid leukemia. (View pathway)
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