| 产品: | RIOK1 抗体 |
| 货号: | DF9888 |
| 描述: | Rabbit polyclonal antibody to RIOK1 |
| 应用: | WB IHC |
| 反应: | Human, Mouse, Rat |
| 预测: | Pig, Zebrafish, Bovine, Horse, Sheep, Dog, Xenopus |
| 分子量: | 66 kDa; 66kD(Calculated). |
| 蛋白号: | Q9BRS2 |
| RRID: | AB_2843082 |
产品描述
*The optimal dilutions should be determined by the end user.
*Tips:
WB: 适用于变性蛋白样本的免疫印迹检测. IHC: 适用于组织样本的石蜡(IHC-p)或冰冻(IHC-f)切片样本的免疫组化/荧光检测. IF/ICC: 适用于细胞样本的荧光检测. ELISA(peptide): 适用于抗原肽的ELISA检测.
引用格式: Affinity Biosciences Cat# DF9888, RRID:AB_2843082.
展开/折叠
AD034; bA288G3.1; RIO kinase 1 (yeast); RIO kinase 1; RIOK1; RIOK1_HUMAN; Serine/threonine-protein kinase rio1;
抗原和靶标
- Q9BRS2 RIOK1_HUMAN:
- Protein BLAST With
- NCBI/
- ExPASy/
- Uniprot
MDYRRLLMSRVVPGQFDDADSSDSENRDLKTVKEKDDILFEDLQDNVNENGEGEIEDEEEEGYDDDDDDWDWDEGVGKLAKGYVWNGGSNPQANRQTSDSSSAKMSTPADKVLRKFENKINLDKLNVTDSVINKVTEKSRQKEADMYRIKDKADRATVEQVLDPRTRMILFKMLTRGIITEINGCISTGKEANVYHASTANGESRAIKIYKTSILVFKDRDKYVSGEFRFRHGYCKGNPRKMVKTWAEKEMRNLIRLNTAEIPCPEPIMLRSHVLVMSFIGKDDMPAPLLKNVQLSESKARELYLQVIQYMRRMYQDARLVHADLSEFNMLYHGGGVYIIDVSQSVEHDHPHALEFLRKDCANVNDFFMRHSVAVMTVRELFEFVTDPSITHENMDAYLSKAMEIASQRTKEERSSQDHVDEEVFKRAYIPRTLNEVKNYERDMDIIMKLKEEDMAMNAQQDNILYQTVTGLKKDLSGVQKVPALLENQVEERTCSDSEDIGSSECSDTDSEEQGDHARPKKHTTDPDIDKKERKKMVKEAQREKRKNKIPKHVKKRKEKTAKTKKGK
种属预测
score>80的预测可信度较高，可尝试用于WB检测。*预测模型主要基于免疫原序列比对，结果仅作参考，不作为质保凭据。
High(score>80) Medium(80>score>50) Low(score<50) No confidence
翻译修饰 - Q9BRS2 作为底物
| Site | PTM Type | Enzyme | Source |
|---|---|---|---|
| S21 | Phosphorylation | Uniprot | |
| S22 | Phosphorylation | Uniprot | |
| S24 | Phosphorylation | Uniprot | |
| K81 | Ubiquitination | Uniprot | |
| Y83 | Phosphorylation | Uniprot | |
| S89 | Phosphorylation | Uniprot | |
| R95 | Methylation | Uniprot | |
| S102 | Phosphorylation | Uniprot | |
| T107 | Phosphorylation | Uniprot | |
| K124 | Ubiquitination | Uniprot | |
| T175 | Phosphorylation | Uniprot | |
| S204 | Phosphorylation | Uniprot | |
| K218 | Ubiquitination | Uniprot | |
| Y223 | Phosphorylation | Uniprot | |
| S225 | Phosphorylation | Uniprot | |
| K299 | Ubiquitination | Uniprot | |
| T410 | Phosphorylation | Uniprot | |
| K411 | Methylation | Uniprot | |
| K426 | Methylation | Uniprot | |
| K426 | Ubiquitination | Uniprot | |
| K438 | Ubiquitination | Uniprot | |
| Y440 | Phosphorylation | Uniprot | |
| K451 | Ubiquitination | Uniprot | |
| Y466 | Phosphorylation | Uniprot | |
| T468 | Phosphorylation | Uniprot | |
| T470 | Phosphorylation | Uniprot | |
| K473 | Ubiquitination | Uniprot | |
| K474 | Ubiquitination | Uniprot | |
| K481 | Ubiquitination | Uniprot | |
| T494 | Phosphorylation | Uniprot | |
| T509 | Phosphorylation | Uniprot | |
| K539 | Ubiquitination | Uniprot |
研究背景
Involved in the final steps of cytoplasmic maturation of the 40S ribosomal subunit. Involved in processing of 18S-E pre-rRNA to the mature 18S rRNA. Required for the recycling of NOB1 and PNO1 from the late 40S precursor. The association with the very late 40S subunit intermediate may involve a translation-like checkpoint point cycle preceeding the binding to the 60S ribosomal subunit (By similarity). Despite the protein kinase domain is proposed to act predominantly as an ATPase (By similarity). The catalytic activity regulates its dynamic association with the 40S subunit (By similarity). In addition to its role in ribosomal biogenesis acts as an adapter protein by recruiting NCL/nucleolin the to PRMT5 complex for its symmetrical methylation.
Cytoplasm>Cytosol.
Associates with the precursor of the 40S ribosome subunit. Interacts (via its N-terminus) with PRMT5 (via its N-terminus). Interacts with WDR77. Found in a PRMT5 complex composed of PRMT5, WDR77 and RIOK1. Interacts (via its C-terminus) with NCL; this interaction targets NCL for PRTM5 methylation.
Belongs to the protein kinase superfamily. RIO-type Ser/Thr kinase family.
研究领域
· Genetic Information Processing > Translation > Ribosome biogenesis in eukaryotes.
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