产品描述
*The optimal dilutions should be determined by the end user.
*Tips:
WB: 适用于变性蛋白样本的免疫印迹检测. IHC: 适用于组织样本的石蜡(IHC-p)或冰冻(IHC-f)切片样本的免疫组化/荧光检测. IF/ICC: 适用于细胞样本的荧光检测. ELISA(peptide): 适用于抗原肽的ELISA检测.
引用格式: Affinity Biosciences Cat# DF9899, RRID:AB_2843093.
展开/折叠
54 kDa VacA-interacting protein; 54 kDa vimentin-interacting protein; 90 kDa SH3 protein interacting with Nck; AF3p21; dia interacting protein; Dia-interacting protein 1; Diaphanous protein-interacting protein; DIP; DIP-1; DIP1; NCK interacting protein with SH3 domain; NCK-interacting protein with SH3 domain; Nckipsd; ORF1; SH3 adapter protein SPIN90; SH3 protein interacting with Nck 90 kDa; SPIN90; SPN90_HUMAN; VIP54; WASLBP; WASP-interacting SH3-domain protein; WISH; wiskott- Aldrich syndrome protein-interacting protein; Wiskott-Aldrich syndrome protein-interacting protein;
抗原和靶标
Highest expression in heart, brain, skeletal muscle, kidney and liver. Lower levels in placenta, lung, small intestine and leukocytes. Weak expression in colon, thymus and spleen.
- Q9NZQ3 SPN90_HUMAN:
- Protein BLAST With
- NCBI/
- ExPASy/
- Uniprot
MYRALYAFRSAEPNALAFAAGETFLVLERSSAHWWLAARARSGETGYVPPAYLRRLQGLEQDVLQAIDRAIEAVHNTAMRDGGKYSLEQRGVLQKLIHHRKETLSRRGPSASSVAVMTSSTSDHHLDAAAARQPNGVCRAGFERQHSLPSSEHLGADGGLYQIPLPSSQIPPQPRRAAPTTPPPPVKRRDREALMASGSGGHNTMPSGGNSVSSGSSVSSTSLDTLYTSSSPSEPGSSCSPTPPPVPRRGTHTTVSQVQPPPSKASAPEPPAEEEVATGTTSASDDLEALGTLSLGTTEEKAAAEAAVPRTIGAELMELVRRNTGLSHELCRVAIGIIVGHIQASVPASSPVMEQVLLSLVEGKDLSMALPSGQVCHDQQRLEVIFADLARRKDDAQQRSWALYEDEGVIRCYLEELLHILTDADPEVCKKMCKRNEFESVLALVAYYQMEHRASLRLLLLKCFGAMCSLDAAIISTLVSSVLPVELARDMQTDTQDHQKLCYSALILAMVFSMGEAVPYAHYEHLGTPFAQFLLNIVEDGLPLDTTEQLPDLCVNLLLALNLHLPAADQNVIMAALSKHANVKIFSEKLLLLLNRGDDPVRIFKHEPQPPHSVLKFLQDVFGSPATAAIFYHTDMMALIDITVRHIADLSPGDKLRMEYLSLMHAIVRTTPYLQHRHRLPDLQAILRRILNEEETSPQCQMDRMIVREMCKEFLVLGEAPS
种属预测
score>80的预测可信度较高，可尝试用于WB检测。*预测模型主要基于免疫原序列比对，结果仅作参考，不作为质保凭据。
High(score>80) Medium(80>score>50) Low(score<50) No confidence
翻译修饰 - Q9NZQ3 作为底物
| Site | PTM Type | Enzyme | Source |
|---|---|---|---|
| K84 | Ubiquitination | Uniprot | |
| Y85 | Phosphorylation | Uniprot | |
| K95 | Ubiquitination | Uniprot | |
| R107 | Methylation | Uniprot | |
| S110 | Phosphorylation | Uniprot | |
| S113 | Phosphorylation | Uniprot | |
| S120 | Phosphorylation | Uniprot | |
| S122 | Phosphorylation | Uniprot | |
| S147 | Phosphorylation | Uniprot | |
| Y161 | Phosphorylation | P12931 (SRC) | Uniprot |
| T180 | Phosphorylation | Uniprot | |
| T181 | Phosphorylation | Uniprot | |
| Y227 | Phosphorylation | P12931 (SRC) | Uniprot |
| R249 | Methylation | Uniprot | |
| T251 | Phosphorylation | Uniprot | |
| T253 | Phosphorylation | Uniprot | |
| T254 | Phosphorylation | Uniprot | |
| S256 | Phosphorylation | Uniprot | |
| S263 | Phosphorylation | Uniprot | |
| T311 | Phosphorylation | Uniprot | |
| Y404 | Phosphorylation | Uniprot | |
| K584 | Ubiquitination | Uniprot | |
| Y673 | Phosphorylation | Uniprot | |
| K712 | Ubiquitination | Uniprot |
研究背景
Has an important role in stress fiber formation induced by active diaphanous protein homolog 1 (DRF1). Induces microspike formation, in vivo (By similarity). In vitro, stimulates N-WASP-induced ARP2/3 complex activation in the absence of CDC42 (By similarity). May play an important role in the maintenance of sarcomeres and/or in the assembly of myofibrils into sarcomeres. Implicated in regulation of actin polymerization and cell adhesion. Plays a role in angiogenesis.
Nucleus.
Note: Colocalizes with DRF1 at membrane ruffles, and with Nck at Z-disks in mature cardiac myocytes.
Highest expression in heart, brain, skeletal muscle, kidney and liver. Lower levels in placenta, lung, small intestine and leukocytes. Weak expression in colon, thymus and spleen.
Associates with the intermediate filaments, vimentin and desmin. Binds the first and third SH3 domains of NCK. Binds the proline-rich domains of N-WASP through its SH3 domain (By similarity). Similarly, binds diaphanous protein homolog 1 (DRF1). Binds the SH3 domains of GRB2 through its proline-rich domains. Interacts with Helicobacter pylori toxin vacA. Isoform 4 interacts with FHOD1. Interacts with FASLG. Interacts with TMIGD2.
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