SLIT3 Antibody - #DF9909

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产品: SLIT3 抗体
货号: DF9909
描述: Rabbit polyclonal antibody to SLIT3
应用: WB IHC IF/ICC
反应: Human, Mouse, Rat
预测: Pig, Bovine, Horse, Sheep, Rabbit, Dog, Chicken
分子量: 168 kDa; 168kD(Calculated).
蛋白号: O75094
RRID: AB_2843103

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   规格 价格 库存
 50ul RMB¥ 1500 现货
 100ul RMB¥ 2300 现货
 200ul RMB¥ 3000 现货

货期: 当天发货

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MS Report
HPLC Report
MSDS
说明书
COA
实验方案
WB Handbook
IHC Protocol
IF/ICC Protocol

产品描述

来源:
Rabbit
应用:
WB 1:1000-3000, IF/ICC 1:100-1:500, IHC 1:50-1:200
*The optimal dilutions should be determined by the end user.
*Tips:

WB: 适用于变性蛋白样本的免疫印迹检测. IHC: 适用于组织样本的石蜡(IHC-p)或冰冻(IHC-f)切片样本的免疫组化/荧光检测. IF/ICC: 适用于细胞样本的荧光检测. ELISA(peptide): 适用于抗原肽的ELISA检测.

反应:
Human,Mouse,Rat
预测:
Pig(100%), Bovine(100%), Horse(100%), Sheep(100%), Rabbit(100%), Dog(100%), Chicken(92%)
克隆:
Polyclonal
特异性:
SLIT3 Antibody detects endogenous levels of total SLIT3.
RRID:
AB_2843103
引用格式: Affinity Biosciences Cat# DF9909, RRID:AB_2843103.
偶联:
Unconjugated.
纯化:
The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).
保存:
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
别名:

展开/折叠

FLJ10764; KIAA0814; MEGF 5; MEGF5; Multiple EGF-like domains protein 5; Multiple epidermal growth factor like domains 5; Multiple epidermal growth factor-like domains protein 5; SLIL 2; SLIL2; SLIT 1; Slit 2; Slit 3; Slit homolog 3 (Drosophila); Slit homolog 3; Slit homolog 3 protein; Slit-3; SLIT1; Slit2; Slit3; SLIT3_HUMAN;

抗原和靶标

免疫原:
Uniprot:

O75094(SLIT3_HUMAN) >>Visit HPA database.

基因/基因ID:
SLIT3(6586)
表达:
O75094 SLIT3_HUMAN:

Predominantly expressed in thyroid.

序列:
MAPGWAGVGAAVRARLALALALASVLSGPPAVACPTKCTCSAASVDCHGLGLRAVPRGIPRNAERLDLDRNNITRITKMDFAGLKNLRVLHLEDNQVSVIERGAFQDLKQLERLRLNKNKLQVLPELLFQSTPKLTRLDLSENQIQGIPRKAFRGITDVKNLQLDNNHISCIEDGAFRALRDLEILTLNNNNISRILVTSFNHMPKIRTLRLHSNHLYCDCHLAWLSDWLRQRRTVGQFTLCMAPVHLRGFNVADVQKKEYVCPAPHSEPPSCNANSISCPSPCTCSNNIVDCRGKGLMEIPANLPEGIVEIRLEQNSIKAIPAGAFTQYKKLKRIDISKNQISDIAPDAFQGLKSLTSLVLYGNKITEIVKGLFDGLVSLQLLLLNANKINCLRVNTFQDLQNLNLLSLYDNKLQTISKGLFAPLQSIQTLHLAQNPFVCDCHLKWLADYLQDNPIETSGARCSSPRRLANKRISQIKSKKFRCSGSEDYRSRFSSECFMDLVCPEKCRCEGTIVDCSNQKLVRIPSHLPEYVTDLRLNDNEVSVLEATGIFKKLPNLRKINLSNNKIKEVREGAFDGAASVQELMLTGNQLETVHGRVFRGLSGLKTLMLRSNLIGCVSNDTFAGLSSVRLLSLYDNRITTITPGAFTTLVSLSTINLLSNPFNCNCHLAWLGKWLRKRRIVSGNPRCQKPFFLKEIPIQDVAIQDFTCDGNEESSCQLSPRCPEQCTCMETVVRCSNKGLRALPRGMPKDVTELYLEGNHLTAVPRELSALRHLTLIDLSNNSISMLTNYTFSNMSHLSTLILSYNRLRCIPVHAFNGLRSLRVLTLHGNDISSVPEGSFNDLTSLSHLALGTNPLHCDCSLRWLSEWVKAGYKEPGIARCSSPEPMADRLLLTTPTHRFQCKGPVDINIVAKCNACLSSPCKNNGTCTQDPVELYRCACPYSYKGKDCTVPINTCIQNPCQHGGTCHLSDSHKDGFSCSCPLGFEGQRCEINPDDCEDNDCENNATCVDGINNYVCICPPNYTGELCDEVIDHCVPELNLCQHEAKCIPLDKGFSCECVPGYSGKLCETDNDDCVAHKCRHGAQCVDTINGYTCTCPQGFSGPFCEHPPPMVLLQTSPCDQYECQNGAQCIVVQQEPTCRCPPGFAGPRCEKLITVNFVGKDSYVELASAKVRPQANISLQVATDKDNGILLYKGDNDPLALELYQGHVRLVYDSLSSPPTTVYSVETVNDGQFHSVELVTLNQTLNLVVDKGTPKSLGKLQKQPAVGINSPLYLGGIPTSTGLSALRQGTDRPLGGFHGCIHEVRINNELQDFKALPPQSLGVSPGCKSCTVCKHGLCRSVEKDSVVCECRPGWTGPLCDQEARDPCLGHRCHHGKCVATGTSYMCKCAEGYGGDLCDNKNDSANACSAFKCHHGQCHISDQGEPYCLCQPGFSGEHCQQENPCLGQVVREVIRRQKGYASCATASKVPIMECRGGCGPQCCQPTRSKRRKYVFQCTDGSSFVEEVERHLECGCLACS

种属预测

种属预测:

score>80的预测可信度较高，可尝试用于WB检测。*预测模型主要基于免疫原序列比对，结果仅作参考，不作为质保凭据。

Species
Results
Score
Pig
100
Horse
100
Bovine
100
Sheep
100
Dog
100
Rabbit
100
Chicken
92
Xenopus
0
Zebrafish
0
Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

翻译修饰 - O75094 作为底物

Site PTM Type Enzyme
T417 Phosphorylation
S419 Phosphorylation
K473 Acetylation
K561 Acetylation
S582 Phosphorylation
S605 Phosphorylation
T609 Phosphorylation
S1173 Phosphorylation
T1295 O-Glycosylation
T1385 Phosphorylation
T1387 Phosphorylation
S1388 Phosphorylation
Y1389 Phosphorylation

研究背景

功能:

May act as molecular guidance cue in cellular migration, and function may be mediated by interaction with roundabout homolog receptors.

细胞定位:

Secreted.

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionSubcellular location
组织特异性:

Predominantly expressed in thyroid.

研究领域

· Organismal Systems > Development > Axon guidance.   (View pathway)

文献引用

1). A novel epithelial-mesenchymal transition (EMT)-related gene signature of predictive value for the survival outcomes in lung adenocarcinoma. Frontiers in Oncology, 2022 (PubMed: 36185284) [IF=4.7]

Application: WB    Species: Human    Sample: LUAD tumor

Figure 2 The identification 4 prognostic EMT-related genes comprising the E-signature. (A) The standard deviation distribution of all mRNAs. In the left panel, location corresponding to the red columns were standard deviation (SD) of genes with frequency greater than 500, where SD was used as the abscissa and the vertical axis represented the number of genes. The right panel showed that the gene frequency distribution chart obtained by LASSO analysis. The green baseline meant that frequency was equal to 500, the part exceeded the green line was genes with frequency greater than 500. (B) KM curves showed the overall survival (OS) of patients grouped according to expression patterns of 4 prognostic EMT-related genes comprising the E-signature in TCGA-LUAD datasets. (C) Differential expression analysis of DKK1, LOXL2, MGP and SLIT3 originated from TCGA-LUAD dataset. (D) The expression of 4 genes in fresh LUAD tumor samples (T) and adjacent normal-frozen tissues (N) detected by Western blot
2). Targeting Bioinformatics Predicted Biomarkers Associated with Cell Proliferation and Migration for Treating Gliomas: Preclinical Studies in a GL261 Mouse Model. Neuroglia, 2023

Application: IHC    Species: Human    Sample:

Figure 1. Expressions of PLXNB2, SLIT3, and SPON1 in human high-grade gliomas. Representative immunohistochemistry (IHC) images for PLXNB2 (A), SLIT3 (B), and SPON1 (C) in human GBM tumor sections. (D) Negative control in a low-grade glioma for SLIT3. Magnification is 20× for slides in (A–D). (E) Percentage (%) of patient protein expression (IHC) for PLXNB2, SLIT3, and SPON1 in high-grade (HG) gliomas highly expressed (HG E) or not expressed (HG NE). The % of patient expression for each biomarker in HG was 80% or higher.

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