CXCL5 Antibody - #DF9919

>>买2送1 进行中>>
>>赠JD卡 进行中>>
(9)   (12)  
产品: CXCL5 抗体
货号: DF9919
描述: Rabbit polyclonal antibody to CXCL5
应用: WB IHC
文献验证: WB, IHC
反应: Human, Mouse, Rat
预测: Rabbit
分子量: 12 kDa; 12kD(Calculated).
蛋白号: P42830
RRID: AB_2843113

浏览相似产品>>

   规格 价格 库存
 50ul RMB¥ 1250 现货
 100ul RMB¥ 2300 现货
 200ul RMB¥ 3000 现货

货期: 当天发货

联系销售
相关下载
MS Report
HPLC Report
MSDS
说明书
COA
实验方案
WB Handbook
IHC Protocol
IF/ICC Protocol

产品描述

来源:
Rabbit
应用:
WB 1:1000-3000, IHC 1:50-1:200
*The optimal dilutions should be determined by the end user.
*Tips:

WB: 适用于变性蛋白样本的免疫印迹检测. IHC: 适用于组织样本的石蜡(IHC-p)或冰冻(IHC-f)切片样本的免疫组化/荧光检测. IF/ICC: 适用于细胞样本的荧光检测. ELISA(peptide): 适用于抗原肽的ELISA检测.

反应:
Human, Mouse, Rat
预测:
Rabbit(83%)
克隆:
Polyclonal
特异性:
CXCL5 Antibody detects endogenous levels of total CXCL5.
RRID:
AB_2843113
引用格式: Affinity Biosciences Cat# DF9919, RRID:AB_2843113.
偶联:
Unconjugated.
纯化:
The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).
保存:
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
别名:

展开/折叠

AMCFII; C-X-C motif chemokine 5; C-X-C motif chemokine ligand 5; chemokine (C-X-C motif) ligand 5; Cxcl5; CXCL5_HUMAN; ENA 78; ENA-78 (8-78); ENA-78(1-78); ENA-78(9-78); ENA78; Epithelial derived neutrophil activating protein 78; Epithelial-derived neutrophil-activating protein 78; Lipopolysaccharide-induced CXC chemokine; Neutrophil activating peptide ENA 78; Neutrophil-activating peptide ENA-78; neutrophil-activating protein 78; SCYB5; Small inducible cytokine B5; small inducible cytokine subfamily B (Cys-X-Cys), member 5 (epithelial-derived neutrophil-activating peptide 78); small inducible cytokine subfamily B, member 5; Small-inducible cytokine B5;

抗原和靶标

免疫原:
Uniprot:

P42830(CXCL5_HUMAN) >>Visit HPA database.

基因/基因ID:
CXCL5(6374)
序列:
MSLLSSRAARVPGPSSSLCALLVLLLLLTQPGPIASAGPAAAVLRELRCVCLQTTQGVHPKMISNLQVFAIGPQCSKVEVVASLKNGKEICLDPEAPFLKKVIQKILDGGNKEN

种属预测

种属预测:

score>80的预测可信度较高，可尝试用于WB检测。*预测模型主要基于免疫原序列比对，结果仅作参考，不作为质保凭据。

Species
Results
Score
Rabbit
83
Pig
0
Horse
0
Bovine
0
Sheep
0
Dog
0
Xenopus
0
Zebrafish
0
Chicken
0
Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

研究背景

功能:

Involved in neutrophil activation. In vitro, ENA-78(8-78) and ENA-78(9-78) show a threefold higher chemotactic activity for neutrophil granulocytes.

翻译修饰:

N-terminal processed forms ENA-78(8-78) and ENA-78(9-78) are produced by proteolytic cleavage after secretion from peripheral blood monocytes.

细胞定位:

Secreted.

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionSubcellular location
亚基结构:

Monomer. Homodimer.

蛋白家族:

Belongs to the intercrine alpha (chemokine CxC) family.

研究领域

· Environmental Information Processing > Signaling molecules and interaction > Cytokine-cytokine receptor interaction.   (View pathway)

· Environmental Information Processing > Signal transduction > TNF signaling pathway.   (View pathway)

· Human Diseases > Infectious diseases: Bacterial > Pertussis.

· Human Diseases > Immune diseases > Rheumatoid arthritis.

· Organismal Systems > Immune system > Chemokine signaling pathway.   (View pathway)

· Organismal Systems > Immune system > IL-17 signaling pathway.   (View pathway)

文献引用

1). Taraxasterol regulates p53 transcriptional activity to inhibit pancreatic cancer by inducing MDM2 ubiquitination degradation. Phytomedicine : international journal of phytotherapy and phytopharmacology, 2024 (PubMed: 39671783) [IF=7.9]
2). Concomitant NAFLD Facilitates Liver Metastases and PD-1-Refractory by Recruiting MDSCs via CXCL5/CXCR2 in Colorectal Cancer. Cellular and molecular gastroenterology and hepatology, 2024 (PubMed: 38724007) [IF=7.1]

Application: IF/ICC    Species: Mouse    Sample:

Figure 6. NAFLD promotes CXCL5 and recruits CXCR2+ MDSCs accumulation in liver metastases. (A) Mouse XL Cytokine Array detects multiple cytokines, chemokines, growth factors, and other soluble proteins in serum. Data shown are from a 5-minute exposure to Bio-Rad, top: CRLM group, bottom: NAFLD with CRLM group. (B) Relative cytokine levels in serum of each tumor-bearing C57BL/6 mice models. (C) Localization of CXCL5 and CXCR2 reactivity in tissue samples of ob/ob and WT group, acquired by confocal imaging. Scale bars represent 100 μm, upper panel; 20 μm, lower panel.
3). CXCL5 Downregulation in Villous Tissue Is Correlated With Recurrent Spontaneous Abortion. Frontiers in Immunology, 2021 (PubMed: 34603292) [IF=5.7]

Application: IHC    Species: Human    Sample: placental villous tissue

Figure 1 CXCL5 expression in human placental villous tissue from the RSA group and the control group. (A) Expression of CXCL5 mRNA in the RSA group (n = 15) and control group (n = 13) by RT-PCR. (B, C) Representative immunohistochemical staining images and quantification of CXCL5 in the RSA group (n = 15) and normal group (n = 13). Relative gene expression was normalized to GAPDH. ImageJ is used to quantify the staining intensity. Magnification, ×200 and ×400. Scale bars = 100 μm. Data are presented as the mean ± SD. Results are reported as fold change compared with the control group. (*p < 0.05, **p < 0.01). RSA, recurrent spontaneous abortion; mRNA, messenger RNA; RT‐PCR, quantitative real-time polymerase chain reaction; CXCL5, C-X-C motif chemokine ligand 5; IHC, immunohistochemistry.

Application: IHC    Species: Human    Sample: placental villous tissue

Figure 1 CXCL5 expression in human placental villous tissue from the RSA group and the control group. (A) Expression of CXCL5 mRNA in the RSA group (n = 15) and control group (n = 13) by RT-PCR. (B, C) Representative immunohistochemical staining images and quantification of CXCL5 in the RSA group (n = 15) and normal group (n = 13). Relative gene expression was normalized to GAPDH. ImageJ is used to quantify the staining intensity. Magnification, ×200 and ×400. Scale bars = 100 μm. Data are presented as the mean ± SD. Results are reported as fold change compared with the control group. (*p < 0.05, **p < 0.01). RSA, recurrent spontaneous abortion; mRNA, messenger RNA; RT‐PCR, quantitative real-time polymerase chain reaction; CXCL5, C-X-C motif chemokine ligand 5; IHC, immunohistochemistry.
4). Transcriptomics Reveals Effect of Pulsatilla Decoction Butanol Extract in Alleviating Vulvovaginal Candidiasis by Inhibiting Neutrophil Chemotaxis and Activation via TLR4 Signaling. Pharmaceuticals (Basel, Switzerland), 2024 (PubMed: 38794163) [IF=4.6]

Application: WB    Species: Mouse    Sample:

Figure 13. BEPD downregulated the expression of chemokine-associated proteins (Western blots). Western blots were utilized to detect the expression of CXCL1, CXCL3, S100A8, and S100A9 proteins. Values are presented as mean ± SD. n = 3. ** p < 0.01, vs. control group; *** p < 0.01, vs. control group; # p < 0.05, vs. model group; ## p < 0.01, vs. model group; ### p < 0.001, vs. model group. BEPD, n-butanol extract of Pulsatilla decoction; BEPD-L, low-dose BEPD group (20 mL/kg); BEPD-M, medium-dose BEPD group (40 mL/kg); BEPD-H, high-dose BEPD group (80 mL/kg); Flu, fluconazole group.
5). MiR-597-5p inhibits carcinogenesis and macrophage recruitment in colitis-related colorectal cancer via reducing the expression of CXCL5. Cancer biology & therapy, 2023 (PubMed: 37942533) [IF=4.4]

Application: IHC    Species: Mouse    Sample:

Figure 4. CXCL5 antibody attenuates mucosal injury and slows down the development of neoplasia in AOM/DSS-treated miR-597-5pIEC/- mice. (a) the colon length ofmice was gauged. N = 3. (b) the number of tumors was calculated after 18 weeks of AOM/DSS exposure. N = 6. (c-d) histological colitis scores of mice andrepresentative images of histological sections in each group. N = 3. *P < .05, **P < .01, ***P < .001.6 S. LI ET AL.
6). Integrative analysis reveals chemokines CCL2 and CXCL5 mediated shear stress-induced aortic dissection formation. Heliyon, 2023 (PubMed: 38163105) [IF=4.0]

Application: WB    Species: human    Sample:

Fig. 6 Chemokine receptor and ligand profiles in pulsatile shear stress-induced HAEC and AD. (A–C) Trends of chemokine receptors and ligands change over time in shear stress-induced HAEC. (D–F) Significantly differently expressed chemokine receptors and ligands in three AD datasets. (G, H) Immunoblotting validation of chemokines CCL2 and CXCL5 is significantly differently expressed in the three AD datasets. (I, J) Quantitative data of relative protein level to β-actin. At least three experiments were repeated, and the representative figures are shown. *P < 0.05, ***P < 0.001 compared with the control group. Before statistical comparisons, normality and equal variance were first tested. After checking for similar variance among normally distributed data, differences between the two groups were analyzed by Student's t-test.
7). TSZAF monomer combination downregulates the Wnt/β-catenin signaling pathway and inhibits neutrophil recruitment to prevent lung cancer metastasis. Chinese Journal of Natural Medicines, 2025 [IF=4.0]

Application: WB    Species: Mouse    Sample: lung cancer cells

Figure 7. TSZAF mc decreased the secretion of CXCL5 from tumor cells to reduce neutrophils infiltration. (A) CTC-TJH-01 cells was treated with TSZAF mc (0, 0.5, 1 μM) for 24 h, the relative mRNA expression levels of CXCL1, CXCL5 and IL-8 was analyzed by RT-PCR. (B) The CTC-TJH-01 and LLC cells were exposed to TSZAF mc (0, 0.5, 1 μM) for 24 h, the protein expression of CXCL1, CXCL5 and IL-8 were detected by WB. β-actin was used as an internal standard. (C) The proportion of neutrophils recruited by LLC cells in vitro was detected by flow cytometry. (D) The IF staining results of Ly6G expression in lung metastases. Scale bar 50 μm. (E) The immunohistochemistry staining results of CXCL5 expression in lung metastases. Scale bar 40 μm. n=8, *P < 0.05, **P < 0.01, ***P < 0.001 compared with the control group.

Application: IHC    Species: Mouse    Sample: lung cancer cells

Figure 7. TSZAF mc decreased the secretion of CXCL5 from tumor cells to reduce neutrophils infiltration. (A) CTC-TJH-01 cells was treated with TSZAF mc (0, 0.5, 1 μM) for 24 h, the relative mRNA expression levels of CXCL1, CXCL5 and IL-8 was analyzed by RT-PCR. (B) The CTC-TJH-01 and LLC cells were exposed to TSZAF mc (0, 0.5, 1 μM) for 24 h, the protein expression of CXCL1, CXCL5 and IL-8 were detected by WB. β-actin was used as an internal standard. (C) The proportion of neutrophils recruited by LLC cells in vitro was detected by flow cytometry. (D) The IF staining results of Ly6G expression in lung metastases. Scale bar 50 μm. (E) The immunohistochemistry staining results of CXCL5 expression in lung metastases. Scale bar 40 μm. n=8, *P < 0.05, **P < 0.01, ***P < 0.001 compared with the control group.
8). LncRNA NEAT1: A novel regulator associated with the inflammatory response in acute respiratory distress syndrome. Gene, 2023 (PubMed: 37353041) [IF=2.6]

Application: WB    Species: human    Sample: A549 cells

Fig. 8. NEAT1 is involved in the inflammatory response in lung epithelial cells. (a) Wound scratch assay was performed and the changes of scratches were recorded at 0 h and 24 h. (b) Measurement and calculation of the migration rates were performed by image J. (c) Western blot was used to detect the expression of protein MMP9 and CXCL5 in A549 cells with NEAT1 suppression. (d-g) qPCR was utilized to analyze the expression of pro-inflammatory factors and miR-21-5p in A549 cells after LPS stimulation and NEAT1 knockdown. (h) Western blot was conducted to identify the expression of protein MMP9 and CXCL5 in A549 cells after LPS stimulation and NEAT1 knockdown. (i) The production of ROS was assessed by DCFH-DA probe. Representative white light images (upper) and fluorescence images (lower) were shown. (j-l) qPCR was utilized to analyze the expression of pro-inflammatory factors in A549 cells after miR-21-5p inhibition. Scale bar = 100 μm. Data were described with mean ± standard, n = 3. *p < 0.05 vs. the control group. **p < 0.01 vs. the control group. #p < 0.05 vs. the LPS group. ##p < 0.01 vs. the LPS group.
9). Upregulation of ITGB6 in primary palmar hyperhidrosis. Advances in clinical and experimental medicine : official organ Wroclaw Medical University, 2023 (PubMed: 37212774) [IF=2.1]

Application: WB    Species: Human    Sample:

Fig. 6. Regulatory effects of integrin β6 (ITGB6) on CXCL3, CXCL5, CXCL10, CXCL11, and WNT2 expression levels in sweat gland cells. Sweat gland cells extracted from patients with primary palmar hyperhidrosis were treated with blank control or transfected with overexpression negative control (OE NC) or ITGB6 OE. The CXCL3, CXCL5, CXCL10, CXCL11, and WNT2 (A) mRNA (ITGB6 OE compared to control, Tukey’s honest significant difference (HSD), p < 0.001, all), and (B,C) protein expression (ITGB6 OE compared to control, CXCL3, CXCL5, and CXCL11: Tukey’s HSD, all p < 0.001; CXCL10: Tukey’s HSD, p = 0.004; WNT2: Tukey’s HSD, p = 0.027). The data are presented as mean ± 95% confidence interval (95% CI)

限制条款

产品的规格、报价、验证数据请以官网为准，官网链接：www.affbiotech.com | www.affbiotech.cn（简体中文）| www.affbiotech.jp（日本語）

产品的数据信息为Affinity所有，未经授权不得收集Affinity官网数据或资料用于商业用途，对抄袭产品数据的行为我们将保留诉诸法律的权利。

产品相关数据会因产品批次、产品检测情况随时调整，如您已订购该产品，请以订购时随货说明书为准，否则请以官网内容为准，官网内容有改动时恕不另行通知。

Affinity保证所销售产品均经过严格质量检测。如您购买的商品在规定时间内出现问题需要售后时，请您在Affinity官方渠道提交售后申请。

产品仅供科学研究使用。不用于诊断和治疗。 

产品未经授权不得转售。

Affinity Biosciences将不会对在使用我们的产品时可能发生的专利侵权或其他侵权行为负责。Affinity Biosciences, Affinity Biosciences标志和所有其他商标所有权归Affinity Biosciences LTD.