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FIGURE 1 | The effect of TGEV on Na+ concentration and the relative expression of NHE3 in IPEC-J2 cells.

产品:	NHE-3 抗体
货号:	DF9937
描述:	Rabbit polyclonal antibody to NHE-3
应用:	WB
反应:	Human, Mouse, Rat
预测:	Pig, Bovine, Horse, Sheep, Rabbit, Dog, Chicken
分子量:	93kDa; 93kD(Calculated).
蛋白号:	P48764
RRID:	AB_2843131

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阻断肽(封闭肽)是特异性结合目标抗体和阻断抗体结合的多肽。这些多肽包含抗体识别的抗原表位。与阻断肽结合的抗体不再与靶蛋白上的表位结合。例如，在免疫印迹(WB)和免疫组化(IHC)中，通过比较被阻断抗体和未阻断抗体的染色，可以看到哪种染色是特异性的。

规格	价格	库存
100ul	RMB¥ 2300	现货
200ul	RMB¥ 3000	现货

货期: 当天发货

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实验方案

产品描述

来源:

Rabbit

应用:

WB 1:1000-3000
*The optimal dilutions should be determined by the end user.
*Tips:

WB: 适用于变性蛋白样本的免疫印迹检测. IHC: 适用于组织样本的石蜡(IHC-p)或冰冻(IHC-f)切片样本的免疫组化/荧光检测. IF/ICC: 适用于细胞样本的荧光检测. ELISA(peptide): 适用于抗原肽的ELISA检测.

反应:

Human,Mouse,Rat

预测:

Pig(100%), Bovine(100%), Horse(100%), Sheep(100%), Rabbit(100%), Dog(100%), Chicken(100%)

克隆:

Polyclonal

特异性:

NHE-3 Antibody detects endogenous levels of total NHE-3.

RRID:

AB_2843131
引用格式: Affinity Biosciences Cat# DF9937, RRID:AB_2843131.

偶联:

Unconjugated.

纯化:

The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).

保存:

Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.

别名:

展开/折叠

MGC126718; MGC126720; Na(+)/H(+) exchanger 3; NHE 3; NHE-3; NHE3; SL9A3_HUMAN; SLC9A 3; Slc9a3; Sodium/hydrogen exchanger 3; Sodium/hydrogen exchanger, apical epithelial; Solute carrier family 9 (sodium/hydrogen exchanger), isoform 3; Solute carrier family 9 (sodium/hydrogen exchanger), member 3; Solute carrier family 9 member 3;

抗原和靶标

免疫原:

Uniprot:

P48764(SL9A3_HUMAN) >>Visit HPA database.

基因/基因ID:

SLC9A3(6550)

序列:

P48764 SL9A3_HUMAN:
Protein BLAST With
NCBI/
ExPASy/
Uniprot

MWGLGARGPDRGLLLALALGGLARAGGVEVEPGGAHGESGGFQVVTFEWAHVQDPYVIALWILVASLAKIGFHLSHKVTSVVPESALLIVLGLVLGGIVWAADHIASFTLTPTVFFFYLLPPIVLDAGYFMPNRLFFGNLGTILLYAVVGTVWNAATTGLSLYGVFLSGLMGDLQIGLLDFLLFGSLMAAVDPVAVLAVFEEVHVNEVLFIIVFGESLLNDAVTVVLYNVFESFVALGGDNVTGVDCVKGIVSFFVVSLGGTLVGVVFAFLLSLVTRFTKHVRIIEPGFVFIISYLSYLTSEMLSLSAILAITFCGICCQKYVKANISEQSATTVRYTMKMLASSAETIIFMFLGISAVNPFIWTWNTAFVLLTLVFISVYRAIGVVLQTWLLNRYRMVQLEPIDQVVLSYGGLRGAVAFALVVLLDGDKVKEKNLFVSTTIIVVFFTVIFQGLTIKPLVQWLKVKRSEHREPRLNEKLHGRAFDHILSAIEDISGQIGHNYLRDKWSHFDRKFLSRVLMRRSAQKSRDRILNVFHELNLKDAISYVAEGERRGSLAFIRSPSTDNVVNVDFTPRSSTVEASVSYLLRENVSAVCLDMQSLEQRRRSIRDAEDMVTHHTLQQYLYKPRQEYKHLYSRHELTPTEDEKQDREIFHRTMRKRLESFKSTKLGLNQNKKAAKLYKRERAQKRRNSSIPNGKLPMESPAQNFTIKEKDLELSDTEEPPNYDEEMSGGIEFLASVTKDTASDSPAGIDNPVFSPDEALDRSLLARLPPWLSPGETVVPSQRARTQIPYSPGTFCRLMPFRLSSKSVDSFLQADGPEERPPAALPESTHM

种属预测

种属预测:

score>80的预测可信度较高，可尝试用于WB检测。*预测模型主要基于免疫原序列比对，结果仅作参考，不作为质保凭据。

Species

Results

Score

Pig

100

Horse

100

Bovine

100

Sheep

100

Dog

100

Chicken

100

Rabbit

100

Xenopus

Zebrafish

Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

翻译修饰 - P48764 作为底物

P48764

Site	PTM Type	Enzyme	Source
Y396	Phosphorylation		Uniprot
Y502	Phosphorylation		Uniprot
S545	Phosphorylation		Uniprot
S555	Phosphorylation		Uniprot
S563	Phosphorylation		Uniprot
T564	Phosphorylation		Uniprot
S592	Phosphorylation		Uniprot
S600	Phosphorylation		Uniprot
T641	Phosphorylation		Uniprot
S663	Phosphorylation	P51812 (RPS6KA3) , O00141 (SGK1)	Uniprot
K682	Acetylation		Uniprot
S794	Phosphorylation		Uniprot

研究背景

P48764_SL9A3_HUMAN

功能:

Involved in pH regulation to eliminate acids generated by active metabolism or to counter adverse environmental conditions. Major proton extruding system driven by the inward sodium ion chemical gradient. Plays an important role in signal transduction.

翻译修饰:

Phosphorylated by PKA, which inhibits activity. Phosphorylation at Ser-663 by SGK1 is associated with increased abundance at the cell membrane.

细胞定位:

Apical cell membrane>Multi-pass membrane protein.
Note: In intestinal epithelial cells, localizes to the ileal brush border. Phosphorylation at Ser-663 by SGK1 is associated with increased abundance at the cell membrane. Angiotensin-2 enhances apical expression (By similarity).

亚基结构:

Binds SLC9A3R1 and SLC9A3R2. Interacts with CHP1, CHP2 and SHANK2. Interacts with PDZK1 (via C-terminal PDZ domain) (By similarity). Interacts with PDZD3 and interactions decrease in response to elevated calcium ion levels. Interacts with AHCYL1; the interaction is required for SLC9A3 activity.

蛋白家族:

Belongs to the monovalent cation:proton antiporter 1 (CPA1) transporter (TC 2.A.36) family.

研究领域

· Organismal Systems > Excretory system > Proximal tubule bicarbonate reclamation.

· Organismal Systems > Digestive system > Protein digestion and absorption.

· Organismal Systems > Digestive system > Mineral absorption.

文献引用

1). Sodium butyrate ameliorates deoxycorticosterone acetate/salt-induced hypertension and renal damage by inhibiting the MR/SGK1 pathway. HYPERTENSION RESEARCH, 2021 (PubMed: 32908237) [IF=5.4]

Application: WB Species: rat Sample: renal cortex and medulla

Fig. 4 |Effects of NaBu on DOCA/salt-induced renal sodium transporter proteins.Representative immunoblots of α-ENaC, β-ENaC, γ-ENaC,NHE3, NCC, and NKCC-2 and summarized intensities of blots in the renal cortex (a) and medulla (b) (n = 6–7 per group).αENaC α-subunit of epithelial sodium channel, β-ENaC βsubunit of epithelial sodium channel, γ-ENaC γ-subunit of epithelial sodium channel,NHE3 sodium/proton exchanger isoform 3, NKCC-2 Na-K-2Cl cotransporter, NCC Na-Cl Cotransporter

2). EGFR as a Negative Regulatory Protein Adjusts the Activity and Mobility of NHE3 in the Cell Membrane of IPEC-J2 Cells With TGEV Infection. Frontiers in Microbiology, 2018 (PubMed: 30483239) [IF=5.2]

Application: WB Species: pig Sample: IPEC-J2 cells

FIGURE 1 | The effect of TGEV on Na+ concentration and the relative expression of NHE3 in IPEC-J2 cells. (A,B) Intra and extracellular Na+ concentration in cells infected with TGEV at 0, 48, and 72 h post infection. (C) NHE3 mRNA expression levels were detected using qRT-PCR and normalized by the β-actin mRNA level.(D)NHE3 protein levels were analyzed by Western blotting.

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NHE-3 Antibody - #DF9937

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