| 产品: | UBE4B 抗体 |
| 货号: | DF9997 |
| 描述: | Rabbit polyclonal antibody to UBE4B |
| 应用: | WB |
| 反应: | Human, Mouse |
| 预测: | Pig, Zebrafish, Bovine, Horse, Sheep, Rabbit, Dog, Chicken, Xenopus |
| 分子量: | 146 kDa; 146kD(Calculated). |
| 蛋白号: | O95155 |
| RRID: | AB_2843189 |
产品描述
*The optimal dilutions should be determined by the end user.
*Tips:
WB: 适用于变性蛋白样本的免疫印迹检测. IHC: 适用于组织样本的石蜡(IHC-p)或冰冻(IHC-f)切片样本的免疫组化/荧光检测. IF/ICC: 适用于细胞样本的荧光检测. ELISA(peptide): 适用于抗原肽的ELISA检测.
引用格式: Affinity Biosciences Cat# DF9997, RRID:AB_2843189.
展开/折叠
4930551I19Rik; 4933406G05Rik; AU014668; D4Bwg0973e; E4; HDNB1; Homozygously deleted in neuroblastoma 1; KIAA0684; mKIAA0684; RP23 173D8.1; Ube4b; UBE4B_HUMAN; Ubiquitin conjugation factor E4 B; Ubiquitin fusion degradation protein 2; UBOX3; UFD2; UFD2a; Ufd2p;
抗原和靶标
Expressed in differentiated myotubes (at protein level) (PubMed:17369820). Highest expression in ovary, testis, heart and skeletal muscle (PubMed:11802788). Expression is low in colon, thymus and peripheral blood leukocytes (PubMed:11802788). Almost undetectable in lung and spleen (PubMed:11802788).
- O95155 UBE4B_HUMAN:
- Protein BLAST With
- NCBI/
- ExPASy/
- Uniprot
MEELSADEIRRRRLARLAGGQTSQPTTPLTSPQRENPPGPPIAASAPGPSQSLGLNVHNMTPATSPIGASGVAHRSQSSEGVSSLSSSPSNSLETQSQSLSRSQSMDIDGVSCEKSMSQVDVDSGIENMEVDENDRREKRSLSDKEPSSGPEVSEEQALQLVCKIFRVSWKDRDRDVIFLSSLSAQFKQNPKEVFSDFKDLIGQILMEVLMMSTQTRDENPFASLTATSQPIAAAARSPDRNLLLNTGSNPGTSPMFCSVASFGASSLSSLYESSPAPTPSFWSSVPVMGPSLASPSRAASQLAVPSTPLSPHSAASGTAAGSQPSSPRYRPYTVTHPWASSGVSILSSSPSPPALASSPQAVPASSSRQRPSSTGPPLPPASPSATSRRPSSLRISPSLGASGGASNWDSYSDHFTIETCKETDMLNYLIECFDRVGIEEKKAPKMCSQPAVSQLLSNIRSQCISHTALVLQGSLTQPRSLQQPSFLVPYMLCRNLPYGFIQELVRTTHQDEEVFKQIFIPILQGLALAAKECSLDSDYFKYPLMALGELCETKFGKTHPVCNLVASLRLWLPKSLSPGCGRELQRLSYLGAFFSFSVFAEDDVKVVEKYFSGPAITLENTRVVSQSLQHYLELGRQELFKILHSILLNGETREAALSYMAAVVNANMKKAQMQTDDRLVSTDGFMLNFLWVLQQLSTKIKLETVDPTYIFHPRCRITLPNDETRVNATMEDVNDWLTELYGDQPPFSEPKFPTECFFLTLHAHHLSILPSCRRYIRRLRAIRELNRTVEDLKNNESQWKDSPLATRHREMLKRCKTQLKKLVRCKACADAGLLDESFLRRCLNFYGLLIQLLLRILDPAYPDITLPLNSDVPKVFAALPEFYVEDVAEFLFFIVQYSPQALYEPCTQDIVMFLVVMLCNQNYIRNPYLVAKLVEVMFMTNPAVQPRTQKFFEMIENHPLSTKLLVPSLMKFYTDVEHTGATSEFYDKFTIRYHISTIFKSLWQNIAHHGTFMEEFNSGKQFVRYINMLINDTTFLLDESLESLKRIHEVQEEMKNKEQWDQLPRDQQQARQSQLAQDERVSRSYLALATETVDMFHILTKQVQKPFLRPELGPRLAAMLNFNLQQLCGPKCRDLKVENPEKYGFEPKKLLDQLTDIYLQLDCARFAKAIADDQRSYSKELFEEVISKMRKAGIKSTIAIEKFKLLAEKVEEIVAKNARAEIDYSDAPDEFRDPLMDTLMTDPVRLPSGTIMDRSIILRHLLNSPTDPFNRQTLTESMLEPVPELKEQIQAWMREKQNSDH
种属预测
score>80的预测可信度较高，可尝试用于WB检测。*预测模型主要基于免疫原序列比对，结果仅作参考，不作为质保凭据。
High(score>80) Medium(80>score>50) Low(score<50) No confidence
翻译修饰 - O95155 作为底物
| Site | PTM Type | Enzyme | Source |
|---|---|---|---|
| M1 | Acetylation | Uniprot | |
| R10 | Methylation | Uniprot | |
| T22 | Phosphorylation | Uniprot | |
| S23 | Phosphorylation | Uniprot | |
| T26 | Phosphorylation | Uniprot | |
| T27 | Phosphorylation | Uniprot | |
| T30 | Phosphorylation | Uniprot | |
| S31 | Phosphorylation | Uniprot | |
| T61 | Phosphorylation | Uniprot | |
| T64 | Phosphorylation | Uniprot | |
| S65 | Phosphorylation | Uniprot | |
| S76 | Phosphorylation | Uniprot | |
| S78 | Phosphorylation | Uniprot | |
| S79 | Phosphorylation | Uniprot | |
| S83 | Phosphorylation | Uniprot | |
| S84 | Phosphorylation | Uniprot | |
| S86 | Phosphorylation | Uniprot | |
| S87 | Phosphorylation | Uniprot | |
| S88 | Phosphorylation | Uniprot | |
| S90 | Phosphorylation | Uniprot | |
| S92 | Phosphorylation | Uniprot | |
| T95 | Phosphorylation | Uniprot | |
| S99 | Phosphorylation | Uniprot | |
| S101 | Phosphorylation | Uniprot | |
| S103 | Phosphorylation | Uniprot | |
| S105 | Phosphorylation | Uniprot | |
| C113 | S-Nitrosylation | Uniprot | |
| S116 | Phosphorylation | Uniprot | |
| S118 | Phosphorylation | Uniprot | |
| S124 | Phosphorylation | Uniprot | |
| S141 | Phosphorylation | Uniprot | |
| S143 | Phosphorylation | Uniprot | |
| K145 | Ubiquitination | Uniprot | |
| S149 | Phosphorylation | Uniprot | |
| S154 | Phosphorylation | Uniprot | |
| K192 | Ubiquitination | Uniprot | |
| S224 | Phosphorylation | Uniprot | |
| S238 | Phosphorylation | Uniprot | |
| S307 | Phosphorylation | Uniprot | |
| S352 | Phosphorylation | Uniprot | |
| S383 | Phosphorylation | Uniprot | |
| S392 | Phosphorylation | Uniprot | |
| K442 | Ubiquitination | Uniprot | |
| K542 | Ubiquitination | Uniprot | |
| K558 | Ubiquitination | Uniprot | |
| K575 | Ubiquitination | Uniprot | |
| K610 | Ubiquitination | Uniprot | |
| K702 | Ubiquitination | Uniprot | |
| T705 | Phosphorylation | Uniprot | |
| T709 | Phosphorylation | Uniprot | |
| Y710 | Phosphorylation | Uniprot | |
| K794 | Ubiquitination | Uniprot | |
| S798 | Phosphorylation | Uniprot | |
| K801 | Sumoylation | Uniprot | |
| K801 | Ubiquitination | Uniprot | |
| S803 | Phosphorylation | Uniprot | |
| T807 | Phosphorylation | Uniprot | |
| K827 | Ubiquitination | Uniprot | |
| Y929 | Phosphorylation | Uniprot | |
| K951 | Ubiquitination | Uniprot | |
| K964 | Ubiquitination | Uniprot | |
| S969 | Phosphorylation | Uniprot | |
| K972 | Ubiquitination | Uniprot | |
| K989 | Ubiquitination | Uniprot | |
| Y1026 | Phosphorylation | Uniprot | |
| T1034 | Phosphorylation | Uniprot | |
| K1056 | Ubiquitination | Uniprot | |
| K1058 | Ubiquitination | Uniprot | |
| K1106 | Ubiquitination | Uniprot | |
| K1132 | Ubiquitination | Uniprot | |
| K1143 | Ubiquitination | Uniprot | |
| K1150 | Ubiquitination | Uniprot | |
| K1169 | Ubiquitination | Uniprot | |
| K1180 | Ubiquitination | Uniprot | |
| K1189 | Ubiquitination | Uniprot | |
| K1196 | Ubiquitination | Uniprot | |
| K1203 | Ubiquitination | Uniprot | |
| K1205 | Ubiquitination | Uniprot | |
| K1210 | Ubiquitination | Uniprot | |
| K1217 | Ubiquitination | Uniprot | |
| S1249 | Phosphorylation | Uniprot | |
| S1256 | Phosphorylation | Uniprot | |
| S1265 | Phosphorylation | Uniprot | |
| T1267 | Phosphorylation | Uniprot | |
| T1274 | Phosphorylation | Uniprot | |
| S1278 | Phosphorylation | Uniprot |
研究背景
Ubiquitin-protein ligase that probably functions as an E3 ligase in conjunction with specific E1 and E2 ligases (By similarity). May also function as an E4 ligase mediating the assembly of polyubiquitin chains on substrates ubiquitinated by another E3 ubiquitin ligase (By similarity). May regulate myosin assembly in striated muscles together with STUB1 and VCP/p97 by targeting myosin chaperone UNC45B for proteasomal degradation.
Proteolytically cleaved by caspases during apoptosis. Cleaved efficiently at Asp-123 by caspase-6 and granzyme B. Cleaved with approximately 10-fold less efficiency at Asp-109 by caspase-3 and caspase-7.
Cytoplasm. Nucleus.
Expressed in differentiated myotubes (at protein level). Highest expression in ovary, testis, heart and skeletal muscle. Expression is low in colon, thymus and peripheral blood leukocytes. Almost undetectable in lung and spleen.
Interacts with VCP/p97. Interacts with STUB1/CHIP and UNC45B.
The U-box domain is required for the ubiquitin protein ligase activity.
Belongs to the ubiquitin conjugation factor E4 family.
研究领域
· Genetic Information Processing > Folding, sorting and degradation > Ubiquitin mediated proteolysis. (View pathway)
· Genetic Information Processing > Folding, sorting and degradation > Protein processing in endoplasmic reticulum. (View pathway)
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