产品描述
*The optimal dilutions should be determined by the end user.
*Tips:
WB: 适用于变性蛋白样本的免疫印迹检测. IHC: 适用于组织样本的石蜡(IHC-p)或冰冻(IHC-f)切片样本的免疫组化/荧光检测. IF/ICC: 适用于细胞样本的荧光检测. ELISA(peptide): 适用于抗原肽的ELISA检测.
引用格式: Affinity Biosciences Cat# AF9034, RRID:AB_2843225.
展开/折叠
Casein kinase 1 gamma 2; Casein kinase I isoform gamma 1; Casein kinase I isoform gamma 3; Casein kinase I isoform gamma-2; CK1g2; CKI gamma 1; CKI gamma 2; CKI gamma 3; CSNK1G1; CSNK1G2; CSNK1G3;
抗原和靶标
- P78368 KC1G2_HUMAN:
- Protein BLAST With
- NCBI/
- ExPASy/
- Uniprot
MDFDKKGGKGETEEGRRMSKAGGGRSSHGIRSSGTSSGVLMVGPNFRVGKKIGCGNFGELRLGKNLYTNEYVAIKLEPIKSRAPQLHLEYRFYKQLSATEGVPQVYYFGPCGKYNAMVLELLGPSLEDLFDLCDRTFTLKTVLMIAIQLITRMEYVHTKSLIYRDVKPENFLVGRPGTKRQHAIHIIDFGLAKEYIDPETKKHIPYREHKSLTGTARYMSINTHLGKEQSRRDDLEALGHMFMYFLRGSLPWQGLKADTLKERYQKIGDTKRATPIEVLCENFPEEMATYLRYVRRLDFFEKPDYDYLRKLFTDLFDRSGFVFDYEYDWAGKPLPTPIGTVHTDLPSQPQLRDKTQPHSKNQALNSTNGELNADDPTAGHSNAPITAPAEVEVADETKCCCFFKRRKRKSLQRHK
种属预测
score>80的预测可信度较高，可尝试用于WB检测。*预测模型主要基于免疫原序列比对，结果仅作参考，不作为质保凭据。
High(score>80) Medium(80>score>50) Low(score<50) No confidence
翻译修饰 - P78368 作为底物
| Site | PTM Type | Enzyme | Source |
|---|---|---|---|
| R25 | Methylation | Uniprot | |
| S26 | Phosphorylation | Uniprot | |
| S32 | Phosphorylation | Uniprot | |
| S33 | Phosphorylation | Uniprot | |
| T35 | Phosphorylation | Uniprot | |
| S36 | Phosphorylation | Uniprot | |
| K51 | Ubiquitination | Uniprot | |
| K64 | Ubiquitination | Uniprot | |
| Y67 | Phosphorylation | Uniprot | |
| T68 | Phosphorylation | Uniprot | |
| Y71 | Phosphorylation | Uniprot | |
| Y90 | Phosphorylation | Uniprot | |
| Y93 | Phosphorylation | Uniprot | |
| T136 | Phosphorylation | Uniprot | |
| T138 | Phosphorylation | Uniprot | |
| T141 | Phosphorylation | Uniprot | |
| T151 | Phosphorylation | Uniprot | |
| T158 | Phosphorylation | Uniprot | |
| K167 | Ubiquitination | Uniprot | |
| Y195 | Phosphorylation | Uniprot | |
| K256 | Ubiquitination | Uniprot | |
| T259 | Phosphorylation | Uniprot | |
| K266 | Ubiquitination | Uniprot | |
| K310 | Ubiquitination | Uniprot | |
| T336 | Phosphorylation | Uniprot | |
| S359 | Phosphorylation | Uniprot | |
| S366 | Phosphorylation | Uniprot | |
| T367 | Phosphorylation | Uniprot | |
| T377 | Phosphorylation | Uniprot | |
| S381 | Phosphorylation | Uniprot | |
| K404 | Ubiquitination | Uniprot | |
| S410 | Phosphorylation | Uniprot |
翻译修饰 - P78368 作为激酶
研究背景
Serine/threonine-protein kinase. Casein kinases are operationally defined by their preferential utilization of acidic proteins such as caseins as substrates. It can phosphorylate a large number of proteins. Participates in Wnt signaling. Phosphorylates COL4A3BP/CERT, MTA1 and SMAD3. Involved in brain development and vesicular trafficking and neurotransmitter releasing from small synaptic vesicles. Regulates fast synaptic transmission mediated by glutamate. SMAD3 phosphorylation promotes its ligand-dependent ubiquitination and subsequent proteasome degradation, thus inhibiting SMAD3-mediated TGF-beta responses. Hyperphosphorylation of the serine-repeat motif of COL4A3BP/CERT leads to its inactivation by dissociation from the Golgi complex, thus down-regulating ER-to-Golgi transport of ceramide and sphingomyelin synthesis. Triggers PER1 proteasomal degradation probably through phosphorylation.
Autophosphorylated.
Cytoplasm.
Testis.
Monomer (By similarity). Interacts with MTA1 (short isoform) in the cytoplasm. Interacts with SMAD3.
Belongs to the protein kinase superfamily. CK1 Ser/Thr protein kinase family. Casein kinase I subfamily.
研究领域
· Environmental Information Processing > Signal transduction > Hedgehog signaling pathway. (View pathway)
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