| 产品: | Eg5 抗体 |
| 货号: | AF9058 |
| 描述: | Rabbit polyclonal antibody to Eg5 |
| 应用: | WB IF/ICC |
| 反应: | Human |
| 预测: | Pig, Horse, Rabbit, Dog |
| 分子量: | 119kDa; 119kD(Calculated). |
| 蛋白号: | P52732 |
| RRID: | AB_2843249 |
产品描述
*The optimal dilutions should be determined by the end user.
*Tips:
WB: 适用于变性蛋白样本的免疫印迹检测. IHC: 适用于组织样本的石蜡(IHC-p)或冰冻(IHC-f)切片样本的免疫组化/荧光检测. IF/ICC: 适用于细胞样本的荧光检测. ELISA(peptide): 适用于抗原肽的ELISA检测.
引用格式: Affinity Biosciences Cat# AF9058, RRID:AB_2843249.
展开/折叠
EG5; HKSP; KIF11; KIF11_HUMAN; Kinesin family member 11; Kinesin like protein 1; Kinesin-like protein 1; Kinesin-like protein KIF11; Kinesin-like spindle protein HKSP; Kinesin-related motor protein Eg5; KNSL1; MCLMR; Thyroid receptor-interacting protein 5; TR-interacting protein 5; TRIP-5; TRIP5;
抗原和靶标
- P52732 KIF11_HUMAN:
- Protein BLAST With
- NCBI/
- ExPASy/
- Uniprot
MASQPNSSAKKKEEKGKNIQVVVRCRPFNLAERKASAHSIVECDPVRKEVSVRTGGLADKSSRKTYTFDMVFGASTKQIDVYRSVVCPILDEVIMGYNCTIFAYGQTGTGKTFTMEGERSPNEEYTWEEDPLAGIIPRTLHQIFEKLTDNGTEFSVKVSLLEIYNEELFDLLNPSSDVSERLQMFDDPRNKRGVIIKGLEEITVHNKDEVYQILEKGAAKRTTAATLMNAYSSRSHSVFSVTIHMKETTIDGEELVKIGKLNLVDLAGSENIGRSGAVDKRAREAGNINQSLLTLGRVITALVERTPHVPYRESKLTRILQDSLGGRTRTSIIATISPASLNLEETLSTLEYAHRAKNILNKPEVNQKLTKKALIKEYTEEIERLKRDLAAAREKNGVYISEENFRVMSGKLTVQEEQIVELIEKIGAVEEELNRVTELFMDNKNELDQCKSDLQNKTQELETTQKHLQETKLQLVKEEYITSALESTEEKLHDAASKLLNTVEETTKDVSGLHSKLDRKKAVDQHNAEAQDIFGKNLNSLFNNMEELIKDGSSKQKAMLEVHKTLFGNLLSSSVSALDTITTVALGSLTSIPENVSTHVSQIFNMILKEQSLAAESKTVLQELINVLKTDLLSSLEMILSPTVVSILKINSQLKHIFKTSLTVADKIEDQKKELDGFLSILCNNLHELQENTICSLVESQKQCGNLTEDLKTIKQTHSQELCKLMNLWTERFCALEEKCENIQKPLSSVQENIQQKSKDIVNKMTFHSQKFCADSDGFSQELRNFNQEGTKLVEESVKHSDKLNGNLEKISQETEQRCESLNTRTVYFSEQWVSSLNEREQELHNLLEVVSQCCEASSSDITEKSDGRKAAHEKQHNIFLDQMTIDEDKLIAQNLELNETIKIGLTKLNCFLEQDLKLDIPTGTTPQRKSYLYPSTLVRTEPREHLLDQLKRKQPELLMMLNCSENNKEETIPDVDVEEAVLGQYTEEPLSQEPSVDAGVDCSSIGGVPFFQHKKSHGKDKENRGINTLERSKVEETTEHLVTKSRLPLRAQINL
种属预测
score>80的预测可信度较高，可尝试用于WB检测。*预测模型主要基于免疫原序列比对，结果仅作参考，不作为质保凭据。
High(score>80) Medium(80>score>50) Low(score<50) No confidence
翻译修饰 - P52732 作为底物
| Site | PTM Type | Enzyme | Source |
|---|---|---|---|
| K34 | Ubiquitination | Uniprot | |
| S36 | Phosphorylation | Uniprot | |
| K60 | Ubiquitination | Uniprot | |
| S62 | Phosphorylation | Uniprot | |
| T114 | Phosphorylation | Uniprot | |
| S120 | Phosphorylation | Uniprot | |
| Y125 | Phosphorylation | Uniprot | |
| T126 | Phosphorylation | Uniprot | |
| K146 | Acetylation | Uniprot | |
| K197 | Ubiquitination | Uniprot | |
| K207 | Ubiquitination | Uniprot | |
| Y211 | Phosphorylation | Uniprot | |
| K216 | Ubiquitination | Uniprot | |
| T223 | Phosphorylation | Uniprot | |
| T226 | Phosphorylation | Uniprot | |
| Y231 | Phosphorylation | Uniprot | |
| S232 | Phosphorylation | Uniprot | |
| S233 | Phosphorylation | Uniprot | |
| S240 | Phosphorylation | Uniprot | |
| K257 | Ubiquitination | Uniprot | |
| K260 | Ubiquitination | Uniprot | |
| S291 | Phosphorylation | Uniprot | |
| T294 | Phosphorylation | Uniprot | |
| S323 | Phosphorylation | Uniprot | |
| K357 | Ubiquitination | Uniprot | |
| K362 | Ubiquitination | Uniprot | |
| K372 | Acetylation | Uniprot | |
| K376 | Acetylation | Uniprot | |
| K376 | Ubiquitination | Uniprot | |
| K395 | Ubiquitination | Uniprot | |
| Y399 | Phosphorylation | Uniprot | |
| S401 | Phosphorylation | Uniprot | |
| K444 | Ubiquitination | Uniprot | |
| K451 | Ubiquitination | Uniprot | |
| K457 | Ubiquitination | Uniprot | |
| T458 | Phosphorylation | Uniprot | |
| T463 | Phosphorylation | Uniprot | |
| T464 | Phosphorylation | Uniprot | |
| K466 | Ubiquitination | Uniprot | |
| K472 | Ubiquitination | Uniprot | |
| K477 | Ubiquitination | Uniprot | |
| Y480 | Phosphorylation | Uniprot | |
| K491 | Ubiquitination | Uniprot | |
| K498 | Ubiquitination | Uniprot | |
| T506 | Phosphorylation | Uniprot | |
| T507 | Phosphorylation | Uniprot | |
| K508 | Ubiquitination | Uniprot | |
| K516 | Ubiquitination | Uniprot | |
| K521 | Ubiquitination | Uniprot | |
| K536 | Ubiquitination | Uniprot | |
| S540 | Phosphorylation | Uniprot | |
| K550 | Ubiquitination | Uniprot | |
| K618 | Ubiquitination | Uniprot | |
| T643 | Phosphorylation | Uniprot | |
| S646 | Phosphorylation | Uniprot | |
| S652 | Phosphorylation | Uniprot | |
| K655 | Ubiquitination | Uniprot | |
| K659 | Ubiquitination | Uniprot | |
| T663 | Phosphorylation | Uniprot | |
| K667 | Ubiquitination | Uniprot | |
| K712 | Ubiquitination | Uniprot | |
| K715 | Ubiquitination | Uniprot | |
| K724 | Ubiquitination | Uniprot | |
| K739 | Ubiquitination | Uniprot | |
| K745 | Ubiquitination | Uniprot | |
| K757 | Ubiquitination | Uniprot | |
| K764 | Ubiquitination | Uniprot | |
| K771 | Ubiquitination | Uniprot | |
| R784 | Methylation | Uniprot | |
| K792 | Ubiquitination | Uniprot | |
| K803 | Ubiquitination | Uniprot | |
| K810 | Ubiquitination | Uniprot | |
| S852 | Phosphorylation | Uniprot | |
| S858 | Phosphorylation | Uniprot | |
| K875 | Ubiquitination | Uniprot | |
| K890 | Ubiquitination | Uniprot | |
| K908 | Ubiquitination | Uniprot | |
| K918 | Ubiquitination | Uniprot | |
| T923 | Phosphorylation | Uniprot | |
| T925 | Phosphorylation | Uniprot | |
| T926 | Phosphorylation | P06493 (CDK1) | Uniprot |
| K930 | Ubiquitination | Uniprot | |
| S931 | Phosphorylation | Uniprot | |
| Y932 | Phosphorylation | Uniprot | |
| Y934 | Phosphorylation | Uniprot | |
| S936 | Phosphorylation | Uniprot | |
| K952 | Ubiquitination | Uniprot | |
| R1025 | Methylation | Uniprot | |
| T1029 | Phosphorylation | Uniprot | |
| S1033 | Phosphorylation | Q9HC98 (NEK6) | Uniprot |
| K1034 | Ubiquitination | Uniprot | |
| K1045 | Ubiquitination | Uniprot |
研究背景
Motor protein required for establishing a bipolar spindle during mitosis. Required in non-mitotic cells for transport of secretory proteins from the Golgi complex to the cell surface.
Phosphorylated exclusively on serine during S phase, but on both serine and Thr-926 during mitosis, so controlling the association of KIF11 with the spindle apparatus (probably during early prophase).
A subset of this protein primarily localized at the spindle pole is phosphorylated by NEK6 during mitosis; phosphorylation is required for mitotic function.
Cytoplasm. Cytoplasm>Cytoskeleton>Spindle pole.
Interacts with the thyroid hormone receptor in the presence of thyroid hormone. Component of a large chromatin remodeling complex, at least composed of MYSM1, PCAF, RBM10 and KIF11/TRIP5. Interacts (via C-terminus) with the kinase NEK6 in both interphase and mitosis.
Belongs to the TRAFAC class myosin-kinesin ATPase superfamily. Kinesin family. BimC subfamily.
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