产品描述
*The optimal dilutions should be determined by the end user.
*Tips:
WB: 适用于变性蛋白样本的免疫印迹检测. IHC: 适用于组织样本的石蜡(IHC-p)或冰冻(IHC-f)切片样本的免疫组化/荧光检测. IF/ICC: 适用于细胞样本的荧光检测. ELISA(peptide): 适用于抗原肽的ELISA检测.
引用格式: Affinity Biosciences Cat# AF9060, RRID:AB_2843251.
展开/折叠
EFL 6; EFL6; EFNB 3; Efnb3; EFNB3_HUMAN; EPH related receptor transmembrane ligand ELK L3; EPH related receptor transmembrane ligand ELKL3; EPH related receptor tyrosine kinase ligand 8; EPH-related receptor transmembrane ligand ELK-L3; EPH-related receptor tyrosine kinase ligand 8; Ephrin B3; Ephrin-B3; EphrinB3; EPLG 8; EPLG8; LERK 8; LERK-8; LERK8;
抗原和靶标
Highly expressed in brain; expressed in embryonic floor plate, roof plate and hindbrain segments.
- Q15768 EFNB3_HUMAN:
- Protein BLAST With
- NCBI/
- ExPASy/
- Uniprot
MGPPHSGPGGVRVGALLLLGVLGLVSGLSLEPVYWNSANKRFQAEGGYVLYPQIGDRLDLLCPRARPPGPHSSPNYEFYKLYLVGGAQGRRCEAPPAPNLLLTCDRPDLDLRFTIKFQEYSPNLWGHEFRSHHDYYIIATSDGTREGLESLQGGVCLTRGMKVLLRVGQSPRGGAVPRKPVSEMPMERDRGAAHSLEPGKENLPGDPTSNATSRGAEGPLPPPSMPAVAGAAGGLALLLLGVAGAGGAMCWRRRRAKPSESRHPGPGSFGRGGSLGLGGGGGMGPREAEPGELGIALRGGGAADPPFCPHYEKVSGDYGHPVYIVQDGPPQSPPNIYYKV
种属预测
score>80的预测可信度较高，可尝试用于WB检测。*预测模型主要基于免疫原序列比对，结果仅作参考，不作为质保凭据。
High(score>80) Medium(80>score>50) Low(score<50) No confidence
翻译修饰 - Q15768 作为底物
| Site | PTM Type | Enzyme | Source |
|---|---|---|---|
| S268 | Phosphorylation | Uniprot | |
| S274 | Phosphorylation | Uniprot | |
| Y311 | Phosphorylation | Uniprot | |
| Y318 | Phosphorylation | Uniprot | |
| Y323 | Phosphorylation | Uniprot | |
| S332 | Phosphorylation | Uniprot | |
| Y337 | Phosphorylation | Uniprot | |
| Y338 | Phosphorylation | Uniprot |
研究背景
Cell surface transmembrane ligand for Eph receptors, a family of receptor tyrosine kinases which are crucial for migration, repulsion and adhesion during neuronal, vascular and epithelial development. Binds promiscuously Eph receptors residing on adjacent cells, leading to contact-dependent bidirectional signaling into neighboring cells. The signaling pathway downstream of the receptor is referred to as forward signaling while the signaling pathway downstream of the ephrin ligand is referred to as reverse signaling. May play a pivotal role in forebrain function. Binds to, and induce the collapse of, commissural axons/growth cones in vitro. May play a role in constraining the orientation of longitudinally projecting axons (By similarity).
(Microbial infection) Acts as a receptor for nipah virus and hendra virus.
Membrane>Single-pass type I membrane protein.
Highly expressed in brain; expressed in embryonic floor plate, roof plate and hindbrain segments.
Interacts with GRIP1 and GRIP2.
(Microbial infection) Interacts with nipah virus and hendra virus glycoprotein.
Belongs to the ephrin family.
研究领域
· Organismal Systems > Development > Axon guidance. (View pathway)
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