| 产品: | eRF3/GSPT1 抗体 |
| 货号: | AF9062 |
| 描述: | Rabbit polyclonal antibody to eRF3/GSPT1 |
| 应用: | WB IHC |
| 反应: | Human, Mouse |
| 预测: | Pig, Bovine, Horse, Sheep, Rabbit, Dog, Xenopus |
| 分子量: | 55kDa; 56kD(Calculated). |
| 蛋白号: | P15170 |
| RRID: | AB_2843253 |
产品描述
*The optimal dilutions should be determined by the end user.
*Tips:
WB: 适用于变性蛋白样本的免疫印迹检测. IHC: 适用于组织样本的石蜡(IHC-p)或冰冻(IHC-f)切片样本的免疫组化/荧光检测. IF/ICC: 适用于细胞样本的荧光检测. ELISA(peptide): 适用于抗原肽的ELISA检测.
引用格式: Affinity Biosciences Cat# AF9062, RRID:AB_2843253.
展开/折叠
551G9.2; Anti Eukaryotic Release Factor 3a; Anti G1 to S phase transition 1; Anti GST1, homolog of yeast; eRF 3a; eRF3a; G1 to S phase transition 1; G1 to S phase transition protein 1 homolog; G1 to S phase transition protein; GSPT 1; GSPT1; GST 1; GST1; GTP binding protein GST1 HS; Transition protein 1;
抗原和靶标
- P15170 ERF3A_HUMAN:
- Protein BLAST With
- NCBI/
- ExPASy/
- Uniprot
MELSEPIVENGETEMSPEESWEHKEEISEAEPGGGSLGDGRPPEESAHEMMEEEEEIPKPKSVVAPPGAPKKEHVNVVFIGHVDAGKSTIGGQIMYLTGMVDKRTLEKYEREAKEKNRETWYLSWALDTNQEERDKGKTVEVGRAYFETEKKHFTILDAPGHKSFVPNMIGGASQADLAVLVISARKGEFETGFEKGGQTREHAMLAKTAGVKHLIVLINKMDDPTVNWSNERYEECKEKLVPFLKKVGFNPKKDIHFMPCSGLTGANLKEQSDFCPWYIGLPFIPYLDNLPNFNRSVDGPIRLPIVDKYKDMGTVVLGKLESGSICKGQQLVMMPNKHNVEVLGILSDDVETDTVAPGENLKIRLKGIEEEEILPGFILCDPNNLCHSGRTFDAQIVIIEHKSIICPGYNAVLHIHTCIEEVEITALICLVDKKSGEKSKTRPRFVKQDQVCIARLRTAGTICLETFKDFPQMGRFTLRDEGKTIAIGKVLKLVPEKD
种属预测
score>80的预测可信度较高，可尝试用于WB检测。*预测模型主要基于免疫原序列比对，结果仅作参考，不作为质保凭据。
High(score>80) Medium(80>score>50) Low(score<50) No confidence
翻译修饰 - P15170 作为底物
| Site | PTM Type | Enzyme | Source |
|---|---|---|---|
| S16 | Phosphorylation | Uniprot | |
| S20 | Phosphorylation | Uniprot | |
| S36 | Phosphorylation | Uniprot | |
| S46 | Phosphorylation | Uniprot | |
| S62 | Phosphorylation | Uniprot | |
| K71 | Ubiquitination | Uniprot | |
| K108 | Acetylation | Uniprot | |
| K108 | Ubiquitination | Uniprot | |
| Y109 | Phosphorylation | Uniprot | |
| T120 | Phosphorylation | Uniprot | |
| T129 | Phosphorylation | Uniprot | |
| K138 | Ubiquitination | Uniprot | |
| Y146 | Phosphorylation | Uniprot | |
| K152 | Ubiquitination | Uniprot | |
| K187 | Ubiquitination | Uniprot | |
| T192 | Phosphorylation | Uniprot | |
| K196 | Acetylation | Uniprot | |
| K196 | Methylation | Uniprot | |
| K196 | Ubiquitination | Uniprot | |
| K208 | Acetylation | Uniprot | |
| K208 | Ubiquitination | Uniprot | |
| K238 | Acetylation | Uniprot | |
| K240 | Ubiquitination | Uniprot | |
| K253 | Acetylation | Uniprot | |
| K253 | Ubiquitination | Uniprot | |
| T315 | Phosphorylation | Uniprot | |
| K320 | Ubiquitination | Uniprot | |
| K328 | Ubiquitination | Uniprot | |
| K363 | Ubiquitination | Uniprot | |
| K367 | Ubiquitination | Uniprot | |
| K448 | Ubiquitination | Uniprot | |
| C453 | S-Nitrosylation | Uniprot | |
| K469 | Acetylation | Uniprot | |
| K469 | Ubiquitination | Uniprot | |
| T478 | Phosphorylation | Uniprot | |
| K484 | Ubiquitination | Uniprot | |
| K490 | Acetylation | Uniprot | |
| K490 | Ubiquitination | Uniprot | |
| K493 | Acetylation | Uniprot | |
| K493 | Ubiquitination | Uniprot |
研究背景
Involved in translation termination in response to the termination codons UAA, UAG and UGA (By similarity). Stimulates the activity of ETF1 (By similarity). Involved in regulation of mammalian cell growth. Component of the transient SURF complex which recruits UPF1 to stalled ribosomes in the context of nonsense-mediated decay (NMD) of mRNAs containing premature stop codons. Required for SHFL-mediated translation termination which inhibits programmed ribosomal frameshifting (-1PRF) of mRNA from viruses and cellular genes.
Component of the transient SURF (SMG1-UPF1-eRF1-eRF3) complex. The ETF1-GSPT1 complex interacts with JMJD4. Interacts with PABPC1 (By similarity). Interacts with SHFL.
Belongs to the TRAFAC class translation factor GTPase superfamily. Classic translation factor GTPase family. ERF3 subfamily.
研究领域
· Genetic Information Processing > Translation > mRNA surveillance pathway.
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