Glucosidase II alpha Antibody - #AF9072

(2)  
产品: Glucosidase II alpha 抗体
货号: AF9072
描述: Rabbit polyclonal antibody to Glucosidase II alpha
应用: WB IHC IF/ICC
反应: Human, Mouse
预测: Pig, Zebrafish, Bovine, Horse, Sheep, Rabbit, Dog, Xenopus
分子量: 107kDa; 107kD(Calculated).
蛋白号: Q14697
RRID: AB_2843263

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 50ul RMB¥ 1250 现货
 100ul RMB¥ 2300 现货
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MS Report
HPLC Report
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说明书
COA
实验方案
WB Handbook
IHC Protocol
IF/ICC Protocol

产品描述

来源:
Rabbit
应用:
WB 1:1000, IF/ICC 1:100-1:500, IHC 1:50-1:200
*The optimal dilutions should be determined by the end user.
*Tips:

WB: 适用于变性蛋白样本的免疫印迹检测. IHC: 适用于组织样本的石蜡(IHC-p)或冰冻(IHC-f)切片样本的免疫组化/荧光检测. IF/ICC: 适用于细胞样本的荧光检测. ELISA(peptide): 适用于抗原肽的ELISA检测.

反应:
Human,Mouse
预测:
Pig(91%), Zebrafish(80%), Bovine(91%), Horse(91%), Sheep(91%), Rabbit(100%), Dog(91%), Xenopus(100%)
克隆:
Polyclonal
特异性:
Glucosidase II alpha Antibody detects endogenous levels of total Glucosidase II alpha.
RRID:
AB_2843263
引用格式: Affinity Biosciences Cat# AF9072, RRID:AB_2843263.
偶联:
Unconjugated.
纯化:
The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).
保存:
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
别名:

展开/折叠

Alpha glucosidase II alpha subunit; Alpha-glucosidase 2; G2AN; GANAB; GANAB_HUMAN; Glu II; Glucosidase alpha neutral AB; Glucosidase II alpha; Glucosidase II subunit alpha; GluII; KIAA0088; Neutral alpha glucosidase AB; Neutral alpha glucosidase AB precursor; Neutral alpha-glucosidase AB;

抗原和靶标

免疫原:
Uniprot:

Q14697(GANAB_HUMAN) >>Visit HPA database.

基因/基因ID:
GANAB(23193)
表达:
Q14697 GANAB_HUMAN:

Detected in placenta (PubMed:3881423). Isoform 1 and isoform 2 are expressed in the kidney and liver (PubMed:27259053).

序列:
MAAVAAVAARRRRSWASLVLAFLGVCLGITLAVDRSNFKTCEESSFCKRQRSIRPGLSPYRALLDSLQLGPDSLTVHLIHEVTKVLLVLELQGLQKNMTRFRIDELEPRRPRYRVPDVLVADPPIARLSVSGRDENSVELTMAEGPYKIILTARPFRLDLLEDRSLLLSVNARGLLEFEHQRAPRVSQGSKDPAEGDGAQPEETPRDGDKPEETQGKAEKDEPGAWEETFKTHSDSKPYGPMSVGLDFSLPGMEHVYGIPEHADNLRLKVTEGGEPYRLYNLDVFQYELYNPMALYGSVPVLLAHNPHRDLGIFWLNAAETWVDISSNTAGKTLFGKMMDYLQGSGETPQTDVRWMSETGIIDVFLLLGPSISDVFRQYASLTGTQALPPLFSLGYHQSRWNYRDEADVLEVDQGFDDHNLPCDVIWLDIEHADGKRYFTWDPSRFPQPRTMLERLASKRRKLVAIVDPHIKVDSGYRVHEELRNLGLYVKTRDGSDYEGWCWPGSAGYPDFTNPTMRAWWANMFSYDNYEGSAPNLFVWNDMNEPSVFNGPEVTMLKDAQHYGGWEHRDVHNIYGLYVHMATADGLRQRSGGMERPFVLARAFFAGSQRFGAVWTGDNTAEWDHLKISIPMCLSLGLVGLSFCGADVGGFFKNPEPELLVRWYQMGAYQPFFRAHAHLDTGRREPWLLPSQHNDIIRDALGQRYSLLPFWYTLLYQAHREGIPVMRPLWVQYPQDVTTFNIDDQYLLGDALLVHPVSDSGAHGVQVYLPGQGEVWYDIQSYQKHHGPQTLYLPVTLSSIPVFQRGGTIVPRWMRVRRSSECMKDDPITLFVALSPQGTAQGELFLDDGHTFNYQTRQEFLLRRFSFSGNTLVSSSADPEGHFETPIWIERVVIIGAGKPAAVVLQTKGSPESRLSFQHDPETSVLVLRKPGINVASDWSIHLR

种属预测

种属预测:

score>80的预测可信度较高，可尝试用于WB检测。*预测模型主要基于免疫原序列比对，结果仅作参考，不作为质保凭据。

Species
Results
Score
Rabbit
100
Xenopus
100
Pig
91
Horse
91
Bovine
91
Sheep
91
Dog
91
Zebrafish
80
Chicken
0
Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

翻译修饰 - Q14697 作为底物

Site PTM Type Enzyme
S36 Phosphorylation
K39 Ubiquitination
T40 Phosphorylation
C47 S-Nitrosylation
K48 Acetylation
K48 Methylation
K48 Ubiquitination
S52 Phosphorylation
S58 Phosphorylation
Y60 Phosphorylation
N97 N-Glycosylation
T141 Phosphorylation
R164 Methylation
S165 Phosphorylation
R182 Methylation
S190 Phosphorylation
K191 Ubiquitination
T204 Phosphorylation
R206 Methylation
K217 Ubiquitination
K220 Ubiquitination
K231 Ubiquitination
K237 Ubiquitination
K269 Ubiquitination
K337 Ubiquitination
Y341 Phosphorylation
S345 Phosphorylation
T348 Phosphorylation
T351 Phosphorylation
Y379 Phosphorylation
Y396 Phosphorylation
K436 Acetylation
K462 Ubiquitination
K472 Sumoylation
K472 Ubiquitination
R484 Methylation
Y489 Phosphorylation
K491 Ubiquitination
Y563 Phosphorylation
Y578 Phosphorylation
R590 Methylation
S608 Phosphorylation
R698 Methylation
T790 Phosphorylation
Y792 Phosphorylation
T796 Phosphorylation
T808 Phosphorylation
S820 Phosphorylation
R863 Methylation
S866 Phosphorylation
S874 Phosphorylation
S876 Phosphorylation
K899 Ubiquitination
K908 Ubiquitination
K930 Ubiquitination
S937 Phosphorylation
R944 Methylation

研究背景

功能:

Catalytic subunit of glucosidase II that cleaves sequentially the 2 innermost alpha-1,3-linked glucose residues from the Glc(2)Man(9)GlcNAc(2) oligosaccharide precursor of immature glycoproteins. Required for PKD1/Polycystin-1 and PKD2/Polycystin-2 maturation and localization to the cell surface and cilia.

细胞定位:

Endoplasmic reticulum. Golgi apparatus. Melanosome.
Note: Identified by mass spectrometry in melanosome fractions from stage I to stage IV.

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionSubcellular location
组织特异性:

Detected in placenta. Isoform 1 and isoform 2 are expressed in the kidney and liver.

亚基结构:

Heterodimer of a catalytic alpha subunit (GANAB) and a beta subunit (PRKCSH). Binds glycosylated PTPRC (By similarity).

蛋白家族:

Belongs to the glycosyl hydrolase 31 family.

研究领域

· Genetic Information Processing > Folding, sorting and degradation > Protein processing in endoplasmic reticulum.   (View pathway)

· Metabolism > Glycan biosynthesis and metabolism > N-Glycan biosynthesis.

· Metabolism > Global and overview maps > Metabolic pathways.

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