产品: LHR 抗体
货号: AF9104
描述: Rabbit polyclonal antibody to LHR
应用: WB IHC IF/ICC
反应: Human, Mouse
预测: Dog
分子量: 80kDa; 79kD(Calculated).
蛋白号: P22888
RRID: AB_2843294

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   规格 价格 库存
 50ul RMB¥ 1250 现货
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 200ul RMB¥ 3000 现货

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产品描述

来源:
Rabbit
应用:
WB 1:1000, IF/ICC 1:100-1:500, IHC 1:50-1:200
*The optimal dilutions should be determined by the end user.
*Tips:

WB: 适用于变性蛋白样本的免疫印迹检测. IHC: 适用于组织样本的石蜡(IHC-p)或冰冻(IHC-f)切片样本的免疫组化/荧光检测. IF/ICC: 适用于细胞样本的荧光检测. ELISA(peptide): 适用于抗原肽的ELISA检测.

反应:
Human,Mouse
预测:
Dog(89%)
克隆:
Polyclonal
特异性:
LHR Antibody detects endogenous levels of total LHR.
RRID:
AB_2843294
引用格式: Affinity Biosciences Cat# AF9104, RRID:AB_2843294.
偶联:
Unconjugated.
纯化:
The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).
保存:
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
别名:

展开/折叠

Gonadotropin receptor; Gpcr19-rs1; GTHR-II; HHG; LCGR; LGR2; LH-R; LH/CG R; LH/CG-R; LH/CGR; LHCGR; LHR; LHRHR; LSH R; LSH-R; LSHR_HUMAN; Luteinizing hormone receptor; Luteinizing hormone/choriogonadotropin receptor; Lutropin choriogonadotropic hormone receptor; Lutropin choriogonadotropic receptor; Lutropin-choriogonadotropic hormone receptor; ULG5;

抗原和靶标

免疫原:
Uniprot:
基因/基因ID:
表达:
P22888 LSHR_HUMAN:

Gonadal and thyroid cells.

序列:
MKQRFSALQLLKLLLLLQPPLPRALREALCPEPCNCVPDGALRCPGPTAGLTRLSLAYLPVKVIPSQAFRGLNEVIKIEISQIDSLERIEANAFDNLLNLSEILIQNTKNLRYIEPGAFINLPRLKYLSICNTGIRKFPDVTKVFSSESNFILEICDNLHITTIPGNAFQGMNNESVTLKLYGNGFEEVQSHAFNGTTLTSLELKENVHLEKMHNGAFRGATGPKTLDISSTKLQALPSYGLESIQRLIATSSYSLKKLPSRETFVNLLEATLTYPSHCCAFRNLPTKEQNFSHSISENFSKQCESTVRKVNNKTLYSSMLAESELSGWDYEYGFCLPKTPRCAPEPDAFNPCEDIMGYDFLRVLIWLINILAIMGNMTVLFVLLTSRYKLTVPRFLMCNLSFADFCMGLYLLLIASVDSQTKGQYYNHAIDWQTGSGCSTAGFFTVFASELSVYTLTVITLERWHTITYAIHLDQKLRLRHAILIMLGGWLFSSLIAMLPLVGVSNYMKVSICFPMDVETTLSQVYILTILILNVVAFFIICACYIKIYFAVRNPELMATNKDTKIAKKMAILIFTDFTCMAPISFFAISAAFKVPLITVTNSKVLLVLFYPINSCANPFLYAIFTKTFQRDFFLLLSKFGCCKRRAELYRRKDFSAYTSNCKNGFTGSNKPSQSTLKLSTLHCQGTALLDKTRYTEC

种属预测

种属预测:

score>80的预测可信度较高,可尝试用于WB检测。*预测模型主要基于免疫原序列比对,结果仅作参考,不作为质保凭据。

Species
Results
Score
Dog
89
Pig
78
Bovine
78
Sheep
78
Chicken
78
Rabbit
78
Xenopus
71
Horse
0
Zebrafish
0
Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

翻译修饰 - P22888 作为底物

Site PTM Type Enzyme
S494 Phosphorylation
S681 Phosphorylation
T688 Phosphorylation

研究背景

功能:

Receptor for lutropin-choriogonadotropic hormone. The activity of this receptor is mediated by G proteins which activate adenylate cyclase.

翻译修饰:

Sulfated.

细胞定位:

Cell membrane>Multi-pass membrane protein.

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionSubcellular location
组织特异性:

Gonadal and thyroid cells.

蛋白家族:

Belongs to the G-protein coupled receptor 1 family. FSH/LSH/TSH subfamily.

研究领域

· Environmental Information Processing > Signal transduction > Calcium signaling pathway.   (View pathway)

· Environmental Information Processing > Signaling molecules and interaction > Neuroactive ligand-receptor interaction.

· Organismal Systems > Endocrine system > Ovarian steroidogenesis.

· Organismal Systems > Endocrine system > Prolactin signaling pathway.   (View pathway)

文献引用

1). Study on the changes of LHR, FSHR and AR with the development of testis cells in Hu sheep. Animal Reproduction Science, 2023 (PubMed: 37541020) [IF=2.2]

2). miR-17-5p affects porcine granulosa cell growth and estradiol synthesis by targeting E2F1 gene. REPRODUCTION IN DOMESTIC ANIMALS, 2019 (PubMed: 31424586) [IF=1.7]

Application: WB    Species: porcine    Sample: porcine GCs

FIGURE 3 |miR‐17‐5p up‐regulates LHR, CYP19A1 and AREG expression and oestradiol synthesis in pGCs. All the experiments were done in porcine GCs 48 hr after transfected. (a) The mRNA expression levels of LHR, CYP19A1 and AREG in pGCs transfected with miR‐17‐5p mimics or mimics NC were detected by qRT‐PCR. (b) Protein levels of LHR, CYP19A1 and AREG in pGCs transfected with miR‐17‐5p mimics or mimics NC were detected by Western blot.

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