MINK1 Antibody - #AF9112

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产品: MINK1 抗体
货号: AF9112
描述: Rabbit polyclonal antibody to MINK1
应用: WB IHC IF/ICC
反应: Human
预测: Pig, Zebrafish, Bovine, Horse, Dog, Xenopus
分子量: 150kDa; 150kD(Calculated).
蛋白号: Q8N4C8
RRID: AB_2843302

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   规格 价格 库存
 50ul RMB¥ 1250 现货
 100ul RMB¥ 2300 现货
 200ul RMB¥ 3000 现货

货期: 当天发货

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相关下载
MS Report
HPLC Report
MSDS
说明书
COA
实验方案
WB Handbook
IHC Protocol
IF/ICC Protocol

产品描述

来源:
Rabbit
应用:
IHC 1:50-1:200, WB 1:1000, IF/ICC 1:100-1:500
*The optimal dilutions should be determined by the end user.
*Tips:

WB: 适用于变性蛋白样本的免疫印迹检测. IHC: 适用于组织样本的石蜡(IHC-p)或冰冻(IHC-f)切片样本的免疫组化/荧光检测. IF/ICC: 适用于细胞样本的荧光检测. ELISA(peptide): 适用于抗原肽的ELISA检测.

反应:
Human
预测:
Pig(100%), Zebrafish(82%), Bovine(100%), Horse(100%), Dog(100%), Xenopus(100%)
克隆:
Polyclonal
特异性:
MINK1 Antibody detects endogenous levels of total MINK1.
RRID:
AB_2843302
引用格式: Affinity Biosciences Cat# AF9112, RRID:AB_2843302.
偶联:
Unconjugated.
纯化:
The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).
保存:
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
别名:

展开/折叠

B55; GCK family kinase MiNK; hMINK; Map4k6; MAPK/ERK kinase kinase kinase 6; MEK (MAP/ERK) kinase kinase 6; MEK kinase kinase 6; MEKKK6; MGC21111; MINK 1; MINK; MINK1; Misshapen like kinase 1; Misshapen NIKs Related kinase; Misshapen/NIK related kinase; Mitogen activated protein kinase kinase kinase kinase 6; RP23-122P1.6; Ysk2; ZC3;

抗原和靶标

免疫原:
Uniprot:

Q8N4C8(MINK1_HUMAN) >>Visit HPA database.

基因/基因ID:
MINK1(50488)
表达:
Q8N4C8 MINK1_HUMAN:

Expressed in the brain, isoform 2 is more abundant than isoform 1. Isoform 3 is ubiquitously expressed. Isoform 1 is most abundant in the skeletal muscle. Isoform 4 is ubiquitously expressed with relative high levels in brain, skeletal muscle, pancreas and testis.

序列:
MGDPAPARSLDDIDLSALRDPAGIFELVEVVGNGTYGQVYKGRHVKTGQLAAIKVMDVTEDEEEEIKQEINMLKKYSHHRNIATYYGAFIKKSPPGNDDQLWLVMEFCGAGSVTDLVKNTKGNALKEDCIAYICREILRGLAHLHAHKVIHRDIKGQNVLLTENAEVKLVDFGVSAQLDRTVGRRNTFIGTPYWMAPEVIACDENPDATYDYRSDIWSLGITAIEMAEGAPPLCDMHPMRALFLIPRNPPPRLKSKKWSKKFIDFIDTCLIKTYLSRPPTEQLLKFPFIRDQPTERQVRIQLKDHIDRSRKKRGEKEETEYEYSGSEEEDDSHGEEGEPSSIMNVPGESTLRREFLRLQQENKSNSEALKQQQQLQQQQQRDPEAHIKHLLHQRQRRIEEQKEERRRVEEQQRREREQRKLQEKEQQRRLEDMQALRREEERRQAEREQEYKRKQLEEQRQSERLQRQLQQEHAYLKSLQQQQQQQQLQKQQQQQLLPGDRKPLYHYGRGMNPADKPAWAREVEERTRMNKQQNSPLAKSKPGSTGPEPPIPQASPGPPGPLSQTPPMQRPVEPQEGPHKSLVAHRVPLKPYAAPVPRSQSLQDQPTRNLAAFPASHDPDPAIPAPTATPSARGAVIRQNSDPTSEGPGPSPNPPAWVRPDNEAPPKVPQRTSSIATALNTSGAGGSRPAQAVRARPRSNSAWQIYLQRRAERGTPKPPGPPAQPPGPPNASSNPDLRRSDPGWERSDSVLPASHGHLPQAGSLERNRVGVSSKPDSSPVLSPGNKAKPDDHRSRPGRPADFVLLKERTLDEAPRPPKKAMDYSSSSEEVESSEDDEEEGEGGPAEGSRDTPGGRSDGDTDSVSTMVVHDVEEITGTQPPYGGGTMVVQRTPEEERNLLHADSNGYTNLPDVVQPSHSPTENSKGQSPPSKDGSGDYQSRGLVKAPGKSSFTMFVDLGIYQPGGSGDSIPITALVGGEGTRLDQLQYDVRKGSVVNVNPTNTRAHSETPEIRKYKKRFNSEILCAALWGVNLLVGTENGLMLLDRSGQGKVYGLIGRRRFQQMDVLEGLNLLITISGKRNKLRVYYLSWLRNKILHNDPEVEKKQGWTTVGDMEGCGHYRVVKYERIKFLVIALKSSVEVYAWAPKPYHKFMAFKSFADLPHRPLLVDLTVEEGQRLKVIYGSSAGFHAVDVDSGNSYDIYIPVHIQSQITPHAIIFLPNTDGMEMLLCYEDEGVYVNTYGRIIKDVVLQWGEMPTSVAYICSNQIMGWGEKAIEIRSVETGHLDGVFMHKRAQRLKFLCERNDKVFFASVRSGGSSQVYFMTLNRNCIMNW

种属预测

种属预测:

score>80的预测可信度较高，可尝试用于WB检测。*预测模型主要基于免疫原序列比对，结果仅作参考，不作为质保凭据。

Species
Results
Score
Pig
100
Horse
100
Bovine
100
Dog
100
Xenopus
100
Zebrafish
82
Chicken
67
Rabbit
64
Sheep
0
Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

翻译修饰 - Q8N4C8 作为底物

Site PTM Type Enzyme
S9 Phosphorylation
S16 Phosphorylation
Y36 Phosphorylation
K46 Ubiquitination
K54 Ubiquitination
T59 Phosphorylation
Y86 Phosphorylation
K155 Ubiquitination
S175 Phosphorylation
T181 Phosphorylation
T187 Phosphorylation Q8N4C8 (MINK1)
T191 Phosphorylation Q8N4C8 (MINK1)
Y193 Phosphorylation Q8N4C8 (MINK1)
T280 Phosphorylation O14757 (CHEK1)
K303 Ubiquitination
Y321 Phosphorylation
Y323 Phosphorylation
S324 Phosphorylation
S326 Phosphorylation
S332 Phosphorylation
K363 Ubiquitination
K370 Ubiquitination
K388 Ubiquitination
Y475 Phosphorylation
K477 Ubiquitination
S478 Phosphorylation
K490 Ubiquitination
Y505 Phosphorylation
Y507 Phosphorylation
R509 Methylation
S535 Phosphorylation
S563 Phosphorylation
T565 Phosphorylation
S581 Phosphorylation
K590 Acetylation
K590 Methylation
Y592 Phosphorylation
S601 Phosphorylation
T629 Phosphorylation
S641 Phosphorylation
T644 Phosphorylation
S673 Phosphorylation
S674 Phosphorylation
S699 Phosphorylation
S701 Phosphorylation
Y706 Phosphorylation
S732 Phosphorylation
S733 Phosphorylation
S740 Phosphorylation
S747 Phosphorylation
S749 Phosphorylation
S754 Phosphorylation
S763 Phosphorylation
S772 Phosphorylation
S773 Phosphorylation
S777 Phosphorylation
S778 Phosphorylation
S782 Phosphorylation
T809 Phosphorylation
S903 Phosphorylation
Y906 Phosphorylation
T907 Phosphorylation
S916 Phosphorylation
S918 Phosphorylation
T920 Phosphorylation
S927 Phosphorylation
K931 Ubiquitination
Y937 Phosphorylation
S939 Phosphorylation
Y960 Phosphorylation
Y987 Phosphorylation
S993 Phosphorylation
T1008 Phosphorylation
S1088 Phosphorylation
K1103 Ubiquitination
K1146 Ubiquitination
K1150 Ubiquitination
K1291 Ubiquitination
K1297 Ubiquitination
K1305 Ubiquitination
S1317 Phosphorylation
Y1320 Phosphorylation
T1323 Phosphorylation

研究背景

功能:

Serine/threonine kinase which acts as a negative regulator of Ras-related Rap2-mediated signal transduction to control neuronal structure and AMPA receptor trafficking. Required for normal synaptic density, dendrite complexity, as well as surface AMPA receptor expression in hippocampal neurons. Can activate the JNK and MAPK14/p38 pathways and mediates stimulation of the stress-activated protein kinase MAPK14/p38 MAPK downstream of the Raf/ERK pathway. Phosphorylates: TANC1 upon stimulation by RAP2A, MBP and SMAD1. Has an essential function in negative selection of thymocytes, perhaps by coupling NCK1 to activation of JNK1.

Isoform 4 can activate the JNK pathway. Involved in the regulation of actin cytoskeleton reorganization, cell-matrix adhesion, cell-cell adhesion and cell migration.

翻译修饰:

Autophosphorylated.

细胞定位:

Cytoplasm. Cell junction>Synapse>Postsynaptic density. Cell projection>Axon. Cell projection>Dendrite.

Golgi apparatus.

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionSubcellular location
组织特异性:

Expressed in the brain, isoform 2 is more abundant than isoform 1. Isoform 3 is ubiquitously expressed. Isoform 1 is most abundant in the skeletal muscle. Isoform 4 is ubiquitously expressed with relative high levels in brain, skeletal muscle, pancreas and testis.

亚基结构:

Interacts with TANC1 (By similarity). Interacts with RAP2A. Isoform 4 interacts with NCK1.

蛋白家族:

Belongs to the protein kinase superfamily. STE Ser/Thr protein kinase family. STE20 subfamily.

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