PDE4B/C/D Antibody - #AF9151

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产品: PDE4B/C/D 抗体
货号: AF9151
描述: Rabbit polyclonal antibody to PDE4B/C/D
应用: WB IHC IF/ICC
反应: Human, Mouse
分子量: 76kDa; 83kD,80kD,91kD(Calculated).
蛋白号: Q07343 | Q08493 | Q08499
RRID: AB_2843341

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产品描述

来源:
Rabbit
应用:
WB 1:1000, IHC 1:50-1:200, IF/ICC 1:100-1:500
*The optimal dilutions should be determined by the end user.
*Tips:

WB: 适用于变性蛋白样本的免疫印迹检测. IHC: 适用于组织样本的石蜡(IHC-p)或冰冻(IHC-f)切片样本的免疫组化/荧光检测. IF/ICC: 适用于细胞样本的荧光检测. ELISA(peptide): 适用于抗原肽的ELISA检测.

反应:
Human,Mouse
克隆:
Polyclonal
特异性:
PDE4B/C/D Antibody detects endogenous levels of total PDE4B/C/D.
RRID:
AB_2843341
引用格式: Affinity Biosciences Cat# AF9151, RRID:AB_2843341.
偶联:
Unconjugated.
纯化:
The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).
保存:
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
别名:

展开/折叠

5''-cyclic phosphodiesterase 4B; cAMP-specific 3''; cAMP-specific 3',5'-cyclic phosphodiesterase 4B; cAMP-specific phosphodiesterase-4 B isoform; DKFZp686F2182; DPDE4; dunce-like phosphodiesterase E4; MGC126529; OTTHUMP00000010652; OTTHUMP00000010653; OTTHUMP00000010654; OTTHUMP00000010656; OTTHUMP00000232365; PDE 4B; PDE IVB; PDE32; Pde4b; PDE4B_HUMAN; PDE4B5; PDEIVB; Phosphodiesterase 4B; Phosphodiesterase 4B cAMP specific; Phosphodiesterase 4B, cAMP specific (phosphodiesterase E4 dunce homolog, Drosophila); 5''-cyclic phosphodiesterase 4C; cAMP specific 3' 5' cyclic phosphodiesterase 4C; cAMP-specific 3''; DPDE 1; DPDE1; MGC126222; PDE 21; PDE 4C; PDE21; Pde4c; PDE4C_HUMAN; Phosphodiesterase 4C; Phosphodiesterase 4C cAMP specific; Phosphodiesterase 4C, cAMP specific (phosphodiesterase E1 dunce homolog, Drosophila); Phosphodiesterase E1 dunce homolog; Phosphodiesterase E1 dunce homolog Drosophila; Phosphodiesterase type 4C; cAMP specific 3',5' cyclic phosphodiesterase 4D; DKFZp686M11213; 5''-cyclic phosphodiesterase 4D; cAMP specific phosphodiesterase 4D; cAMP specific phosphodiesterase PDE4D6; cAMP-specific 3''; DPDE3; Dunce like phosphodiesterase E3; FLJ97311; HSPDE4D; PDE43; Pde4d; PDE4D_HUMAN; PDE4DN2; Phosphodiesterase 4D cAMP specific (dunce (Drosophila) homolog phosphodiesterase E3); Phosphodiesterase 4D cAMP specific (phosphodiesterase E3 dunce homolog Drosophila); Phosphodiesterase 4D cAMP specific; Phosphodiesterase 4D, cAMP specific (dunce); STRK1;

抗原和靶标

免疫原:
Uniprot:

Q07343(PDE4B_HUMAN) >>Visit HPA database.

Q08493(PDE4C_HUMAN) >>Visit HPA database.

Q08499(PDE4D_HUMAN) >>Visit HPA database.

基因/基因ID:
PDE4B(5142) | PDE4C(5143) | PDE4D(5144)
表达:
Q07343 PDE4B_HUMAN:

Expressed in brain, heart, lung and skeletal muscle.

Q08493 PDE4C_HUMAN:

Expressed in various tissues but not in cells of the immune system.

Q08499 PDE4D_HUMAN:

Expressed in colonic epithelial cells (at protein level). Widespread; most abundant in skeletal muscle. Isoform 6 is detected in brain. Isoform 8 is detected in brain, placenta, lung and kidney. Isoform 7 is detected in heart and skeletal muscle.

序列:
MKKSRSVMTVMADDNVKDYFECSLSKSYSSSSNTLGIDLWRGRRCCSGNLQLPPLSQRQSERARTPEGDGISRPTTLPLTTLPSIAITTVSQECFDVENGPSPGRSPLDPQASSSAGLVLHATFPGHSQRRESFLYRSDSDYDLSPKAMSRNSSLPSEQHGDDLIVTPFAQVLASLRSVRNNFTILTNLHGTSNKRSPAASQPPVSRVNPQEESYQKLAMETLEELDWCLDQLETIQTYRSVSEMASNKFKRMLNRELTHLSEMSRSGNQVSEYISNTFLDKQNDVEIPSPTQKDREKKKKQQLMTQISGVKKLMHSSSLNNTSISRFGVNTENEDHLAKELEDLNKWGLNIFNVAGYSHNRPLTCIMYAIFQERDLLKTFRISSDTFITYMMTLEDHYHSDVAYHNSLHAADVAQSTHVLLSTPALDAVFTDLEILAAIFAAAIHDVDHPGVSNQFLINTNSELALMYNDESVLENHHLAVGFKLLQEEHCDIFMNLTKKQRQTLRKMVIDMVLATDMSKHMSLLADLKTMVETKKVTSSGVLLLDNYTDRIQVLRNMVHCADLSNPTKSLELYRQWTDRIMEEFFQQGDKERERGMEISPMCDKHTASVEKSQVGFIDYIVHPLWETWADLVQPDAQDILDTLEDNRNWYQSMIPQSPSPPLDEQNRDCQGLMEKFQFELTLDEEDSEGPEKEGEGHSYFSSTKTLCVIDPENRDSLGETDIDIATEDKSPVDT

MENLGVGEGAEACSRLSRSRGRHSMTRAPKHLWRQPRRPIRIQQRFYSDPDKSAGCRERDLSPRPELRKSRLSWPVSSCRRFDLENGLSCGRRALDPQSSPGLGRIMQAPVPHSQRRESFLYRSDSDYELSPKAMSRNSSVASDLHGEDMIVTPFAQVLASLRTVRSNVAALARQQCLGAAKQGPVGNPSSSNQLPPAEDTGQKLALETLDELDWCLDQLETLQTRHSVGEMASNKFKRILNRELTHLSETSRSGNQVSEYISRTFLDQQTEVELPKVTAEEAPQPMSRISGLHGLCHSASLSSATVPRFGVQTDQEEQLAKELEDTNKWGLDVFKVAELSGNRPLTAIIFSIFQERDLLKTFQIPADTLATYLLMLEGHYHANVAYHNSLHAADVAQSTHVLLATPALEAVFTDLEILAALFASAIHDVDHPGVSNQFLINTNSELALMYNDASVLENHHLAVGFKLLQAENCDIFQNLSAKQRLSLRRMVIDMVLATDMSKHMNLLADLKTMVETKKVTSLGVLLLDNYSDRIQVLQNLVHCADLSNPTKPLPLYRQWTDRIMAEFFQQGDRERESGLDISPMCDKHTASVEKSQVGFIDYIAHPLWETWADLVHPDAQDLLDTLEDNREWYQSKIPRSPSDLTNPERDGPDRFQFELTLEEAEEEDEEEEEEGEETALAKEALELPDTELLSPEAGPDPGDLPLDNQRT

MEAEGSSAPARAGSGEGSDSAGGATLKAPKHLWRHEQHHQYPLRQPQFRLLHPHHHLPPPPPPSPQPQPQCPLQPPPPPPLPPPPPPPGAARGRYASSGATGRVRHRGYSDTERYLYCRAMDRTSYAVETGHRPGLKKSRMSWPSSFQGLRRFDVDNGTSAGRSPLDPMTSPGSGLILQANFVHSQRRESFLYRSDSDYDLSPKSMSRNSSIASDIHGDDLIVTPFAQVLASLRTVRNNFAALTNLQDRAPSKRSPMCNQPSINKATITEEAYQKLASETLEELDWCLDQLETLQTRHSVSEMASNKFKRMLNRELTHLSEMSRSGNQVSEFISNTFLDKQHEVEIPSPTQKEKEKKKRPMSQISGVKKLMHSSSLTNSSIPRFGVKTEQEDVLAKELEDVNKWGLHVFRIAELSGNRPLTVIMHTIFQERDLLKTFKIPVDTLITYLMTLEDHYHADVAYHNNIHAADVVQSTHVLLSTPALEAVFTDLEILAAIFASAIHDVDHPGVSNQFLINTNSELALMYNDSSVLENHHLAVGFKLLQEENCDIFQNLTKKQRQSLRKMVIDIVLATDMSKHMNLLADLKTMVETKKVTSSGVLLLDNYSDRIQVLQNMVHCADLSNPTKPLQLYRQWTDRIMEEFFRQGDRERERGMEISPMCDKHNASVEKSQVGFIDYIVHPLWETWADLVHPDAQDILDTLEDNREWYQSTIPQSPSPAPDDPEEGRQGQTEKFQFELTLEEDGESDTEKDSGSQVEEDTSCSDSKTLCTQDSESTEIPLDEQVEEEAVGEEEESQPEACVIDDRSPDT

翻译修饰 - Q07343/Q08493/Q08499 作为底物

Site PTM Type Enzyme
Y47 Phosphorylation
S48 Phosphorylation
S114 Phosphorylation
S119 Phosphorylation
S124 Phosphorylation
S126 Phosphorylation
S131 Phosphorylation
S136 Phosphorylation
T164 Phosphorylation
S234 Phosphorylation
K238 Methylation
S291 Phosphorylation
T306 Phosphorylation
T499 Phosphorylation
S502 Phosphorylation
S641 Phosphorylation P28482 (MAPK1)
T646 Phosphorylation
Site PTM Type Enzyme
S18 Phosphorylation
S20 Phosphorylation
T25 Phosphorylation
S142 Phosphorylation
S171 Phosphorylation
S174 Phosphorylation
S190 Phosphorylation
S195 Phosphorylation
S197 Phosphorylation
Y199 Phosphorylation
S202 Phosphorylation
S214 Phosphorylation
S252 Phosphorylation
S255 Phosphorylation
S262 Phosphorylation
S299 Phosphorylation
S301 Phosphorylation
K307 Ubiquitination
K309 Methylation
T317 Phosphorylation
S325 Phosphorylation
S330 Phosphorylation
S334 Phosphorylation
K340 Ubiquitination
S348 Phosphorylation
K358 Ubiquitination
S362 Phosphorylation
S365 Phosphorylation
S375 Phosphorylation
K387 Sumoylation
K387 Ubiquitination
K396 Ubiquitination
K556 Ubiquitination
S576 Phosphorylation
S596 Phosphorylation
S597 Phosphorylation
S606 Phosphorylation
S657 Phosphorylation
S715 Phosphorylation P27361 (MAPK3) , P28482 (MAPK1)
S746 Phosphorylation
S761 Phosphorylation
Site PTM Type Enzyme
S54 Phosphorylation
S133 Phosphorylation P17612 (PRKACA)
S138 Phosphorylation
S140 Phosphorylation
Y142 Phosphorylation
S145 Phosphorylation
S197 Phosphorylation
S201 Phosphorylation
S214 Phosphorylation
Y215 Phosphorylation
K249 Ubiquitination
K251 Methylation
T259 Phosphorylation
S290 Phosphorylation
S309 Phosphorylation
S319 Phosphorylation
S520 Phosphorylation
K521 Acetylation
K536 Acetylation
K537 Acetylation
Y549 Phosphorylation
S601 Phosphorylation
S659 Phosphorylation P28482 (MAPK1)

研究背景

功能:

Hydrolyzes the second messenger cAMP, which is a key regulator of many important physiological processes. May be involved in mediating central nervous system effects of therapeutic agents ranging from antidepressants to antiasthmatic and anti-inflammatory agents.

组织特异性:

Expressed in brain, heart, lung and skeletal muscle.

蛋白家族:

Belongs to the cyclic nucleotide phosphodiesterase family. PDE4 subfamily.

功能:

Hydrolyzes the second messenger cAMP, which is a key regulator of many important physiological processes.

细胞定位:

Cell projection>Cilium.

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionSubcellular location
组织特异性:

Expressed in various tissues but not in cells of the immune system.

亚基结构:

Part of a complex containing AKAP5, ADCY5, ADCY6 and PKD2.

蛋白家族:

Belongs to the cyclic nucleotide phosphodiesterase family. PDE4 subfamily.

功能:

Hydrolyzes the second messenger cAMP, which is a key regulator of many important physiological processes.

翻译修饰:

Long isoforms that share a conserved PKA phosphorylation site in the N-terminus are activated by PKA through phosphorylation (By similarity). Isoform 3 and isoform 7 are activated by phosphorylation (in vitro), but not isoform 6. Isoform N3 and isoform 12 are phosphorylated on Ser-49, Ser-51, Ser-55 and Ser-59.

Sumoylation of long isoforms by PIAS4 augments their activation by PKA phosphorylation and represses their inhibition by ERK phosphorylation.

细胞定位:

Apical cell membrane. Cytoplasm. Membrane. Cytoplasm>Cytoskeleton. Cytoplasm>Cytoskeleton>Microtubule organizing center>Centrosome.
Note: Found in the soluble fraction, associated with membranes, and associated with the cytoskeleton and the centrosome (By similarity). Colocalized with SHANK2 to the apical membrane of colonic crypt cells.

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionSubcellular location
组织特异性:

Expressed in colonic epithelial cells (at protein level). Widespread; most abundant in skeletal muscle. Isoform 6 is detected in brain. Isoform 8 is detected in brain, placenta, lung and kidney. Isoform 7 is detected in heart and skeletal muscle.

亚基结构:

Homodimer for the long isoforms. Isoforms with truncated N-termini are monomeric. Isoform 3 is part of a ternary complex containing PRKAR2A, PRKAR2B and AKAP9. Interacts with PDE4DIP. Identified in a complex composed of RYR1, PDE4D, PKA, FKBP1A and protein phosphatase 1 (PP1) (By similarity). Isoform 5, isoform N3 and isoform 12 bind RACK1 via their unique N-terminus. Binds ARRB2. Interacts (via N-terminal region) with SHANK2 (via proline-rich region); the interaction is increased in a PKA-dependent manner.

蛋白家族:

Belongs to the cyclic nucleotide phosphodiesterase family. PDE4 subfamily.

研究领域

· Environmental Information Processing > Signal transduction > cAMP signaling pathway.   (View pathway)

· Human Diseases > Substance dependence > Morphine addiction.

· Metabolism > Nucleotide metabolism > Purine metabolism.

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